Molecular Biology Shared Resource

分子生物学共享资源

• 批准号: 7698816
• 负责人: SHIRLEY M TAYLOR
• 金额: $ 1.02万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-03 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7698816
• 关键词: Animals; Biochemistry; Biology; Cancer Center; Characteristics; Chemicals; Chemistry; Client; Cloning; Collaborations; Communities; Complementary DNA; Consultations; Core Facility; Custom; Daily; Endotoxins; Epitopes; Equipment and supply inventories; Event; Facility Construction Funding Category; Faculty; Feedback; Funding; Gene Targeting; Generations; Genomic Library; Grant; Head; Human Resources; Individual; Insecta; Knock-out; Knockout Mice; Laboratories; Laboratory Personnel; Laboratory Technicians; Libraries; Maintenance; Malignant Neoplasms; Mammalian Cell; Maps; Methods; Mission; Molecular; Molecular Analysis; Molecular Biology Shared Resource; Occupations; Peer Review; Phase; Plasmid Cloning Vector; Plasmids; Polymerase Chain Reaction; Postdoctoral Fellow; Procedures; Production; Proteins; Published Comment; Quality Control; Range; Recording of previous events; Refractory; Research; Research Personnel; Research Project Grants; Research Support; Resource Sharing; Resources; Respondent; Screening procedure; Services; Site; Students; Support of Research; Surveys; System; Techniques; Therapeutic; Time; Training; Transgenic Organisms; Update; Variant; Vendor; Virus; Work; base; cost; design; design and construction; embryonic stem cell; experience; expression vector; homologous recombination; medical specialties; member; new technology; programs; protein expression; response; scale up; user-friendly; vector

The construction and manipulation of plasmids and of cloned DMA sequences is a central activity of every laboratory involved in the molecular analysis of cancer. Some manipulations are more challenging and some plasmids refractory to common procedures. The Molecular Biology Shared Resource was initiated to assist Cancer Center investigators with plasmid manipulations and cloning projects that prove problematic to individual laboratories or that prove refractory to manipulation by laboratory technicians, students, and post-docs. In addition, the Molecular Biology Shared Resource provides a cost-effective alternative to a variety of basic services to the investigators of the Massey Cancer Center (MCC). These basic services include endotoxin-free plasmid purification, insert purification, plasmid management and maintaining an inventory of plasmid vectors and clones. Other projects are designed upon the specific needs of client laboratories including custom subcloning, library screening, generation and optimization of protein expression vectors, design, construction, and analysis of gene targeting vectors, and protein production in bacterial, insect and mammalian cells. The MBSR works in synch with the Transgenic and Knockout Core Facility and facilitates access of Cancer Center members to such animals through vector design and construction. A large part of the activity of the Core involves plasmid manipulations that have proven difficult for the requesting laboratory, so each job tends to have unique characteristics and usually involves trouble-shooting and redesign of procedures. Thus, the expertise of Facility personnel has proven to be one of the most valuable services offered by the Core. MSBR personnel offer training seminars and previews of newer technologies as they are developed, and often train laboratory personnel in routine (and not so routine) molecular procedures. The Core Director offers a formal course in all phases of molecular technique that can be taken for credit or audited. New directions for the Molecular Biology Shared Resource are continuously being sought, in consultation with Cancer Center investigators and the MBSR oversight committee. The recent hire of new faculty in Chemical Biology into the Departments of Chemistry and Biochemistry (members of the Molecular Cancer Therapeutics program) has promoted a move towards the support of protein production for structural studies. The Shared Resource has been increasingly active during the past 2 years assisting investigators with their protein production needs, generating expression vectors and optimizing protein expression systems for scaled-up production.

质粒和克隆 DMA 序列的构建和操作是核心活动 每个参与癌症分子分析的实验室。有些操作更具挑战性 以及一些对常规程序难以处理的质粒。分子生物学共享资源是 发起协助癌症中心研究人员进行质粒操作和克隆项目，证明 对个别实验室有问题或证明实验室技术人员难以操纵， 学生和博士后。此外，分子生物学共享资源提供了具有成本效益的 为梅西癌症中心 (MCC) 的研究人员提供各种基本服务的替代方案。这些 基本服务包括无内毒素质粒纯化、插入片段纯化、质粒管理和 维护质粒载体和克隆的库存。其他项目根据具体情况设计 客户实验室的需求，包括定制亚克隆、文库筛选、生成和优化 蛋白质表达载体、基因打靶载体和蛋白质的设计、构建和分析 在细菌、昆虫和哺乳动物细胞中生产。 MBSR 与转基因和 淘汰核心设施并促进癌症中心成员通过载体接触此类动物 设计和施工。核心的大部分活动涉及质粒操作 事实证明，对于提出要求的实验室来说是困难的，因此每项工作往往都有独特的特点，并且通常 涉及故障排除和程序重新设计。因此，设施人员的专业知识已得到证明 成为核心提供的最有价值的服务之一。 MSBR 人员提供培训研讨会和 预览新技术的开发，并经常对实验室人员进行日常培训（以及 不那么常规）分子程序。核心主任提供分子生物学各个阶段​​的正式课程 可以取得学分或经过审计的技术。 人们不断寻求分子生物学共享资源的新方向， 与癌症中心研究人员和 MBSR 监督委员会协商。最近聘用的新人 化学生物学系的教师进入化学系和生物化学系（分子生物学系成员） 癌症治疗计划）促进了结构性蛋白质生产的支持 研究。在过去的两年里，共享资源越来越活跃地协助调查人员 根据他们的蛋白质生产需求，生成表达载体并优化蛋白质表达 扩大生产规模的系统。