CO2-INDUCED ANESTHESIA RESULTS IN RAPID INCREASE IN PLASMA LEVELS OF VASOPRESSIN

二氧化碳诱导麻醉导致血浆加压素水平快速升高

• 批准号: 7954140
• 负责人: MARY J KREEK
• 金额: $ 0.24万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954140
• 关键词: Anesthesia procedures; Antibodies; Argipressin; Asphyxia; Blood; Carbon Dioxide; Computer Retrieval of Information on Scientific Projects Database; Corticosterone; Corticotropin; Decapitation; Euthanasia; Exposure to; Funding; Grant; Hormones; Institution; Manuscripts; Measures; Methods; Molecular; Oxytocin; Plasma; Radioimmunoassay; Rattus; Research; Research Personnel; Resources; Rodent; Source; Stress; United States National Institutes of Health; Vasopressins; Work; capillary liquid chromatography; design; immunoreactivity; liquid chromatography mass spectrometry; macromolecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brief anesthesia, such as following exposure to high levels of carbon dioxide, prior to decapitation is considered a more humane alternative for the euthanasia of rodents, compared to use of the decapitation alone. Studies of the levels of certain stress hormones in plasma such as corticosterone and adrenocorticotropic hormone (ACTH) have supported the use of this method of euthanasia in endocrinological and molecular studies. In the current study, rats were briefly exposed to a chamber filled with carbon dioxide until recumbent (20-25 s), and immediately sacrificed via decapitation, and trunk blood collected; findings were compared to rats sacrificed via decapitation with no exposure to carbon dioxide. Radioimmunassays were used to measure arginine vasopressin (AVP) and ACTH immunoreactivity (ir) in plasma. While ACTH-ir levels remained steady following brief exposure to carbon dioxide (in accordance with results of other investigators), AVP-ir levels were increased by more than an order of magnitude. These results were confirmed by quantitative capillary-liquid chromatography-mass spectrometry, indicating this observation of rapid increase in plasma AVP-ir levels is not due to nonspecific recognition by the antibody used in the radioimmunoassay. Likewise, using capillary-liquid chromatography-mass spectrometry, we observed a rapid increase in plasma oxytocin levels following carbon dioxide exposure. These surprising findings have important implications for the design and interpretation of studies involving brief carbon dioxide exposure prior to decapitation, as well as those with euthanasia resulting from carbon dioxide-induced asphyxiation. A manuscript describing this work is currently under review.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 与单独使用斩首相比，短暂的麻醉（例如，在斩首之前暴露于高水平的二氧化碳二氧化碳，被认为是啮齿动物安乐死的更为人道的替代方法。对血浆中某些应激激素水平的研究，例如皮质酮和肾上腺皮质激素激素（ACTH）支持在内分泌学和分子研究中使用这种安乐死方法。在当前的研究中，将大鼠短暂地暴露于充满二氧化碳的腔室，直到卧式（20-25 s），并立即通过斩首牺牲，并收集了躯干血；将发现与通过斩首处死的大鼠进行比较，而没有暴露于二氧化碳。放射免疫测量用于测量血浆中的精氨酸加压素（AVP）和ACTH免疫反应性（IR）。在短暂接触二氧化碳（根据其他研究者的结果）之后，ACTH-IR水平保持稳定，但AVP-IR水平的增加不仅仅是数量级。这些结果通过定量毛细血管色谱 - 质谱法证实，表明血浆AVP-IR水平的快速增加并不是由于放射免疫测定中使用的抗体的非特异性识别所致。同样，使用毛细血管色谱 - 质谱法，我们观察到二氧化碳暴露后血浆催产素水平迅速增加。这些令人惊讶的发现对涉及短暂的二氧化碳暴露的研究和解释具有重要意义，以及二氧化碳诱导的窒息而产生的安乐死的研究。目前正在审查描述这项工作的手稿。