TRANSGENIC CORE

转基因核心

• 批准号: 6946249
• 负责人: Christi A Walter
• 金额: $ 10万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6946249
• 关键词: aging; biomedical facility; developmental genetics; gene targeting; genetically modified animals; health care personnel education; laboratory mouse; laboratory rat; technology /technique development; transfection /expression vector

The ability to produce defined changes in individual genes through transgenic and gene knockout technologies has provided investigators with powerful tools for understanding the role of specific genes and their corresponding proteins in a variety of biological processes and with the ability to monitor changes in genomic integrity in virtually every cell and tissue type, including post mitotic tissues. Sophisticated methods of regulating gene expression with molecular switches (e.g., tetracycline binary system of gene regulation, Cre recombinase-mediated regulation of sequences flanked by lox P sites) combined with transgenic and gene knockout technologies are facilitating investigations into the roles of specific genes in aging in adult animals by bypassing embryonic expression. Most recently, RNAi has been shown to be a powerful method for studying the role of specific genes and their corresponding proteins and can be used in conjunction with transgenic technology to produce animals in which reduced abundance of specific proteins can be achieved. These genetic manipulations can be used with traditional methods of studying aging (e.g., lifespan studies, cross-sectional pathology studies) to test directly the role of specific genes in aging. The major function of the Transgenic Core is to make genetically manipulated rodents for aging studies. This Core is essential for investigators interested in studying the roles of specific genes in aging and age-related diseases. Accordingly, the Specific Aims of the Transgenic Core are: 1. To produce and identify transgenic founder mice and rats. 2. To assist investigators in the production of mice carrying gene knockouts. 3. To provide educational activities for students, postdoctoral fellows, investigators, and laboratory personnel engaged in using transgenic rodents. 4. To implement new technologies for generating genetically engineered rodents as the need arises.

通过转基因和基因敲除产生单个基因的定义变化的能力 技术为研究人员提供了强大的工具，以了解特定基因及其相应蛋白质在各种生物学过程中的作用，并能够监测几乎每种细胞和组织类型的基因组完整性变化，包括丝分裂组织。用分子开关调节基因表达的复杂方法（例如，基因调节的四环素二进制二元系统，CRE重组酶介导的调节侧面lox p位点的序列的调节）与转基因和基因基因敲除技术结合了成年特异性基因在成年特异性基因中的研究促进 动物通过绕过胚胎表达。最近，RNAi已被证明是研究特定基因及其相应蛋白质的作用的强大方法，并且可以与转基因技术结合使用，以生产动物，从而可以实现降低特定蛋白质的丰度。这些遗传操作可以与传统的研究衰老方法一起使用（例如寿命研究，横断面病理研究），以直接测试特定基因在衰老中的作用。转基因核心的主要功能是使遗传操纵的啮齿动物进行衰老研究。该核心对于有兴趣研究特定基因在衰老和年龄相关疾病中的作用的研究人员至关重要。因此，转基因核心的具体目的是：1。产生和识别转基因创始人小鼠和大鼠。 2。协助研究人员的生产携带基因敲除的小鼠。 3。为使用转基因啮齿动物的学生提供教育活动，博士后研究员，调查人员和实验室人员。 4。实施新技术，以产生基因工程的啮齿动物。