Molecular Detection and Diagnostics Core

分子检测和诊断核心

• 批准号: 10664051
• 负责人: Tony Y. Hu
• 金额: $ 17.38万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-10 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10664051
• 关键词: APOL1 gene; Address; Analytical Chemistry; Basic Science; Biochemistry; Biology; Biomedical Engineering; Biosensor; Biotechnology; Blood Pressure; Cardiometabolic Disease; Cells; Centers of Research Excellence; Chronic; Chronic Disease; Chronic Kidney Failure; Clinical Research; Clinical Sciences; Collaborations; Communicable Diseases; Complex; Consultations; Core Facility; Detection; Development; Diagnosis; Diagnostic; Disease; Disease Progression; Doctor of Philosophy; Early Diagnosis; Etiology; Evaluation; Experimental Designs; Future; Genetic Markers; Goals; Human Resources; Image; Immune; Individual; Infrastructure; Institution; Intake; Interdisciplinary Study; Knowledge; Louisiana; Mass Spectrum Analysis; Methodology; Methods; Molecular; Molecular Analysis; Molecular Biology; Molecular Diagnosis; Monitor; Nanotechnology; Overweight; Pathologic; Phase; Pilot Projects; Population Sciences; Potassium; Prevention; Process; Proteome; Proteomics; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Sampling; Services; Technology; Tissues; Training; Translational Research; Universities; Whole Organism; X Inactivation; analytical method; biomarker discovery; biophysical techniques; blood pressure reduction; career development; early detection biomarkers; empowerment; experience; innovation; innovative technologies; instrument; instrumentation; molecular diagnostics; molecular marker; novel; novel marker; organ on a chip; personalized diagnostics; personalized medicine; point of care testing; professor; prognostic; programs; real time monitoring; research and development; screening; single cell analysis; skills; specific biomarkers; sustainable resource; synergism; treatment response

PROJECT SUMMARY/ABSTRACT (Molecular Detection and Diagnostics Core) The Tulane COBRE for Clinical and Translational Research in Cardiometabolic Diseases Phase 2 application aims to further develop and strengthen the clinical and translational research infrastructure at Tulane University and to continuously expand and support a critical mass of investigators with expertise in clinical and translational research in cardiometabolic diseases. The newly proposed Molecular Detection and Diagnostics Core (MDDC) will support the Research Project Leaders (RPLs), Pilot Project Leaders (PPLs), other COBRE investigators and collaborators at Tulane University by providing cutting-edge instruments, innovative technologies and methodologies, and expertise in multidisciplinary research including biochemistry, biomedical engineering, analytical chemistry, proteomics, nanotechnology, and mass spectrometry (MS) to advance their clinical and translational research in the etiology, prevention, diagnosis, and treatment of cardiometabolic disease. The MDDC will develop and adapt integrated nanotechnology-based strategies and other innovative technologies to identify novel biomarkers for early disease detection, prognostic evaluation, and the real-time monitoring of treatment responses as well as to understand the molecular mechanisms of cardiometabolic disease. The MDDC seeks to catalyze research that spans the analytical chemistry-biology interface, empowering investigators to identify and solve important interdisciplinary research questions in the clinical and translational sciences of cardiometabolic disease. Based on our team's expertise, the MDDC will provide COBRE investigators with training and services in state-of-the-art analytical technologies such as MS and microarray-based proteomics and organ-on-a-chip, among others, for the analysis of complex proteomes of tissue and biofluid samples. The MDDC will also identify appropriate existing analytical methods as well as adapt newly developed methods, often not available in the form of mature technologies. The MDDC will be housed within the Tulane University Center for Cellular and Molecular Diagnostics, which has been well established for infectious and chronic disease research. During the COBRE Phase 2, the MDDC will provide cutting-edge instruments and methodological support for RPLs of Projects 2 and 3 to conduct their individual research projects. In addition, the MDDC will provide expert consultation and state-of-the-art technologies for RPLs of Projects 1 and 4 to develop R01 applications to further investigate molecular mechanism of APOL1 on chronic kidney disease progression and potassium intake on blood pressure reduction. The proposed MDDC will enhance the interactions and collaborations among COBRE, non-COBRE and external investigators involved in molecular studies of cardiometabolic disease. Since the MDDC is a critical component of the COBRE program, our long-term goal is to grow MDDC into a sustainable resource that serves all phases of translational research, from basic science to clinical science to population science at Tulane University by providing a broad range of state-of-the-art cellular and molecular analytical services.

项目摘要/摘要（分子检测和诊断核心） 杜兰大学 COBRE 心脏代谢疾病临床和转化研究 2 期应用 旨在进一步发展和加强杜兰大学的临床和转化研究基础设施 并不断扩大和支持大量具有临床和转化专业知识的研究人员 心脏代谢疾病研究。新提出的分子检测和诊断核心（MDDC） 将支持研究项目负责人 (RPL)、试点项目负责人 (PPL)、其他 COBRE 研究人员和 杜兰大学的合作者提供尖端仪器、创新技术和 多学科研究的方法论和专业知识，包括生物化学、生物医学工程、 分析化学、蛋白质组学、纳米技术和质谱 (MS) 来推进其临床和 心脏代谢疾病的病因学、预防、诊断和治疗的转化研究。这 MDDC 将开发和调整基于纳米技术的综合战略和其他创新技术，以 识别用于早期疾病检测、预后评估和实时监测的新型生物标志物 治疗反应以及了解心脏代谢疾病的分子机制。 MDDC 寻求促进跨越分析化学-生物学界面的研究，使研究人员能够 识别并解决临床和转化科学中重要的跨学科研究问题 心脏代谢疾病。根据我们团队的专业知识，MDDC 将为 COBRE 调查人员提供 最先进的分析技术（例如 MS 和基于微阵列的蛋白质组学）的培训和服务 和器官芯片等，用于分析组织和生物流体样品的复杂蛋白质组。这 MDDC 还将确定适当的现有分析方法并采用新开发的方法，通常 无法以成熟技术的形式提供。 MDDC 将设在杜兰大学中心内 用于细胞和分子诊断，已在传染病和慢性疾病方面得到了很好的应用 研究。在 COBRE 第 2 阶段期间，MDDC 将提供尖端工具和方法论 支持项目 2 和 3 的 RPL 开展各自的研究项目。此外，MDDC 将 为项目1和4的RPL提供专家咨询和最先进的技术来开发R01 应用进一步研究APOL1对慢性肾脏病进展的分子机制和 钾摄入量对血压的降低。拟议的 MDDC 将加强互动和 COBRE、非 COBRE 和参与分子研究的外部研究人员之间的合作 心脏代谢疾病。由于 MDDC 是 COBRE 计划的重要组成部分，因此我们的长期目标是 将 MDDC 发展成为一个可持续的资源，服务于从基础科学到转化研究的各个阶段 杜兰大学通过提供广泛的最先进的细胞研究，从临床科学到人口科学 和分子分析服务。