Cell Biology Core

细胞生物学核心

• 批准号: 7924709
• 负责人: Larry R. Gerace
• 金额: $ 42.13万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7924709
• 关键词: Arts; Behavior; Biological Assay; Cells; Cellular Structures; Cellular biology; Collaborations; Complex; Complex Mixtures; Computer Simulation; Confocal Microscopy; Electron Microscope; Image; In Vitro; Life; Light; Multiprotein Complexes; Nuclear Lamina; Nucleocytoplasmic Transport Proteins; Nucleoproteins; Protein Analysis; Proteomics; Research Infrastructure; Research Institute; Screening procedure; Technical Expertise; Techniques; Technology; Time; base; in vivo; inhibitor/antagonist; instrumentation; light microscopy; macromolecule; nucleocytoplasmic transport; protein complex; small molecule; structural biology; trafficking

Overview The Cell Biology and Imaging Core provides access to state-of-the-art facilities and technologies for light and electron microscope imaging of macromolecules in fixed and living cells, proteomic analysis of complex mixtures of proteins, and analysis of cell structure and function by various in vitro and in vivo techniques. Thematic focus and technical expertise includes nucleocytoplasmic transport of proteins and nucleoprotein complexes, dynamics of multiprotein complexes of cells such as the nuclear lamina, and screening for small molecule inhibitors of nuclear transport using cell-based assays. The infrastructure of the center includes collaborations with Drs. Gaudenz Danuser and John Yates at the Scripps Research Institute. Gaudenz Danuser is a leading expert in mathematical analysis and computational modeling of the dynamic behavior of cellular components, and provides access to instrumentation for spinning disc confocal microscopy for quantitative real-time light microscopy. John Yates provides access to highthroughput proteomics for analysis of complex protein fractions.

概述 细胞生物学和成像核心可访问最先进的设施和光的技术 固定细胞和活细胞中大分子的电子显微镜成像，蛋白质组学分析 蛋白质的复杂混合物，以及各种体外和体内对细胞结构和功能的分析 技术。主题重点和技术专长包括蛋白质的核质运输和 核蛋白复合物，核薄片等细胞多蛋白复合物的动力学，以及 使用基于细胞的测定法对核转运的小分子抑制剂进行筛选。基础设施 该中心包括与DRS的合作。 Gaudenz Danuser和John Yates在Scripps Research 研究所。 Gaudenz Danuser是数学分析和计算建模的领先专家 细胞成分的动态行为，并提供旋转盘的仪器的访问 共聚焦显微镜用于定量实时光学显微镜。约翰·耶茨（John Yates）提供了对高发射的访问 蛋白质组学用于分析复杂蛋白质分数。