Biomarker Quantitation by NP-MS Tissue Imaging.

通过 NP-MS 组织成像进行生物标志物定量。

基本信息

批准号：
8592448
负责人：
John Albert Schultz
金额：
$ 95.72万
依托单位：
IONWERKS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-06-01 至 2016-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8592448
关键词：
Affect Animal Disease Models Animals Biological Biological Markers Biopolymers Blood Carbon Chemistry Collaborations Computer software Data Data Analyses Data Set Detection Disease Dose Figs - dietary Gases Goals Growth Helium Hydrogen Image Imagery Implant Injury Ions Laboratories Lipids Location Manufacturer Name Marketing Measurement Measures Microscope Modeling Molecular National Institute of Drug Abuse Nitrogen Paper Particle Size Pharmaceutical Preparations Phase Preclinical Drug Evaluation Preclinical Testing Problem Solving Procedures Protocols documentation Publishing Rattus Relative (related person)Reproducibility Resolution Resource Development Rice Rodent Rodent Diseases Rodent Model Sampling Severities Silver Software Tools Solutions Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Structure Structure of molecular layer of cerebellar cortex Sulfoglycosphingolipids Surface System Techniques Technology Testing Time Tissue Sample Tissues Universities Variant Work brain tissue disorder control drug discovery implantation improved injured instrument interest ion mobility molecular imaging nanoparticulate nanoscale new growth palliative plasmonics pre-clinical prototype public health relevance screening software development tool

项目摘要

DESCRIPTION (provided by applicant): MALDI tissue imaging, when enabled by Nanoparticulate (NP) Matrix implantation, is maturing into a useful preclinical tool for biomarker and drug discovery in our laboratory and at NIDA-IRP. Quantitatively reproducible images from serial coronal sections suggest that relative quantitation is now possible in rodent models of disease and injury. Nanoparticulate (NP) matrix implantation allows numerical quantitation of differences in biomarker levels in rodent disease and injury models. No commercial instrument which performs mass spectral molecular imaging of tissues has software capable of analyzing and displaying these quality data sets. 1) Software and hardware developed under our antecedent phase II for analyzing the multidimensional data from our MALDI-Ion Mobility-MS imaging system will be expanded to serve this need and will also have dual use in correlating molecular image quality with the nano-scale characterization of the NP matrices by advance materials analysis techniques such as the subnanometer resolution Zeiss Orion Helium Ion Microscope. 2) The continued growth of unique core shell NP matrices will continue to be aided by advanced materials analysis measurement of the NP structures and particle size distributions after implantation into the tissue. 3) Protocols for standards incorporation into and onto the tissue surface will attempt to prove the capability of relative and absolute quantitation in MALDI-MS imaging.

描述（由申请人提供）：MALDI 组织成像，当通过纳米颗粒 (NP) 基质植入实现时，正在成熟为我们实验室和 NIDA-IRP 的生物标志物和药物发现的有用临床前工具。来自连续冠状切片的定量可重复图像表明，在疾病和损伤的啮齿动物模型中现在可以进行相对定量。纳米颗粒（NP）基质植入可以对啮齿动物疾病和损伤模型中生物标志物水平的差异进行数值定量。执行组织质谱分子成像的商业仪器没有能够分析和显示这些质量数据集的软件。 1) 我们之前的第二阶段开发的用于分析来自我们的 MALDI-离子淌度-MS 成像系统的多维数据的软件和硬件将得到扩展以满足这一需求，并且还将在将分子图像质量与纳米级表征相关联方面具有双重用途通过先进的材料分析技术（例如亚纳米分辨率 Zeiss Orion 氦离子显微镜）对 NP 矩阵进行分析。 2) 植入组织后，对纳米粒子结构和粒度分布进行先进材料分析测量，将继续有助于独特核壳纳米粒子基质的持续生长。 3) 将标准纳入组织表面的方案将尝试证明 MALDI-MS 成像中相对和绝对定量的能力。