DISPOSITION, METABOLISM & PROCESSING OF OPIATE AGONISTS, ANTAGONISTS & OPIOIDS

性格、新陈代谢

• 批准号: 7722184
• 负责人: MARY J KREEK
• 金额: $ 0.66万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722184
• 关键词: Agonist; Cholecystokinin; Cocaine; Computer Retrieval of Information on Scientific Projects Database; Corticotropin; Corticotropin-Releasing Hormone; Dopamine; Dynorphin A; Dynorphins; Enkephalins; Fluoxetine; Funding; Grant; High Pressure Liquid Chromatography; Hormones; Institution; Mass Spectrum Analysis; Metabolism; Methadone; Methods; Morphine; Narcotic Antagonists; Neuropeptides; Neurotransmitters; Opiates; Opioid Peptide; Parents; Peptides; Pro-Opiomelanocortin; Process; Proteins; Radioimmunoassay; Research; Research Personnel; Resources; Source; Substance P; System; Techniques; United States National Institutes of Health; alcohol effect; beta-Endorphin; drug of abuse; endogenous opioids; extracellular; gamma-MSH; nalmefene; novel; prodynorphin; proenkephalin; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aims are A. To develop novel methods using mass spectrometry to qualitatively (and subsequently, semi-quantitatively or quantitatively) determine the presence of multiple neuropeptides derived from a single gene product (e.g. prodynorphin and processed dynorphin A peptides; proenkephalin and processed enkephalin peptides; proopiomelanocortin (POMC) parent peptide and multiple peptides derived from POMC, adrenocorticotropin hormone (ACTH), beta-endorphin, of gamma-melanocyte-stimulating hormone (MSH). B.To use these novel techniques (alone or in combination with HPLC and/or RIA) for the simultaneous qualitative and/or quantitative analysis of opioid peptides and other neuropeptides related to the endogenous opioid system (e.g. Corticotropin releasing factor (CRF), cholecystokinin (CCK), substance P and their processed peptides). C. To use these techniques in studies of the effects of alterations in levels of activity of neurotransmitters on the processing of endogenous opioids (e.g. the effects of altered levels of dopamine on dynorphin processing). D. To use these techniques to study the effects of drugs of abuse and potential treatment agents on opioid peptide processing and degradation (e.g. cocaine, morphine and ethanol effects; methadone, nalmefene and fluoxetine effects). We are currently developing novel MS tools for determining specific endogenous extracellular neuropeptides.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 具体目的是A.使用质谱法开发新方法，以定性（随后，半定量或定量地）确定源自单个基因产物的多种神经肽的存在（例如，prodynorphin和proded dynorphin and processed dynorphin a肽；肽肽和加工Enkephalin and enkekephalin and eenkephalin and kekephalinin andkephalinin andkephalinin肽； popiomelanocortin（POMC）的肽和来自POMC的多种肽，肾上腺皮质激素激素（ACTH），β-内啡肽，Gamma-melanocyte刺激激素（MSH）。和/或RIA），用于与内源性阿片类药物系统（例如，皮质激素释放因子（CRF），胆固醇（CCK），物质P及其加工肽有关的同时定性和/或定量分析。在研究神经递质活性水平改变对内源性阿片类药物加工的影响的研究中（例如，多巴胺水平改变对测能力的影响）。 D.使用这些技术来研究滥用药物和潜在治疗剂对阿片类肽加工和降解的影响（例如可卡因，吗啡和乙醇效应；美沙酮，纳米芬和氟西汀效应）。 我们目前正在开发用于确定特定内源性细胞外神经肽的新型MS工具。