DNA VACCINE FOR INDUCTION OF MUCOSAL IMMUNITY

用于诱导粘膜免疫的 DNA 疫苗

• 批准号: 8172993
• 负责人: Preston A Marx
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172993
• 关键词: Animals; Antigens; Aspirate substance; Biopsy; Blood; Computer Retrieval of Information on Scientific Projects Database; DNA; Data; Electroporation; Female; Funding; Grant; Immunization; Influenza A Virus, H1N1 Subtype; Inguinal lymph node group; Institution; Intramuscular Injections; Irrigation; Macaca mulatta; Mucosal Immunity; Research; Research Personnel; Resource Allocation; Resources; SIV; Sampling; Shipping; Ships; Source; Time; Tissue Sample; United States National Institutes of Health; Vaccines; Vagina; Work; base; chemokine; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Experiment 1: Twenty female Indian origin Rhesus macaques were assigned in 2008 to one of four experimental groups. All animals were previously immunized four times with DNA and various chemokines. Based on immunological data obtained from samples collected during the first four immunizations, it was decided to administer a fifth immunization. This immunization would be modified to include SIV nef/rev and H1N1 HA antigens. Three weeks prior to the fifth immunization (October 26-30, 2009), blood, fecal and BAL samples were collected from all 20 animals and shipped to UPenn. Tissue samples (to include endoscopic pinch biopsies, duodenal aspirates, bronchoaveolar lavages, inguinal lymph node biopsies, vaginal biopsies and vaginal washes) were also collected at this time. The fifth immunization was administered on November 16, 2009 via intramuscular injection followed immediately by electroporation as before. Blood and fecal samples were collected immediately prior to, one week post- and 3 weeks post-immunization. Tissue samples were also collected 3 weeks post-immunization. Experiment 2: A request to the Tulane Resource Allocation Committee was submitted in September 2009 for the acquisition of 24 female Indian origin Rhesus macaques for the next experiment in this project. Six animals were assigned in October and five animals were assigned in December. We are currently waiting for the remaining 13 animals to begin experimental work.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 实验1： 2008年将二十个印度恒河猕猴分配给了四个实验组之一。 先前用DNA和各种趋化因子对所有动物进行了四次免疫。基于从前四个免疫中收集的样品获得的免疫数据，决定进行第五次免疫。 这种免疫将被修改为包括SIV NEF/REV和H1N1 HA抗原。 在第五次免疫之前三周（2009年10月26日至30日），从所有20只动物中收集了血液，粪便和BAL样品，并运送到Upenn。 还收集了组织样品（包括内窥镜捏活活检，十二指肠抽吸物，支气管腔厕所，腹股沟淋巴结活检，阴道活检和阴道洗涤物）。 2009年11月16日通过肌肉注射进行了第五次免疫，然后立即进行电穿孔。 免疫后一周和3周之前，立即收集血液和粪便样品。 免疫后3周还收集组织样品。 实验2： 2009年9月，提交了向图兰资源分配委员会的请求，要求收购24位女性印度人恒河猕猴作为该项目的下一个实验。 十月分配了六只动物，并于12月分配了五只动物。 我们目前正在等待其余13只动物开始实验性工作。