Development of Nanoscale Plasmonic Devices for Creation of a Next Generation Surf

开发纳米级等离激元器件以创造下一代冲浪

基本信息

批准号：
8071591
负责人：
DAVID H WALDECK
金额：
$ 18.17万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-15 至 2013-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8071591
关键词：
Address Area Beryllium Binding Biochemical Phenomena Biological Biological Assay Biological Models Biological Monitoring Biological Process Biological Sciences Biomedical Research Cells Characteristics Chemicals Clinic Coupled Coupling Detection Development Devices Diagnostic Elements Event Film Foundations Generations Glycerol Growth Healthcare Investigation Learning Link Lipids Liquid substance Measurement Measures Medical Membrane Proteins Metabolic Pathway Metabolism Metals Methodology Methods Microfluidic Microchips Microfluidics Miniaturization Modification Monitor Nanoarray Analytical Device Nanotechnology Nature Optics Oxidation-Reduction Pathway interactions Physical Chemistry Play Preparation Process Property Protein Analysis Proteins Regulation Reporting Research Role Side Specificity Spectrum Analysis Structure Surface Surface Plasmon Resonance Technology Thick Time Translations Work analytical tool base biological research charge transfer complex design experience indexing insight instrument light transmission multiplex detection nanometer nanoscale nanostructured new technology next generation photonics plasmonics protein protein interaction public health relevance skills structural biology transmission process

项目摘要

DESCRIPTION (provided by applicant): Recent developments in the design and fabrication of nanometer scale optical devices offer a new technology for the monitoring of biological events. In particular, we propose to exploit these developments to create plasmonic sensing elements and demonstrate their utility for monitoring biological binding events. Because the sensing elements are small, just a few tens of microns on a side, they can be integrated with microfluidic devices to provide a platform for performing parallel and/or multiplex investigations. This technology promises to displace the conventional surface plasmon resonance (SPR) methodology for studying biological interactions, hence its creation and development will have a significant impact on biological and biomedical research. We will demonstrate the capabilities of this new technology by investigating aspects of glycerol metabolism and the specific protein-protein interactions that are essential in its regulation. Through rational design we will prepare specific chemical surface modification and use it to study aspects of this important biological pathway. However, we anticipate that the technology will be broader and by creating plasmonic devices that are easily integrated into microfluidic platforms we will create the fundamental technological foundation that is required to fully exploit plasmonic processes in sensing biological events, leading to a variety of applications in healthcare and life sciences research. This platform will make possible the creation of analytical tools for the preparation, detection, and analysis of proteins that are expressed at low abundance and/or transiently, obtained from biological fluids and cells. PUBLIC HEALTH RELEVANCE (provided by applicant): We are exploiting new developments in photonics and nanotechnology to create a new type of surface plasmon resonance (SPR) spectroscopy. Although conventional SPR is widely used in biomedical research, the proposed technology promises to dramatically grow that use in research and expand its use to medical diagnostics. Because this technology can be integrated with a microfluidic platform we will enable the creation of analytical tools for the preparation, detection, and analysis of proteins that are expressed at low abundance and/or transiently, obtained from biological fluids and cells.

描述（由申请人提供）：纳米尺度光学设备设计和制造方面的最新发展为监测生物事件提供了新技术。特别是，我们建议利用这些发展来创建等离子传感元件，并证明它们用于监测生物结合事件的实用性。由于感应元素很小，因此只有几十微米，因此它们可以与微流体设备集成在一起，以提供平行和/或多重研究的平台。这项技术有望取代用于研究生物学相互作用的常规表面等离子体共振（SPR）方法，因此其创造和开发将对生物学和生物医学研究产生重大影响。我们将通过研究甘油代谢的各个方面以及在调节中必不可少的特定蛋白质蛋白质相互作用来证明这项新技术的能力。通过理性设计，我们将准备特定的化学表面修饰，并使用它来研究这种重要的生物学途径的各个方面。但是，我们预计该技术将会更广泛，并通过创建容易集成到微流体平台中的等离子体设备，我们将创建基本的技术基础，以充分利用等离激元过程在感应生物学事件中，从而在医疗保健和生命科学研究中进行了各种应用。该平台将使创建分析工具，以制备以低丰度和/或瞬时从生物流体和细胞获得的蛋白质制备，检测和分析。公共卫生相关性（由申请人提供）：我们正在利用光子学和纳米技术的新发展来创建一种新型的表面等离子体共振（SPR）光谱。尽管常规SPR广泛用于生物医学研究中，但拟议的技术有望大大发展其在研究中的使用，并将其用于医学诊断。由于该技术可以与微流体平台集成，因此我们将启用分析工具，以制备，检测和分析以低丰度和/或暂时从生物流体和细胞获得的蛋白质。