Genotyping, Sequencing and Gene Expression

基因分型、测序和基因表达

• 批准号: 7786018
• 负责人: QING WANG
• 金额: $ 43.81万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7786018
• 关键词: Genotyping; Sequencing; Gene; Expression

The Sequencing, Genotyping and Gene Expression Core will act as a centralized facility to perform large scale and high throughput sequencing, genotyping and microarray analysis for the four projects. Specifically, the Core activities will include: 1) DNA sequence analysis; 2) identification of single nucleotide polymorphisms (SNPs); 3) genotyping of SNPs; and 4) microarray analysis. The advantages for such a centralized facility include: 1) It conserves resources, and is more time and cost efficient; 2) It avoids unnecessary duplication of effort: 3) It standardizes quality control; and 4) It allows for inter-project comparison of results. In addition, the Core will also function as an educational resource to train members of the Program Project laboratories in proper sequencing, genotyping and gene expression methodologies and to provide advice for Project Leaders in the design and implementation of relevant experiments. The training will be valuable for Postdoctoral Fellows and Graduate Students and useful if the demands on the Core exceed its capacity at a particular time. The Sequencing, Genotyping and Gene Expression Core will be housed in the Center for Cardiovascular Genetics in ND40 of the Lemer Research Institute.

测序，基因分型和基因表达核心将充当四个项目的大规模和高吞吐量测序，基因分型和微阵列分析的集中式设施。具体而言，核心活动将包括：1）DNA序列分析； 2）鉴定单核苷酸多态性（SNP）； 3）SNP的基因分型； 4）微阵列分析。这种集中式设施的优势包括：1）它可以保存资源，并且更具时间和成本效益； 2）避免了不必要的努力重复：3）标准化质量控制； 4）它允许对结果进行项目间比较。此外，核心还将充当教育资源，以适当的测序，基因分型和基因表达方法培训计划实验室的成员以及 在设计和实施相关实验的设计和实施中为项目负责人提供建议。该培训对于博士后研究员和研究生将是有价值的，如果核心的需求超过其特定时间的能力，则有用。测序，基因分型和基因表达核心将安置在Lemer Research Institute ND40的心血管遗传学中心中。