CB: Tissue Core

CB：组织核心

• 批准号: 7507324
• 负责人: Rafael Fonseca
• 金额: $ 12.69万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7507324
• 关键词: Address; Agreement; Archives; Biological; Blood specimen; Bone Marrow; Cell Separation; Cells; Clinic; Clinical; Clinical Data; Collaborations; Collection; Compatible; Core Facility; Cryopreservation; DNA; Dana-Farber Cancer Institute; Data; Databases; Diagnosis; Eastern Cooperative Oncology Group; Ensure; Funding; Future; Goals; Individual; Institution; Investigation; Laboratories; Lead; Measurement; Molecular; Molecular Genetics; Multiple Myeloma; Patients; Principal Investigator; Procedures; Process; RNA; Reproduction spores; Research; Research Personnel; Research Project Grants; Resistance; Resources; Sampling; Science; Serum; Slide; Specific qualifier value; Specimen; Standards of Weights and Measures; Testing; Time; Tissues; Transport Process; data management; design; experience; follow-up; outcome forecast; programs; research study; response; sample collection; statistics; tumor

The goals of the Tjssue Bank Core are to aid SPORE investigators: 1) to identify tumor biologic and molecular genetic correlates of diagnosis, prognosis, response, progression, and survival in the context of the SPORE related investigations ; 2) to maintain a searchable database of clinical and laboratory data for use by SPORE investigators; and 3) to provide a resource for banked specimens for future studies. Core Laboratory functions are designed to increase the power of the individual projects to detect biologic differences among patients entering the SPORE studies and identify molecular correlates of response versus resistance, and to be flexible enough to address individual investigator needs. In the previous funding period, the tissue core was located at the well established Mayo Clinic Myeloma Core facilities which received, processed, stored, archived and distributed clinically annotated cellular (bone marrow and PB cells) and molecular (DMA and RNA) and serum samples to investigators participating in this SPORE. Samples from the SPORE have been collected, tested, and archived separately from other Core resources, such as the ECOG or the Mayo Program Project reference laboratories; however, these resources have been shared between these cores resources, thereby increasing the pool of samples available for correlative science by the SPORE investigators. Due to the increased needs of our growing SPORE and in close cooperation and collaboration with the core at the Mayo clinic, we have now established a tissue core facility at the Dana Farber Cancer Institute. To make efforts at the Mayo Clinic and at the DFCI seamless and compatible for annotated sample sharing, we have utilized the same standard operating procedures for bone marrow processing, myeloma cell purification, and cryopreservation. Our biostatical and clinical data management at both the institutions are integrated in Core 3, which allows sharing of data and compatibility in sample selection for research studies. This Core provides an independent, current, and quickly searchable database of clinical and laboratory results and archived biospecimens. Data forms completed at the time of collection of the samples are entered into a common clinical, laboratory, and archival database. Follow up data is obtained and entered as required. All data are then stored in a common database accessible by the Statistics center. All patients have measurement of key biological variables requested by SPORE investigators. Unused specimen including sorted cells, DNA, RNA and cytospin slides are stored for future use on all patients. Subsequent use of banked samples beyond that specified in this SPORE proposal will be provided only with agreement from SPORE Director and the SPORE Principal Investigators. This Core is lead by Drs. Fonseca and Munshi, who are experienced in managing Core laboratories in a multi-institutional cooperative setting.

TJSSUE银行核心的目标是帮助孢子调查人员：1）确定肿瘤生物学和 在诊断，预后，反应，进展和生存中的分子遗传相关性 孢子相关的研究； 2）维护可搜索的临床和实验室数据数据库 孢子调查人员使用； 3）为将来的研究提供资源。核 实验室功能旨在增加单个项目检测生物学的力量 进入孢子研究的患者之间的差异并确定了反应的分子相关性 相对于抵抗力，并且足够灵活以满足个人研究人员的需求。在上一个 资金期，组织核心位于良好的梅奥诊所骨髓瘤核心设施中 接收，处理，存储，存档和分布临床注释的细胞（骨髓和PB 细胞）和分子（DMA和RNA）以及参与该孢子的研究者的血清样品。 从孢子中收集，测试和存档的样本与其他核心资源分开， 例如ECOG或Mayo计划项目参考实验室；但是，这些资源有 在这些核心资源之间共享，从而增加了可用于相关性的样本池 孢子调查人员的科学。由于我们不断增长的孢子和密切合作的需求增加了 并与梅奥诊所的核心合作，我们现在已经建立了一个组织核心设施 达娜·法伯癌症研究所。在Mayo诊所和DFCI无缝和 兼容带注释的样品共享，我们使用了相同的骨头标准操作程序 骨髓加工，骨髓瘤细胞纯化和冷冻保存。我们的生物统计和临床数据 这两个机构的管理都集成到核心3中，该机构允许共享数据和兼容性 在研究研究中的样本选择中。该核心提供了一个独立，当前和快速搜索的 临床和实验室结果的数据库以及存档的生物测量。数据表格在 样品的收集被输入到一个常见的临床，实验室和档案数据库中。跟进 根据需要获得并输入数据。然后将所有数据存储在可访问的常见数据库中 统计中心。所有患者均测量孢子要求的关键生物变量 调查人员。未使用的标本，包括排序的细胞，DNA，RNA和细胞质载体，以备将来 用于所有患者。随后使用本孢子提案中指定的银行样本将 仅由孢子主管和孢子首席调查员达成协议。这个核心是 由博士领导。 Fonseca和Munshi，他们在管理多机构的核心实验室方面经验丰富 合作环境。