Mount Sinai HHEAR Network Targeted Lab Hub

西奈山 HHEAR 网络目标实验室中心

• 批准号: 10875017
• 负责人: Robert O Wright
• 金额: $ 12.96万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10875017
• 关键词: Address; Administrative Supplement; Age; Analytical Chemistry; Automatic Data Processing; Biological; Biological Assay; Biological Markers; Blinded; Chemical Exposure; Chemicals; Chemistry; Child; Chromatography; Client; Clinical Medicine; Clinical Trials; Collaborations; Complex; Counseling; Data; Data Analyses; Data Reporting; Database Management Systems; Disease; Dose; Elderly; Elements; Enrollment; Ensure; Environment; Etiology; Europe; Exposure to; Food; Future; Genes; Genomics; Geographic Locations; Goals; Grant; Hair; Health; Human; Human Development; Human Genome; Informatics; Institution; International; Label; Laboratories; Life Cycle Stages; Liquid substance; Management Information Systems; Mass Spectrum Analysis; Measures; Medicine; Metadata; Metals; Methods; Mexico; Monitor; Occupations; Organic Chemicals; Outcome; Parents; Pattern; Pesticides; Phenotype; Population; Predisposition; Pregnancy; Pregnant Women; Preparation; Procedures; Protocols documentation; Quality Control; Randomized; Reporting; Research; Research Design; Research Personnel; Resources; Risk; Robotics; Sampling; Science; Services; Shapes; Software Design; Specimen; Structure; System; Techniques; Technology; Testing; Time; Tooth structure; Toxicology; U-Series Cooperative Agreements; United States; United States National Institutes of Health; Urine; Woman; Work; autism spectrum disorder; computerized data processing; data centers; data integrity; design; detection limit; early childhood; experience; human error; human population study; innovation; instrument; meetings; method development; multidisciplinary; outreach; pesticide exposure; prenatal; prevent; programs; quality assurance; response; software systems; symposium

Project Summary The Targeted Analysis Resource is a multi-disciplinary, full service exposure biomarker program designed to integrate inorganic and organic environmental measures into existing NIH studies. In this supplement we now extend services to include exposure to pesticides that pose health risk to women and children during critical windows such as pregnancy and early childhood. These pesticides are not readily measured in urine due to short half-lives but we have developed methods to measure them in hair. We hold weekly Executive Steering Committee meetings of the full Lab Hub team to discuss jobs, troubleshoot workflows, and prioritize resources and activities in order to provide state of the art service to HHEAR clients. Our team has considerable expertise in matching the underlying toxicology of targeted exposure biomarkers to the study design, to the biological specimen types collected, and to the health phenotypes measured, in this case hair pesticides. Our lab feasibility assessments help shape the science of a client’s submission towards higher impact studies. We also counsel clients on preventing the use of precious samples on assays that are deemed likely to be below detection limits based on our many years of research experience and understanding of exposure scenarios. We work with the Administrative Core to coordinate services, including sample management, receiving, labeling and tracking of the hair samples. We serve as expert consultants with the Coordinating Center, PI/Clients and other HHEAR Resources. We collaborate with the Data Center in the management of results reporting, ensuring that data are properly formatted and have sufficient metadata, as well as participate in the analysis of exposure/phenotype relationships. We use automated robotic sample handling and preparation methods, high throughput analytical procedures and state of the art inter-laboratory QA/QC procedures which include real time chromatography monitoring and database management through state-of-the-art laboratory information management system (LIMS) software. We also automated data processing to reduce human error. Our methods ensure proper sample tracking and data reporting and avoid mismatches of samples to results. With substantial institutional resources at its disposal, the Targeted Resource has more than doubled the number of mass spectrometry instruments to 16 since our inception, ensuring our capacity to serve the needs of the HHEAR Network. Our team also conducts extensive outreach and is the only HHEAR lab hub to organize an international Exposome Symposia in the US, Europe and Mexico, in collaboration with the Mount Sinai Untargeted Lab Hub. QA/QC protocols and standard operating procedures for all target compounds, including the pesticides that are the focus of this application, have been established to maintain the rigor of our chemical analyses. We developed panels for elements and organic chemicals that in sum assess more than 200 chemicals. We participate in all HHEAR mandated QA/QC and PT programs for metals and organic chemicals to verify our precision and accuracy.

项目概要 靶向分析资源是一个多学科、全方位服务的暴露生物标志物计划，旨在 我们现在将无机和有机环境措施纳入现有的 NIH 研究中。 扩大服务范围，包括在关键时期接触对妇女和儿童构成健康风险的农药 由于以下原因，这些农药不易在尿液中检测到。 半衰期很短，但我们已经开发出在头发中测量半衰期的方法，我们每周举行一次行政指导。 整个实验室中心团队召开委员会会议，讨论工作、解决工作流程问题并确定资源优先级 和活动，以便为 HHEAR 客户提供最先进的服务。 将目标暴露生物标志物的基本毒理学与研究设计相匹配的专业知识 收集生物样本类型，并测量健康表型，在本例中为我们的毛发杀虫剂。 实验室评估可行性有助于塑造客户提交的更具影响力的研究的科学性。 还建议客户避免在被认为可能低于以下标准的检测中使用珍贵样品 检测限值基于我们多年的研究经验和对暴露场景的理解。 我们与行政核心合作协调服务，包括样品管理、接收、 我们作为协调中心的专家顾问，对头发样本进行标记和跟踪。 PI/客户和其他 HHEAR 资源我们与数据中心合作管理结果。 报告，确保数据格式正确并具有足够的元数据，并参与 我们使用自动化机器人样品处理和制备来分析暴露/表型关系。 方法、高通量分析程序和最先进的实验室间 QA/QC 程序， 包括通过最先进的实验室进行实时色谱监测和数据库管理 我们还自动化数据处理以减少人为错误。 我们的方法可确保正确的样本跟踪和数据报告，并避免样本与结果不匹配。 凭借大量可供支配的机构资源，目标资源已增加了一倍多 自成立以来，质谱仪器数量已增至 16 台，确保我们有能力满足需求 我们的团队还开展广泛的外展活动，并且是唯一开展此类活动的 HHEAR 实验室中心。 与 Mount 合作，在美国、欧洲和墨西哥举办国际暴露组研讨会 西奈非目标实验室中心。所有目标化合物的 QA/QC 协议和标准操作程序， 包括本申请重点关注的农药，其建立是为了保持我们的严格性 我们开发了元素和有机化学品面板，总体评估超过 我们参与了 200 种化学品的所有 HHEAR 强制 QA/QC 和 PT 计划。 化学物质来验证我们的精度和准确性。

项目成果

Hormones and women's respiratory health across the lifespan: Windows of opportunity for advancing research.

激素和女性整个生命周期的呼吸健康：推进研究的机会之窗。

• 发表时间: 2021
• 作者: Wright; Rosalind J
• 通讯作者: Rosalind J

Racial/ethnic and neighborhood disparities in metals exposure during pregnancy in the Northeastern United States.

美国东北部怀孕期间金属暴露的种族/民族和社区差异。

• 发表时间: 2022-05-10
• 作者: Geron, Mariel;Cowell, Whitney;Amarasiriwardena, Chitra;Andra, Syam S;Carroll, Kecia;Kloog, Itai;Wright, Robert O;Wright, Rosalind J
• 通讯作者: Wright, Rosalind J

Per- and polyfluoroalkyl substances (PFAS) exposure and thyroid cancer risk.

全氟烷基物质和多氟烷基物质 (PFAS) 暴露与甲状腺癌风险。

• 发表时间: 2023-11
• 影响因子: 11.1
• 作者: van Gerwen, Maaike;Colicino, Elena;Guan, Haibin;Dolios, Georgia;Nadkarni, Girish N;Vermeulen, Roel C H;Wolff, Mary S;Arora, Manish;Genden, Eric M;Petrick, Lauren M
• 通讯作者: Petrick, Lauren M

Studying the Exposome to Understand the Environmental Determinants of Complex Liver Diseases.

研究暴露组以了解复杂肝病的环境决定因素。

• 发表时间: 2020
• 作者: Cheung, Angela C;Walker, Douglas I;Juran, Brian D;Miller, Gary W;Lazaridis, Konstantinos N
• 通讯作者: Lazaridis, Konstantinos N

Minimally Invasive Biospecimen Collection for Exposome Research in Children's Health.

用于儿童健康暴露组研究的微创生物样本采集。

• 发表时间: 2020
• 影响因子: 7.9
• 作者: Petrick, Lauren M;Arora, Manish;Niedzwiecki, Megan M
• 通讯作者: Niedzwiecki, Megan M