Microbial interactions in otitis media

中耳炎中的微生物相互作用

• 批准号: 7644662
• 负责人: WILLIAM E SWORDS
• 金额: $ 31.04万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7644662
• 关键词: Accounting; Address; Affect; Antibiotic Resistance; Antibiotic Therapy; Antibiotics; Bacteria; Childhood; Chinchilla (genus); Chronic; Clinical; Communities; Data; Diagnosis; Disease; Environment; Haemophilus influenzae; Immune response; Immunity; In Vitro; Incidence; Individual; Infection; Inflammatory Response; Knowledge; Learning; Liquid substance; Microbial Biofilms; Modeling; Moraxella (Branhamella) catarrhalis; Office Visits; Operative Surgical Procedures; Organism; Otitis Media; Outcome; Pathogenesis; Patients; Population; Prevalence; Public Health; Recurrence; Resistance; Sampling; Severities; Severity of illness; Streptococcus pneumoniae; Treatment Failure; Tube; Tympanostomy; base; clinical practice; clinically relevant; economic impact; improved; in vivo; insight; microorganism interaction; middle ear; novel; novel strategies; pathogen; patient population; prevent; public health relevance; response

DESCRIPTION (provided by applicant): This project aims to define how polymicrobial infection influences bacterial otitis media. Haemophilus influenzae (Hi), Streptococcus pneumoniae (Sp), and Moraxella catarrhalis (Mcat) are the predominant isolates from patients with OM, and a wealth of evidence indicates that OM often involves combinations of these organisms. We hypothesize that interactions between bacterial species can impact OM disease parameters that include bacterial persistence, resistance to clearance and antibiotic treatment, and the initiation, progression and severity of the host inflammatory response. In order to address this hypothesis we will complete the following Specific Aims: Specific Aim 1: To define interactions among bacterial pathogens causing OM. Specific Aim 2: To ask how different interspecies relationships affect experimental OM. Specific Aim 3: To evaluate impact of polymicrobial infection on antibiotic resistance. Specific Aim 4: To establish the incidence of coinfection in patients undergoing tympanostomy. Most of our current knowledge about bacterial pathogenesis is derived from infection studies using pure cultures that may not fully represent how infections occur in actual patients. This proposal presents an opportunity to learn how multiple pathogens inhabiting the same ecological niche influence one another in biofilm communities that are critical to persistent infections. PUBLIC HEALTH RELEVANCE: Otitis media is a major common and costly pediatric illness worldwide, accounting for billions of dollars per year in total economic impact. Otitis media infections are the leading reason for pediatric office visits, new antibiotic prescriptions, and surgical instillation of tympanic drain tubes to relieve chronic and recurrent otitis media is the most commonly performed surgical procedure in the U.S. Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis have long been recognized as the most common bacterial causes of otitis media, and it is now clear that many of these infections involve simultaneous infection with more than one organism. The focus of this application is to understand how these bacterial species interact in the biofilm communities that are a major determinant of bacterial persistence in vivo. Understanding how bacteria form these biofilms will be an important step in learning to better diagnose, prevent, and/or treat chronic infections.

描述（由申请人提供）：该项目旨在定义多数菌感染如何影响细菌性中炎培养基。流感嗜血杆菌（HI），肺炎链球菌（SP）和Cartarrhalis（MCAT）是来自OM患者的主要分离株，大量证据表明OM通常涉及这些生物体的组合。我们假设细菌物种之间的相互作用会影响OM疾病参数，包括细菌持久性，对清除和抗生素治疗的抵抗力以及宿主炎症反应的起始，进展和严重程度。为了解决这一假设，我们将完成以下特定目的：特定目的1：定义引起OM的细菌病原体之间的相互作用。特定目的2：询问不同种间关系如何影响实验OM。特定目的3：评估多数菌感染对抗生素耐药性的影响。特定目的4：确定接受鼓膜术的患者共同感染的发生率。我们目前对细菌发病机理的大多数知识是使用纯培养物来得出的，这些纯培养物可能无法完全代表实际患者的感染方式。该提案为了解居住在同一生态生态位的多种病原体如何在生物膜群落中彼此影响的多种病原体提供了机会，这对于持续感染至关重要。公共卫生相关性：中耳炎是全球主要常见且昂贵的儿科疾病，每年的经济影响总计数十亿美元。 Otitis media infections are the leading reason for pediatric office visits, new antibiotic prescriptions, and surgical instillation of tympanic drain tubes to relieve chronic and recurrent otitis media is the most commonly performed surgical procedure in the U.S. Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis have long been recognized as the most common bacterial中耳炎的原因，现在很明显，其中许多感染涉及与多个生物体同时感染。该应用的重点是了解这些细菌物种如何在生物膜群落中相互作用，这是体内细菌持久性的主要决定因素。了解细菌如何形成这些生物膜将是学习更好地诊断，预防和/或治疗慢性感染的重要步骤。