Core--Molecular Biology

核心--分子生物学

• 批准号: 7798097
• 负责人: Jason P Affourtit
• 金额: $ 9.54万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7798097
• 关键词: DNA; DNA Resequencing; Evolution; Gene Expression; Genome; Genotype; Laboratories; Maps; Molecular Biology; Molecular Biology Techniques; Procedures; RNA; SNP genotyping; Sampling; Services; Time; Tissues; research study

The Molecular Biology Core will support the CGD aims that require the use of molecular biology techniques on a large scale. The Core will provide the following support: Project 1) prepare and track DNA samples for resequencing and genotyping; Project by Churchill) isolate RNA from tissues, prepare and analyze microarrays, and validate the results of the microarray experiments using quantitative real-time PCR; and Project by Petkov) isolate DNA, followed by SNP genotyping and mapping. The procedures for Project by Churchill will be carried out in The Jackson Laboratory's Gene Expression Service (see R&E), and the genotyping for Project by Petkov will be carried out at KBiosciences (Hoddeston, Herts, UK).

分子生物学核心将支持需要使用分子生物学的 CGD 目标 大规模的技术。核心将提供以下支持： 项目 1) 准备和追踪 DNA 用于重新测序和基因分型的样本； Churchill 的项目）从组织中分离 RNA、制备和分析 微阵列，并使用定量实时 PCR 验证微阵列实验的结果；和 Petkov 的项目）分离 DNA，然后进行 SNP 基因分型和作图。 Churchill 项目的程序将在杰克逊实验室的基因表达服务（参见 R&E）中进行，Petkov 项目的基因分型将在 KBiosciences（英国赫茨霍德斯顿）进行。