VIRUS CHALLENGE STOCK PRODUCTION AND STORAGE

病毒挑战库存的生产和储存

• 批准号: 8358057
• 负责人: Preston A Marx
• 金额: $ 5.78万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358057
• 关键词: Cell Line; Chad; Cryopreservation; Databases; Freezing; Funding; Grant; HIV-2; Head; Liquid substance; Location; Microbiology; National Center for Research Resources; Nitrogen; Preparation; Primates; Principal Investigator; Production; Rabbit Pox Virus; Records; Research; Research Infrastructure; Research Personnel; Resources; SIV; Source; Titrations; United States National Institutes of Health; Vial device; Viral; Virus; biodefense; cold temperature; cost; design; genetic pedigree; prevent; programs; simian human immunodeficiency virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The core is for the production and cryopreservation of virus stocks to be used by NIH funded investigators. The core is designed to avoid duplication of effort and cost in the preparation of virus stocks, particularly SIV and SHIV. The viral stocks were all quantified by TCID50 in the appropriate cell line. Thus, the core prevents the unnecessary duplication of effort and expense among investigators at the TNPRC. The core maintains records on the pedigree of all virus stocks. In 2010, 61 virus stocks were stored in Liquid Nitrogen and 44 in ultra low temperature freezers at the TNPRC. An Excel database with details on the frozen stocks was maintained and includes: date frozen, titration date, TCID50, the number of vials of each virus and the exact storage location. In the past year, the core has supplied 46 vials of different viral stocks. Of those stocks, 31 vials were prepared in 2010 as a result of specific investigator requests. Several stocks of three different viruses were also prepared and/or added to the core in this year. They include RT-SHIV, SIV.SIVhybrid and HIV-2. Over the past year, 1 Rabbitpox virus challenge stock was stored in Liquid Nitrogen at the TNPRC. This program represents expansion of the core to include other viruses at the TNPRC. Rabbitpox is part of a biodefense research program headed by Dr. Chad Roy also in the Division of Microbiology at the TNPRC.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 核心是为了生产和冷冻保存病毒库存，由NIH资助的研究人员使用。 该核心旨在避免在准备病毒库存（特别是SIV和SHIV）制备方面的努力和成本。 病毒量均由适当的细胞系中的TCID50定量。 因此，核心阻止了TNPRC调查人员之间不必要的努力和费用的重复。 核心保留了所有病毒库存的血统的记录。 2010年，TNPRC的超低温度冷冻机中存储了61个病毒量，44个病毒储存。 Excel数据库，其中包含有关冷冻库存的详细信息，其中包括：日期冷冻，滴定日期，TCID50，每种病毒的小瓶数量和确切的存储位置。 在过去的一年中，核心提供了46瓶不同的病毒库存。 在这些股票中，由于特定的研究者要求，2010年准备了31个小瓶。 今年还准备了几种三种不同病毒的库存和/或添加到核心中。 它们包括RT-SHIV，SIV.sivhybrid和HIV-2。 在过去的一年中，在TNPRC的液氮中存储了1种兔病毒挑战量。 该程序代表核心的扩展，以在TNPRC上包括其他病毒。 RabbitPox是由Chad Roy博士在TNPRC的微生物学部门领导的生物精神研究计划的一部分。