Biomarker and Analytical Chemistry Core

生物标志物和分析化学核心

• 批准号: 10665901
• 负责人: JOHN P RICHIE
• 金额: $ 113.71万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-30 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10665901
• 关键词: 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol; 8-hydroxy-2' -deoxyguanosine; Acetylcysteine; Aerosols; Analytical Chemistry; Animals; Archives; Benzo(a)pyrene; Biological; Biological Markers; Blood; Cells; Characteristics; Chemicals; Clinical; Clinical Chemistry; Clinical Research; Collaborations; Collection; Communities; DNA Adduction; DNA Adducts; Data Analyses; Development; Disease; Educational Activities; Educational process of instructing; Electronic cigarette; Emerging Tobacco Products; Exhalation; Glutathione; High Pressure Liquid Chromatography; Human; Human Resources; IL6 gene; Inflammation; Isoprostanes; Laboratories; Laboratory Animal Models; Length; Maintenance; Mass Fragmentography; Measurement; Measures; Mentorship; Methodology; Methods; Mission; Modeling; Nicotine; Oral mucous membrane structure; Organic Chemistry; Oxidation-Reduction; Pattern; Phospholipids; Physical assessment; Physical condensation; Pilot Projects; Play; Postdoctoral Fellow; Preparation; Protocols documentation; Research; Research Design; Research Personnel; Research Support; Resources; Role; Saliva; Sampling; Science; Smoke; Smoking; Stable Isotope Labeling; Standardization; TNF gene; Techniques; Time; Tobacco; Tobacco smoke; Tobacco use; Toxic effect; Training; Urine; Weight; Work; addiction; analytical method; biological specimen archives; carcinogenicity; career; chemical property; clinical investigation; design; electronic liquid; instrumentation; investigator training; method development; novel; physical property; tobacco products; tobacco regulatory science; tobacco user; vaping; ventilation

Project Summery / Abstract: Biomarker and Analytical Chemistry Core The PSU TCORS includes the three major interdisciplinary projects, pilot projects and educational activities that will rely heavily on the use of analytical laboratory measurements for the assessment of tobacco products and biomarkers analyses for the assessment of the effects of tobacco-related exposures on tobacco product users and laboratory animal models of tobacco related harm. To support these activities, the Biomarkers and Analytical Chemistry Core (BAC) will play a pivotal role. The BAC was established in 2013 as part of our original TCORS and, since that time, has continued to support the Center activities by providing expertise for study design, sample processing and analyses of tobacco products and related biomarkers, methods development and data interpretation. We plan to continue with these activities to meet the needs of the research to be conducted in the main and pilot project of the current proposal. As such, the BAC focuses on the use of analytical chemistry and clinical chemistry methodologies supported by a strong expertise in synthetic organic chemistry. The BAC activities will include the use of standardized analytical techniques and instrumentation as well as the development of new analytical methods and design of novel instrumentation for use with new and emerging tobacco products. In addition to supporting the research mission, the BAC will also play a key role in the educational activities of the Center in collaboration with the Career Enhancement Core by providing mentorship and training for TCORS postdoctoral scholars and teaching a 3-credit course on the use of tobacco biomarkers (PHS 684 Biomarkers in Tobacco Research). The BAC will support the three major projects, pilot projects, and educational activities by focusing on 8 specific aims. These include (1) measurement of toxic and carcinogenic agents in tobacco products and their resulting smoke/aerosols; (2) maintenance of experimental smoking laboratories with the capability of machine smoking/vaping a wide variety of tobacco products for chemical analysis or animal exposure studies; (3) quantification of biomarkers in biological samples from animal or clinical studies; (4) assessment of physical and chemical characteristics of tobacco products; (5) preparation of synthetic standards required for chemical analyses; (6) processing and archival of biological samples; (7) development of new methods and instrumentation for assessing tobacco- derived agents in new and emerging products; and (8) training of TCORS investigators on the measurement and use of biomarkers and their interpretation from both a research and regulatory perspective.

项目总结/摘要：生物标志物和分析化学核心 PSU TCORS 包括三大跨学科项目、试点项目和教育活动 这将在很大程度上依赖于使用分析实验室测量来评估烟草产品 和生物标志物分析，用于评估烟草相关暴露对烟草产品的影响 烟草相关危害的使用者和实验动物模型。为了支持这些活动，生物标志物和 分析化学核心（BAC）将发挥关键作用。 BAC 成立于 2013 年，是我们的一部分 最初的 TCORS，从那时起，一直通过提供专业知识来继续支持中心的活动 烟草制品和相关生物标志物的研究设计、样品处理和分析、方法 开发和数据解释。我们计划继续开展这些活动，以满足人们的需求 将在当前提案的主要项目和试点项目中进行研究。因此，北仲的重点是 在强大的专业知识的支持下使用分析化学和临床化学方法 有机合成化学。 BAC 活动将包括使用标准化分析技术和 仪器仪表以及新分析方法的开发和新型仪器的设计 与新兴烟草产品一起使用。除了支持研究任务外，北仲还将 与职业发展核心合作，在中心的教育活动中发挥关键作用 为 TCORS 博士后学者提供指导和培训，并教授 3 学分的使用课程 烟草生物标志物（PHS 684 烟草研究中的生物标志物）。北仲将支持三大 项目、试点项目和教育活动，重点关注 8 个具体目标。其中包括 (1) 测量烟草制品中的有毒和致癌物质及其产生的烟雾/气溶胶； (2) 维护具有机器吸烟/电子烟能力的实验吸烟实验室 用于化学分析或动物暴露研究的各种烟草产品； (3) 生物标志物的定量 来自动物或临床研究的生物样本； (4)理化特性评估 烟草制品； (5) 化学分析所需的合成标准品的制备； (6)加工及 生物样本的归档； (7) 开发评估烟草的新方法和仪器 新兴产品的派生代理； (8) 对 TCORS 研究人员进行测量培训 生物标志物的使用及其从研究和监管角度的解释。