Infrared Laser Desorption Ion Mobility Mass Spectrometer

红外激光解吸离子淌度质谱仪

基本信息

批准号：
7664534
负责人：
John Albert Schultz
金额：
$ 19.27万
依托单位：
IONWERKS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-07-10 至 2010-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7664534
关键词：
Acceleration Aplysia Bacteria Biological Biological Markers Biomedical Research Brain Cell Mobility Cerebrospinal Fluid Charge Chemicals Clinical Collaborations Collection Communities Complex Computer software Data Detection Development Diagnostic Disease Drug Addiction Engineering Funding Generations Goals Grant Height Image Ions Laboratory Rat Lasers Legal patent Lipids Louisiana Maps Mass Spectrum Analysis Mental disorders Methods Modeling National Institute of Drug Abuse Nature Neuropeptides Noise Peptides Performance Phase Physiologic pulse Positioning Attribute Postdoctoral Fellow Problem Solving Process Proteins Proteomics Pulse Rates Pump Rattus Research Research Personnel Resolution Sampling Signal Transduction Slice Solutions Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Students System Techniques Technology Testing Time Tissues United States National Institutes of Health Universities Wood material Work analog base brain tissue commercial application complex biological systems data acquisition detector digital drug of abuse env Gene Products graduate student improved insight instrument interest ion mobility ionization mass spectrometer novel programs small molecule tool trend two-dimensional ultraviolet voltage

项目摘要

DESCRIPTION (provided by applicant): The development of an infrared laser desorption ionization ion mobility mass spectrometer (IR LDI IM MS) will be continued. The analysis of test biomolecules such as peptides and proteins in the Phase I of the project has demonstrated that the new system's performance is not only comparable, but, in some aspects, superior to the UV MALDI mode due to both mobility pre-separation of ion and softer nature of the IR MALDI. These results could provide a breakthrough in both top down proteomics as well as protein biomarker identification. Matrix-free laser desorption as well as efficient two-dimensional analysis of rat brain tissues and bacteria was also demonstrated. Based on the proof of capabilities of the technique in the Phase I, the instrument will be applied to the analysis of biological tissues. Both matrix-assisted and matrix-free approach will be used. The instrument will be upgraded in order to further increase the throughput, sensitivity and resolution. As part of this research, Ionwerks is collaborating with the mass spectrometry research group of Prof. Kermit Murray at Louisiana State University who are the experts in the use of IR lasers for the direct analysis of biomolecules. The utility of the IR LDI IM MS system spatial imaging of drugs of abuse, metabolite, lipids, and neuropeptides on and within rat brain tissue sections and the model Aplysia californica will be demonstrated. An advanced technique of the analysis of complex biological systems will be developed, which will have significant advantages compared to the conventional UV MALDI. Important commercial applications of this technology are in biomedical research and clinical diagnostics.

描述（申请人提供）：红外激光解吸电离离子淌度质谱仪（IR LDI IM MS）的开发将继续进行。该项目一期对肽和蛋白质等测试生物分子的分析表明，新系统的性能不仅具有可比性，而且在某些方面优于 UV MALDI 模式，因为离子的迁移率预分离IR MALDI 的性质更柔和。这些结果可以为自上而下的蛋白质组学和蛋白质生物标志物鉴定提供突破。还展示了无基质激光解吸以及对大鼠脑组织和细菌的有效二维分析。基于第一阶段技术能力的证明，该仪器将应用于生物组织的分析。将使用基质辅助和无基质方法。该仪器将进行升级，以进一步提高通量、灵敏度和分辨率。作为这项研究的一部分，Ionwerks 正在与路易斯安那州立大学 Kermit Murray 教授的质谱研究小组合作，他们是使用红外激光直接分析生物分子的专家。将展示 IR LDI IM MS 系统对大鼠脑组织切片上和内部的滥用药物、代谢物、脂质和神经肽以及加州海兔模型进行空间成像的实用性。将开发一种分析复杂生物系统的先进技术，与传统的UV MALDI相比将具有显着的优势。该技术的重要商业应用是生物医学研究和临床诊断。