VIRUS CHALLENGE STOCK PRODUCTION AND STORAGE

病毒挑战库存的生产和储存

• 批准号: 7716235
• 负责人: Preston A Marx
• 金额: $ 6.46万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-21 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7716235
• 关键词: Aerosols; Animals; CV-1; Cell Line; Cells; Computer Retrieval of Information on Scientific Projects Database; Cryopreservation; Databases; Exhalation; Freezing; Funding; Grant; Institution; Liquid substance; Location; Nitrogen; Numbers; Oryctolagus cuniculus; Plaque Assay; Preparation; Production; Rabbit Pox Virus; Records; Research; Research Personnel; Resources; SIV; Serum; Source; Tissues; Titrations; United States National Institutes of Health; Vaccines; Vial device; Viral; Virus; cold temperature; cost; design; genetic pedigree; prevent; simian human immunodeficiency virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The core is for the production and cryopreservation of virus stocks to be used by NIH funded investigators. The core is designed to avoid duplication of effort and cost in the preparation of virus stocks, particularly SIV and SHIV. The viral stocks were all quantified by TCID50 in the appropriate cell line. Thus, the core prevents the unnecessary duplication of effort and expense among investigators at the TNPRC. The core also maintains records on the pedigree of all virus stocks. In 2007, 29 virus stocks were stored in Liquid Nitrogen and 51 in ultra low temperature freezers at the TNPRC. An Excel database with details on the frozen stocks was maintained and includes: date frozen, titration date, TCID50, the number of vials of each virus and the exact storage location. In this past year the core has supplied 100 vials of different viral stocks. Of those stocks 45 were prepared in 2007 as a result of specific investigator requests. Production and characterization of stocks of Rabbitpox were done as part of a vaccine study. Two Rabbitpox virus challenge stocks were prepared in CV-1 cells. All of the first stock was used for aerosol inoculations of 4 rabbits in Group 1. The second stock was and used for aerosol inoculation of Group 2, 3, & 4, each of which consisted of 12 animals. Viral isolation and quatiffacation for Rabbitpox was performed on the tissues, exhaled breath, and serum collected from the animals. Quantification was done by plaque assay.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 核心是为了生产和冷冻保存病毒库存，由NIH资助的研究人员使用。 该核心旨在避免在准备病毒库存（特别是SIV和SHIV）制备方面的努力和成本。 病毒量均由适当的细胞系中的TCID50定量。 因此，核心阻止了TNPRC调查人员之间不必要的努力和费用的重复。该核心还保留了所有病毒库存的血统的记录。 2007年，在TNPRC的超低温度冷冻机中存储了29个病毒量，51个病毒储存。 Excel数据库，其中包含有关冷冻库存的详细信息，其中包括：日期冷冻，滴定日期，TCID50，每种病毒的小瓶数量和确切的存储位置。 在过去的一年中，核心提供了100瓶不同的病毒库存。 在2007年，由于特定的研究者要求，在这些股票中有45股。 作为疫苗研究的一部分，进行了兔子股的生产和表征。 在CV-1细胞中制备了两个兔病毒挑战量。 所有第一个股票用于第1组4只兔子的气溶胶接种。第二股库存是对第2、3和4组的气溶胶接种，每种股票由12只动物组成。 在组织上进行了病毒分离和对兔子的quatiffacation，呼出的呼吸和从动物中收集的血清。 通过斑块测定进行定量。