TRANSGENIC CORE

转基因核心

• 批准号: 7883417
• 负责人: Christi A Walter
• 金额: $ 19万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7883417
• 关键词: Age; Aging; Animals; Binding Sites; Biological Process; Bypass; Cells; Cross-Sectional Studies; Disease; Educational Activities; Embryo; Engineering; Gene Expression; Gene Expression Regulation; Genes; Genomics; Histocompatibility Testing; Individual; Investigation; Laboratory Personnel; Longevity; Mediating; Methods; Mitotic; Molecular; Monitor; Mouse, Founder, Transgenic; Mus; Pathology; Postdoctoral Fellow; Production; Proteins; RNA Interference; Rattus; Regulation; Research Personnel; Rodent; Role; Shock; Students; System; Technology; Testing; Tetracyclines; Tissues; Transgenic Organisms; age related; genetic manipulation; interest; knockout gene; mature animal; new technology; recombinase; tool

The ability to produce defined changes in individual genes through transgenic and gene knockout technologies has provided investigators with powerful tools for understanding the role of specific genes and their corresponding proteins in a variety of biological processes and with the ability to monitor changes in genomic integrity in virtually every cell and tissue type, including post mitotic tissues. Sophisticated methods of regulating gene expression with molecular switches (e.g., tetracycline binary system of gene regulation, Cre recombinase-mediated regulation of sequences flanked by lox P sites) combined with transgenic and gene knockout technologies are facilitating investigations into the roles of specific genes in aging in adult animals by bypassing embryonic expression. Most recently, RNAi has been shown to be a powerful method for studying the role of specific genes and their corresponding proteins and can be used in conjunction with transgenic technology to produce animals in which reduced abundance of specific proteins can be achieved. These genetic manipulations can be used with traditional methods of studying aging (e.g., lifespan studies, cross-sectional pathology studies) to test directly the role of specific genes in aging. The major function of the Transgenic Core is to make genetically manipulated rodents for aging studies. This Core is essential for investigators interested in studying the roles of specific genes in aging and age-related diseases. Accordingly, the Specific Aims of the Transgenic Core are: 1. To produce and identify transgenic founder mice and rats. 2. To assist investigators in the production of mice carrying gene knockouts. 3. To provide educational activities for students, postdoctoral fellows, investigators, and laboratory personnel engaged in using transgenic rodents. 4. To implement new technologies for generating genetically engineered rodents as the need arises.

通过转基因和基因敲除在单个基因中产生明确变化的能力 技术为研究人员提供了强大的工具，用于了解特定基因及其相应蛋白质在各种生物过程中的作用，并能够监测几乎每种细胞和组织类型（包括有丝分裂后组织）的基因组完整性变化。利用分子开关调节基因表达的复杂方法（例如基因调节的四环素二元系统、Cre重组酶介导的lox P位点两侧的序列调节）与转基因和基因敲除技术相结合，正在促进对特定基因在衰老中的作用的研究成人时 动物通过绕过胚胎表达。最近，RNAi已被证明是研究特定基因及其相应蛋白质的作用的有效方法，并且可以与转基因技术结合使用来生产可以降低特定蛋白质丰度的动物。这些基因操作可以与研究衰老的传统方法（例如寿命研究、横断面病理学研究）结合使用，以直接测试特定基因在衰老中的作用。转基因核心的主要功能是制造用于衰老研究的基因操纵啮齿动物。对于有兴趣研究特定基因在衰老和年龄相关疾病中的作用的研究人员来说，这一核心至关重要。因此，转基因核心的具体目标是： 1. 产生并鉴定转基因创始小鼠和大鼠。 2. 协助研究人员制作携带基因敲除的小鼠。 3. 为从事转基因啮齿动物使用的学生、博士后、研究人员和实验室人员提供教育活动。 4. 根据需要实施新技术来产生基因工程啮齿动物。