In Situ Sampling Tool for Assessing Bioavailability and Toxicity of Sediments

用于评估沉积物生物利用度和毒性的原位采样工具

• 批准号: 8227888
• 负责人: Nancy D Denslow
• 金额: $ 28.69万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-20 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8227888
• 关键词: Address; Adsorption; Amendment; Analytical Chemistry; Arizona; Behavior; Bioavailable; Biological; Biological Assay; Biological Availability; Body Burden; Carbon; Chemical Structure; Chemicals; Computer-Aided Design; Consumption; DNA Microarray Chip; Data; Data Analyses; Detection; Devices; Dichlorodiphenyl Dichloroethylene; Dieldrin; Effectiveness; Environment; Environmental Pollutants; Equilibrium; Family; Farming environment; Filtration; Fishes; Florida; Funding; Gene Expression; Goals; Health; Home environment; Hour; Human; In Situ; Laboratories; Laboratory Study; Legal patent; Mass Spectrum Analysis; Measurement; Measures; Methods; Microarray Analysis; Minnows; Modeling; Monitor; Organism; Parents; Pathway interactions; Performance; Phase; Procedures; Process; Protocols documentation; Pump; Risk; Sampling; Series; Signal Recognition Particle; Site; Solid; Stainless Steel; Superfund; Surface; System; Techniques; Technology; Testing; Time; Toxic effect; Triclosan; Tubular formation; Universities; Water; Water Pollutants; Work; adverse outcome; aged; base; bioaccumulation; design; exposed human population; field study; fipronil; high throughput screening; mathematical model; novel; novel strategies; physical property; pollutant; programs; prototype; receptor; remediation; research study; response; simulation; superfund site; tandem mass spectrometry; theories; tool; triclocarban; uptake; water flow; water sampling

DESCRIPTION (provided by applicant): ABSTRACT This project introduces a novel strategy for bioavailability determination of sediment-borne contaminants featuring a wide range of chemical structures, properties and physical-chemical behaviors. The project addresses the pressing need of Superfund stakeholders to determine in a convenient and reliable fashion both human health risks from contaminated sediments and the effectiveness of implemented remediation strategies. The in situ sampling/bioavailability determination (IS2B) tool is a novel, patent-pending device enabling simultaneous determination of contaminant levels in bulk water and pore water at hitherto unattainably low method detection limits (MDLs). When deployed, one half of the tubular IS2B device is buried in sediment and the other is exposed to bulk water. Integrated multi-channel pumps simultaneously draw bulk water and sediment pore water into the active sampling device and push it at, respectively, high and low desirable flow rates through an array of filters and adsorption media. Water samples either can be stored in the device or expelled into the bulk water at will. Due to unlimited access to pore and bulk water during deployment, the IS2B tool provides ultra-low MDLs for a broad spectrum of contaminants, ranging from fully water-soluble to highly sorptive and hydrophobic. This functionality distinguishes the device from presently available passive sampling strategies. Know-how from three previous SRP projects is being leveraged to test the working hypothesis that the IS2B technology can serve to reliably (i) sample bulk and pore water pollutants for analysis in the sub-ng/L range, (ii) inform on the bioavailability and bioactivity of sediment contaminants, (iii) inform on human exposures and associated health risks from fish consumption, and (iv) track the progress of sediment remediation activities. Laboratory and field studies will be conducted with contaminated sediments from Lake Apopka, home to one of Florida's various Superfund sites. To illustrate the breadth of IS2B applicability, this project concentrates on two traditional and three emerging sediment contaminants (p,p'-DDE, dieldrin versus fipronil, triclosan, triclocarban). Because there may be discrepancies between the bioaccumulation predicted via modeling from sampler data and actual bioaccumulation, bioavailability and bioaccumulation of pollutants will be assessed in lab experiments with freshly spiked and long-term aged contaminated sediments via determination of body burdens in two test organisms: Lumbriculus variegatus and Pimephales promelas. Biological responses to contaminant exposure will also be measured in fish using DNA microarray analysis to evaluate effects that may not be predicted solely based on body burden of parent compounds. Mathematical relationships between pollutant concentrations in bulk water, pore water, worms and fish will be formulated from theory and lab data, and applied to IS2B-derived data to predict risk and track the effectiveness of two remediation approaches, granular activated carbon (GAC) amendment and deep tilling of contaminated sediment. PUBLIC HEALTH RELEVANCE: Project Narrative This research project addresses the pressing need for better monitoring of toxicant bioavailability in contaminated sediments by introducing the in situ sampling/bioavailability determination (IS2B) approach. The proposed device and mathematical model will inform risk assessment for environmental management for Superfund and other hazardous waste sites.

描述（由申请人提供）：摘要该项目引入了一种新的策略，用于测定沉积物污染物的生物有效性，该污染物具有广泛的化学结构、性质和物理化学行为。该项目满足了超级基金利益相关者的迫切需求，即以方便可靠的方式确定受污染沉积物造成的人类健康风险以及所实施的修复策略的有效性。原位采样/生物利用度测定（IS2B）工具是一种新颖的、正在申请专利的设备，能够以迄今为止无法达到的低方法检测限（MDL）同时测定散装水和孔隙水中的污染物水平。部署时，管状 IS2B 装置的一半埋在沉积物中，另一半暴露在大量水中。集成的多通道泵同时将大量水和沉积物孔隙水吸入主动采样装置，并分别以高和低的理想流速推动其通过一系列过滤器和吸附介质。水样可以储存在设备中，也可以随意排入大量水中。由于在部署过程中可以不受限制地接触孔隙和大量水，IS2B 工具可为多种污染物（从完全水溶性到高吸着性和疏水性）提供超低的 MDL。该功能使该器件有别于目前可用的被动采样策略。正在利用之前三个 SRP 项目的专业知识来测试工作假设，即 IS2B 技术可以可靠地 (i) 对大容量和孔隙水污染物进行采样，以在亚纳克/升范围内进行分析，(ii) 提供有关沉积物污染物的生物利用度和生物活性，(iii) 通报人类接触鱼类的情况和相关健康风险，以及 (iv) 跟踪沉积物修复活动的进展。实验室和现场研究将利用来自阿波普卡湖的受污染沉积物进行，阿波普卡湖是佛罗里达州多个超级基金地点之一的所在地。为了说明 IS2B 适用性的广度，该项目重点关注两种传统的和三种新兴的沉积物污染物（p,p'-DDE、狄氏剂与氟虫腈、三氯生、三氯卡班）。由于通过采样器数据建模预测的生物累积量与实际生物累积量之间可能存在差异，因此将在实验室实验中通过确定两种测试生物体的身体负荷，使用新鲜加标和长期老化的污染沉积物来评估污染物的生物利用度和生物累积性： variegatus 和 Pimephales promelas。还将使用 DNA 微阵列分析来测量鱼类对污染物暴露的生物反应，以评估仅根据母体化合物的身体负荷无法预测的影响。散装水、孔隙水、蠕虫和鱼类中污染物浓度之间的数学关系将根据理论和实验室数据制定，并应用于 IS2B 派生数据，以预测风险并跟踪两种修复方法（颗粒活性炭 (GAC) 修正）的有效性以及受污染沉积物的深耕。 公共卫生相关性：项目叙述该研究项目通过引入原位采样/生物利用度测定（IS2B）方法，解决了更好地监测受污染沉积物中有毒物质生物利用度的迫切需要。拟议的设备和数学模型将为超级基金和其他危险废物场的环境管理风险评估提供信息。