200keV, Energy Filtered, Intermediate-High Voltage Transmission Electron Microscope(IVEM)"

200keV、能量过滤、中高压透射电子显微镜(IVEM)"

• 批准号: 10642585
• 负责人: Mark H Ellisman
• 金额: $ 200万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-10 至 2024-09-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10642585
• 关键词: 20 year old; 3-Dimensional; Adoption; Cells; Communities; Custom; Data Storage and Retrieval; Democracy; Electron Microscope; Electron Microscopy; Electrons; Environment; Funding; Goals; High Performance Computing; Image; Infrastructure; Label; Methods; Microscope; Microscopic; Microscopy; Preparation; Production; Research; Research Personnel; Sampling; Specific qualifier value; Specimen; Technology; Thick; Tissues; Transmission Electron Microscopy; United States National Institutes of Health; Visualization; computerized data processing; cost; imaging modality; instrument; method development; novel; supercomputer; tomography; tool; transmission process; voltage

PROJECT SUMMARY / ABSTRACT This application outlines our request for funding to procure a new 200keV, energy filtered, intermediate-high voltage transmission electron microscope (IVEM) (Thermo-Fisher/FEI Talos F200C G2). This instrument will be completely unique to the La Jolla, CA research Mesa (and it’s many research organizations) and bring into production use cutting edge (and complementary) methods for energy filter enabled, multiple label transmission electron microscopy (e.g., ColorEM) and high throughput 3D electron microscopic tomography (3D EM) of semi- thick epoxy-embedded samples of cells and tissues (non-Cryo). Procurement of this microscope will address two pressing needs: 1) it will directly support growing demand for these imaging methods/technologies by both local and Nationally distributed NIH investigators, currently users of the National Center for Microscopy and Imaging Research (NCMIR); and 2) it will provide production access to cutting-edge methods previously only available on high-end, specialized platforms built to enable development of methods like ColorEM, (fulfilling a goal of NIH to lower barriers to the use and to promote broader dissemination and impact). As such, it will also propel the democratization of these key technologies. The specified new instrument will be procured at a significantly lower cost, compared to the instrument it will replace, a 20 year old custom-built JEOL 3200 IVEM, soon not maintainable by JEOL. The new tool also simplifies installation, with more modest room requirements, allowing adoption of the methods it enables by more NIH researchers. The platform will be automated and set- up for easy access by the community and be supported by a team of experts in electron microscopy, specimen preparation and data processing, analysis, and visualization. Its environment will also include (and make direct use) of novel high-performance computing and data storage infrastructure of the San Diego Supercomputer Center and its affiliated National Research Platform (NRP).

项目概要/摘要 该申请概述了我们为采购新的 200keV、能量过滤、中高电压而需要的资金请求 电压透射电子显微镜 (IVEM) (Thermo-Fisher/FEI Talos F200C G2)。 加利福尼亚州拉霍亚梅萨研究中心（以及它的许多研究组织）完全独特，并将其带入 生产使用尖端（和补充）方法来实现能量过滤器启用、多标签传输 半电子显微镜（例如 ColorEM）和高通量 3D 电子显微镜断层扫描（3D EM） 厚厚的环氧树脂包埋细胞和组织样品（非冷冻）的采购将解决。 两个紧迫的需求：1）它将直接支持双方对这些成像方法/技术不断增长的需求 地方和全国分布的 NIH 研究人员，目前是国家显微镜中心的用户 成像研究 (NCMIR)；2) 它将提供以前仅提供的尖端方法的生产途径 可在高端、专门的平台上使用，该平台旨在支持 ColorEM 等方法的开发（满足 NIH 的目标是降低使用障碍并促进更广泛的传播和影响）。 推动这些关键技术的民主化。 与它将取代的已有 20 年历史的定制 JEOL 3200 IVEM 仪器相比，成本显着降低， JEOL 很快就无法维护新工具还简化了安装，对空间的要求更适中， 允许更多 NIH 研究人员采用该平台所支持的方法，该平台将实现自动化和设置。 以便社区轻松访问，并得到电子显微镜、标本专家团队的支持 其环境还将包括（并进行直接的）准备和数据处理、分析和可视化。 使用）圣地亚哥超级计算机的新型高性能计算和数据存储基础设施 中心及其附属国家研究平台（NRP）。