Lanthanide Containing ZnS Nanoparticles

含有 ZnS 纳米粒子的镧系元素

• 批准号: 7573743
• 负责人: DAVID H WALDECK
• 金额: $ 21.59万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7573743
• 关键词: Address; Affect; Apoptosis; Biochemical; Biological; Cations; Cells; Cessation of life; Characteristics; Chemicals; Collaborations; Complement; Complex; Coupled; Development; Discrimination; Electron energy loss spectroscopy; Energy Transfer; Environment; Family; Fluorescence; Fluorescence Microscopy; Goals; Image; In Vitro; Investigation; Ions; Lanthanoid Series Elements; Lead; Ligands; Light; Location; Luminescent Measurements; Methodology; Methods; Microscopic; Modeling; Monitor; Nature; Optics; Photons; Physical Chemistry; Plasma; Preparation; Primary Cell Cultures; Property; Publishing; Quantum Dots; Reporter; Research; Resolution; Roentgen Rays; Sampling; Scanning Transmission Electron Microscopy Procedures; Semiconductors; Shapes; Signal Transduction; Simulate; Solutions; Solvents; Spectrum Analysis; Streptavidin; Surface; System; Temperature; Testing; Time; Tissues; Toxic effect; Transmission Electron Microscopy; Water; X ray diffraction analysis; X-Ray Diffraction; absorption; base; biological preparation; biological systems; design; emission spectroscopy; falls; fluorophore; in vivo; luminescence; millisecond; nanocrystal; nanoparticle; novel; optical imaging; practical application; pressure; prevent; programs; public health relevance; quantum; research study; small molecule; ultraviolet; vibration; zinc sulfide

DESCRIPTION (provided by applicant): This proposal describes a program to develop nanoparticles with advanced luminescence properties by combining zinc sulfide (ZnS) semiconductor nanoparticles and luminescent lanthanide cations so that the photophysical advantages of each are exploited. The aim is to use ZnS nanoparticles, with their large absorption cross section, to sensitize the lanthanide cations emission by an "antenna effect" and to provide a matrix that protects the lanthanides from nonradiative deactivation. ZnS is a desirable matrix because it is less toxic than the widely used CdSe nanocrystals, however the ZnS bandgap lies in the ultraviolet/blue spectral region, which has made them incompatible with biological imaging applications because of the strong interference between UV/blue photons and biological systems. However, the addition of lanthanide cations to the ZnS matrix causes the excitation energy to be released through the lanthanide cations as sharp emission bands in the visible and near infrared, determined by the nature of the lanthanide action. The lanthanide cations have sharp well defined emission bands that are insensitive to their environment (such as temperature, pH, pressure or biological environment) and allows for spectral discrimination from biological background (autofluorescence). Lanthanide cations also have longer luminescent lifetimes (micro- to milliseconds) than many other fluorescence emitters, allowing for temporal discrimination between the analyte signal and the background fluorescence. Chemical derivatization of the nanoparticle surface will be used to provide the biochemical selectivity for the nanocrystal probe and to make the material safe and soluble for the targeted biological applications. These materials will be tested in tissue and cell-based preparations and compared to commercially available probes for use in fluorescence microscopy applications. The toxicity of the nanocrystals will be evaluated using highly sensitive optical imaging methodologies for detecting cellular damage and death in primary cell culture models. PUBLIC HEALTH RELEVANCE: We will develop a novel family of luminescent nanoparticles that emit visible or near infrared light and are specifically designed to operate as fluorescent reporters in a broad range of "in vivo" and "in vitro" bioanalytical applications, including biological imaging. Ultimately, these nanoparticles will constitute a versatile luminescence platform whose optical properties will complement existing fluorophores, their signal being easily discriminated from the native fluorescence of biological systems.

描述（由申请人提供）：该提案描述了一个程序，通过将硫化锌（ZNS）半导体纳米颗粒和发光灯笼阳离子结合使用，以开发具有晚期发光特性的纳米颗粒。目的是使用具有较大吸收横截面的ZnS纳米颗粒来通过“天线效应”来敏感灯笼阳离子阳离子的发射，并提供一个保护灯笼的矩阵，以保护灯笼剂免受非辐射式失活。 ZnS是一个理想的矩阵，因为它的毒性比广泛使用的CDSE纳米晶体的毒性小，但是ZnS带隙位于紫外线/蓝色光谱区域，这使其与生物成像应用不相容，因为UV/蓝色光子和生物学系统之间的强烈干扰。然而，向ZnS基质中添加灯笼阳离子会导致通过灯笼阳离子释放激发能，作为可见的和接近红外的尖锐发射带，由兰烷基作用的性质确定。灯笼阳离子具有鲜明定义的发射带，对环境不敏感（例如温度，pH，压力或生物环境），并允许从生物背景（自动荧光）中辨别光谱。与许多其他荧光发射器相比，灯笼阳离子的发光寿命（微秒至毫秒）更长，可以在分析物信号和背景荧光之间进行时间歧视。纳米颗粒表面的化学衍生化将用于为纳米晶探针提供生化选择性，并使材料安全可溶于靶向生物学应用。这些材料将在组织和基于细胞的制剂中进行测试，并将其与用于荧光显微镜应用的市售探针进行比较。将使用高度敏感的光学成像方法来评估纳米晶体的毒性，以检测原代细胞培养模型中的细胞损伤和死亡。 公共卫生相关性：我们将开发一个新型的发光纳米颗粒系列，它们发出可见或近红外光，并专门设计为在广泛的“体内”和“体外”生物分析应用中以荧光记者的作用，包括生物学成像。最终，这些纳米颗粒将构成一个多功能发光平台，其光学特性将补充现有的荧光团，它们的信号很容易与生物系统的天然荧光区分开。