Core C: Molecular Targets

核心 C：分子靶点

基本信息

批准号：
10686380
负责人：
H-H. Sherry CHOW
金额：
$ 30.35万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-10 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10686380
关键词：
Actinic keratosis Address Apoptotic Architecture Biological Biological Assay Biological Markers Biological Specimen Banks Biopsy Biostatistics Core Cells Clinical Trials Collaborations Collection Exposure to Formalin Freezing Hematoxylin and Eosin Staining Method Histopathology Human Institution Laboratories Light Maps Measurement Molecular Target Mus Paraffin Embedding Pathway interactions Phase Phosphoproteins Prevention Procedures Protein Microarray Assay Protein Microchips Proteins Protocols documentation Reproducibility Resource Development Resources Sampling Services Shipping Signal Transduction Signaling Protein Skin Skin Carcinoma Slide Specimen Stains Standardization Sunburn TLR4 gene Thick Tissue Microarray clinical development cost cost effective laser capture microdissection method development molecular marker mouse model novel programs sample collection skin damage skin squamous cell carcinoma sun damage sun protection tissue preparation tissue processing

项目摘要

ABSTRACT Core C (Chow, Petricoin) Molecular Targets The Molecular Targets Core is a multi-functional core. The Core provides a collection, tracking, storage, and distribution point for biological samples and is a resource and service for carrying out the necessary laboratory analyses proposed in this application. Furthermore, the Molecular Targets Core is an essential component of the Program Project in that it allows the Projects to address Specific Aims in a consistent, reproducible, and cost- effective manner with regards to the identification of molecular markers. The Core will interact in a significant manner with all Projects and the other cores. Additionally, the Core has put into place detailed forms and SOPs, and is an essential resource for development of clinical and murine protocols as well as sample management for a continually growing biospecimen repository. In collaboration with the Biostatistics Core (Core B), Molecular Targets Core will carry-out sample analyses and interact with each Project on the transfer and/or analysis of samples, as well as on the management and interpretation of the results. The Core provides immunohistochemical method development and analysis. The Core also provides Reverse Phase Protein Microarray (RPPA), a multiplexed quantitative measurement of multiple signaling proteins, many of which are phosphoproteins, from biological specimens. RPPA represents a novel means of quantitative measurement of hundreds of proteins from a single specimen.

抽象的核心 C（Chow、Petricoin）分子靶标分子靶标核心是一个多功能核心。核心提供收集、跟踪、存储和生物样本的分发点，是开展必要实验室的资源和服务本申请中提出的分析。此外，分子靶标核心是计划项目，因为它允许项目以一致的、可重复的和成本的方式解决特定目标分子标记鉴定的有效方法。核心将在重要的方面进行互动与所有项目和其他核心的方式。此外，核心还制定了详细的表格和标准操作程序，是开发临床和小鼠方案以及样本管理的重要资源不断增长的生物样本库。与生物统计学核心（核心 B）合作，分子 Targets Core 将进行样本分析，并与每个项目就传输和/或分析进行互动样本，以及结果的管理和解释。核心提供免疫组织化学方法开发和分析。核心还提供反相蛋白微阵列（RPPA），多种信号蛋白的多重定量测量，其中许多是磷蛋白，来自生物样本。 RPPA 代表了一种定量测量的新方法来自单个样本的数百种蛋白质。