TECHNICAL CORE

技术核心

基本信息

批准号：
7646481
负责人：
DAVID JONATHAN BRENNER
金额：
$ 25.22万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7646481
关键词：
3-Dimensional Academic Medical Centers Accounting Address Alpha Particle Emitter Alpha Particles Antitumor Drug Screening Assays Applications Grants Area Award Biological Biological Models Biological Process Biology Bystander Effect Caliber Calibration Cartoons Categories Cell Count Cell Line Cells Charge Cluster Analysis Code Collaborations Communities Computer Workstations Computer software Condition Confidence Intervals Conflict (Psychology)Cytogenetics Daily Data Data Analyses Data Set Databases Daughter Deposition Development Devices Dimensions Dose Electrostatics Elements End Point Energy-Generating Resources Engineering Ensure Experimental Designs Frequencies Funding Gait Gene Expression Generations Genes Genetic Genomic Instability Glass Goals Grant Graph Half-Life Heart Hour Housekeeping Gene Hybrids Illinois Imagery Imaging Techniques Individual Informatics Investigation Ionizing radiation Ions Laboratories Lasers Lens (device)Life Link Logic Magnetism Maintenance Markov Chains Measurement Measures Mediating Mediator of activation protein Microscopic Microscopy Modeling Molecular Biology Motivation Mutation National Center for Research Resources Nature Noise Nuclear Numbers Odds Ratio Oncogenes Ontology Operative Surgical Procedures Optics Particle Accelerators Pathway interactions Pattern Peer Review Phase Physics Placement Plant Roots Plumbing Power Sources Principal Investigator Probability Procedures Process Property Protons PubMed Publications Purpose Radiation Radiation-Induced Gene Expression Radioactive Radioisotopes Range Relative (related person)Reporting Research Research Personnel Resolution Resources Risk Running Sampling Schedule Series Side Signal Transduction Signaling Molecule Solutions Source Special Populations Networks Speed Spottings Staging Staining method Stains Standards of Weights and Measures Statistical Data Interpretation Statistical Methods Stochastic Processes Structure System Systems Biology Techniques Testing Time TimeLine Tissues Touch sensation Trees Triplet Multiple Birth United States National Institutes of Health Universities Update Voice Week Work Writing base beamline biological adaptation to stress cellular targeting data acquisition data mining day design desire detector distributed data dosimetry experience experimental analysis graphical user interface improved insight instrument instrumentation interest ion source irradiation lens micronucleus monolayer particle particle beam programs protocol development research study response simulation size submicron tool vector

项目摘要

DESCRIPTION (provided by applicant): The overall objectives of this program project application are to define and characterize the mechanisms of the bystander effect and to compare and contrast the nature of the signaling molecule(s) for the bystander effect versus genomic instability. Three well linked, fully integrated projects are proposed to address these issues and will be supported by the functions of the Technical Core. The main goals of the Technical Core include 1) Provide statistical and data analyses support to all the projects to ensure that uniform statistical methods will be used in all studies. 2) Provide experimental design support based on Monte Carlo simulation of input parameters from the three projects of this program project, to improve and optimize data acquisition. 3) Apply inverse modeling approaches to analyze the gene expression data generated in the projects, to provide insight into the underlying regulatory networks. 4) Provide expertise in the design and fabrication of special irradiation fixtures through the Instrument shop (Machine Shop) in our Center. 5) Provide support with respect to the daily operation of the accelerator based single-cell/ single-particle microbeam and also the stand-alone single-cell/ single-particle microbeam. Through collaborations with the Project Leaders of the individual projects, the Technical Core will facilitate the coordination of research efforts of this program project by a combination of experimental, theoretical and computational approaches.

描述（由申请人提供）：该计划项目应用程序的总体目标是定义和表征旁观者效应的机制，并比较和对比旁观者效应与基因组不稳定性的信号分子的性质。提出了三个链接完好的项目来解决这些问题，并将得到技术核心功能的支持。技术核心的主要目标包括1）为所有项目提供统计和数据分析支持，以确保在所有研究中使用统一的统计方法。 2）基于该计划项目三个项目的输入参数的蒙特卡洛模拟提供实验设计支持，以改进和优化数据采集。 3）应用逆建模方法分析项目中产生的基因表达数据，以洞悉潜在的监管网络。 4）通过我们中心的仪表店（机械车间）设计和制造特殊辐照装置的专业知识。 5）提供有关基于加速器的单细胞/单粒子微束的日常操作的支持独立的单细胞/单粒子微束。通过与各个项目的项目负责人的合作，技术核心将通过实验，理论和计算方法的结合来促进该计划项目的研究工作。