ANIMAL IMAGING

动物成像

基本信息

批准号：
7671810
负责人：
JASON Arthur KOUTCHER
金额：
$ 13.64万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-07-31 至 2012-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7671810
关键词：
Animals Area Arts Autoradiography Bioluminescence Capital Detection Development Disease Disease regression Disseminated Malignant Neoplasm Effector Cell Equipment Evaluation Film Floor Fluorescence Fluorescent Probes Gene Expression Genetic Housing Human Hypoxia Image Imaging Techniques Immune Improve Access Incidence Intervention Invasive Knock-out Laboratory Research Localized Longitudinal Studies Magnetic Resonance Magnetic Resonance Imaging Magnetic Resonance Spectroscopy Malignant Neoplasms Memorial Sloan-Kettering Cancer Center Metabolic Microtome - medical device Modality Modeling Modernization Monitor Numbers Optical Tomography Optics Patients Pharmacodynamics Phase Photons Positron Process Radioisotopes Radiolabeled Reporter Genes Research Research Infrastructure Research Personnel Resolution Rodent Rodent Model Screening procedure Services Spectrum Analysis Staging Stem cells System Testing Tracer Transgenic Organisms Tumor Biology Ultrasonography Validation X-Ray Computed Tomography Xenograft Model base biosecurity cancer cell digital disease natural history fluorescence microscope host neoplasm interaction in vivo insight instrumentation molecular imaging neoplastic cell optical imaging radiotracer single photon emission computed tomography spectroscopic imaging tomography trafficking tumor tumor initiation

项目摘要

The Animal Imaging Core provides services for molecular imaging of rodent models of human cancer. The Core provides investigators with non-invasive, high-resolution quantitative imaging-based capabilities for metabolic and genetic characterization of tumors and their microenvironment, including in vivo trafficking of tumor cells. This is accomplished by monitoring of "directly-targeting" probes and of the expression of single and multi-modality reporter genes using optical (bioluminescence and fluorescence), radionuclide (PET, SPECT, and autoradiography), MRI/MRS, CT, and US imaging. These imaging techniques are wellestablished at MSKCC for small-animal imaging studies. They are quantitative and non-invasive and thus readily adaptable to longitudinal studies. The Animal Imaging Core also provides investigators with access to critical ancillary equipment and services such as a Fuji Film BAS-180011 phosphor-plate digital autoradiography system, a digital-camera-equipped Olympus B 201 fluorescence microscope with motorized stage, and a Microm HM500M cryostatic microtome. As part of a major expansion and modernization of MSKCC's infrastructure (Phase 1), part of the Imaging Core has recently re-located to the C4 level within the Vivarium of the new Zuckerman Research Center. This area provides enhanced biosecurity and functionality and houses our R4 and Focus 120 microPET dedicated high-resolution small-animal (rodent) PETs, new MS 200 optical imaging system, microCAT II dedicated small-animal (rodent) CT, and X-SPECT dedicated small-animal SPECT-CT. The existing MS 100 optical imaging system and recently installed Vevo 770 ultrasound system have been re-located to refurbished space on the 12th floor of the Rockefeller Research Laboratory providing improved access to the primary users of these two systems. The Imaging Core's 4.7-T 40-cm bore and 7.0-T 31-cm Brucker NMR imaging and spectroscopy systems are currently located in the MRI Building. In Phase 2 of MSKCC's capital expansion and modernization, all of the foregoing instrumentation of the Imaging Core will be consolidated in spacious, state-of the-art quarters with the Center's vivarium.

动物成像核心为人类癌症啮齿动物模型的分子成像提供服务。核心为研究人员提供非侵入性、高分辨率定量成像功能用于肿瘤及其微环境的代谢和遗传特征，包括体内运输肿瘤细胞。这是通过监测“直接靶向”探针和使用光学（生物发光和荧光）、放射性核素的单模态和多模态报告基因（PET、SPECT 和放射自显影）、MRI/MRS、CT 和 US 成像。这些成像技术已经很成熟在 MSKCC 从事小动物成像研究。它们是定量的且非侵入性的，因此易于适应纵向研究。动物成像核心还为研究人员提供了访问权限关键的辅助设备和服务，例如 Fuji Film BAS-180011 荧光板数字放射自显影系统，配备数码相机的 Olympus B 201 荧光显微镜，电动载物台和 Microm HM500M 低温切片机。作为 MSKCC 基础设施重大扩建和现代化（第一阶段）的一部分，成像 Core 最近搬迁至新祖克曼研究中心动物园内的 C4 层。该区域提供增强的生物安全性和功能性，并容纳我们的 R4 和 Focus 120 microPET 专用高分辨率小动物（啮齿动物）PET、新型 MS 200 光学成像系统、microCAT II 专用小动物（啮齿动物）CT、X-SPECT专用小动物SPECT-CT。现有的MS 100 光学成像系统和最近安装的 Vevo 770 超声系统已搬迁至洛克菲勒研究实验室 12 楼的空间经过翻新，可更方便地访问这两个系统的主要用户。成像核心的 4.7-T 40-cm 孔径和 7.0-T 31-cm Brucker 核磁共振成像和光谱系统目前位于核磁共振大楼。在 MSKCC 的第二阶段资本扩张和现代化，成像核心的所有上述仪器都将中心的动物园位于宽敞、先进的宿舍内。