Microarray Core B

微阵列核心B

基本信息

批准号：
7846854
负责人：
Shawn Edward Levy
金额：
$ 8.61万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-06-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7846854
关键词：
Academic Medical Centers Bioinformatics Cancer Center Candidate Disease Gene Collection Complementary DNA Core Facility DNA DNA analysis Data Data Quality Data Set Diabetes Mellitus Diagnosis Digestive System Disorders Disease Ensure Equipment Experimental Designs Gene Expression Gene Expression Profiling Gene Targeting Genes Genetic Polymorphism Genome Genome Mappings Genomics Genotype Goals Human Individual Informatics Investigation Investments Knowledge Laboratories Manuscripts Maps Messenger RNA Methodology MicroRNAs Microarray Shared Resource Molecular Molecular Profiling Mus Oligonucleotide Microarrays Process Publishing RNA Interference Research Research Personnel Research Training Resources Reverse Transcriptase Polymerase Chain Reaction Sampling Services Spottings Support System Techniques Technology Time Translating Variant base cost density design expression vector follow-up functional genomics gene function improved interest mammalian genome meetings prevent programs research study structural genomics tool web interface

项目摘要

The large-scale analysis of gene expression profiles was the first hallmark in the transition from structural genomics to functional genomics. Genomic-scale expression profiling experiments require large technical and computational investments to facilitate both experimentation and the analysis of the large data sets produced. These investments are beyond the capabilities of most laboratories or small groups. To provide access to these important technologies, the DMA Microarray Core, operating within the Vanderbilt Microarray Shared Resource (VMSR) has been established with major support from the VDDRC, the Diabetes Research and Training Center, the Vanderbilt-lngram Cancer Center (VICC), and the Vanderbilt University Medical Center Office of Research. The objectives of this core are to assist investigators in performing genomic investigations using high-throughput and cutting-edge technologies that are financially or technically prohibitive to utilize in individual labs. The services in the core have been substantially increased since the last application. While previous services were limited to cDNA-based arrays produced in the core for mouse and human, current services include a variety of commercial array platforms in addition to comprehensive oligonucleotide arrays for mouse and human. Supported commercial arrays include Affymetrix, Applied Biosystems, and CodeLink. In addition to gene expression profiling services, the core offers high-throughput genotyping services using the Affymetrix mapping arrays and Targeted Genotyping (ParAllele) platforms. Verification of microarray results (expression and genotyping) by real-time PCR is also available. The newest services established in the core are profiling of microRNAs and the manipulation of gene expression via RNAi and cDNA over-expression to support functional screens. The core continues to maintain a comprehensive informatics program with two full-time analysts available. Significantly, the core has developed an on-line project management system that supports all sample submission, project annotation, and data access through an efficient web interface. Since 2002, the core has published several manuscripts, processed hundreds of samples for VDDRC investigators, and continued to expand and refine services to meet the research needs of the Vanderbilt DDRC. The Core provides investigators with cutting edge access to human genomic technologies in order to prevent, diagnose or improve treatment of digestive diseases and disorders.

基因表达谱的大规模分析是从结构分析转变的第一个标志。基因组学到功能基因组学。基因组规模的表达谱实验需要大量的技术和计算投资以促进大数据集的实验和分析产生的。这些投资超出了大多数实验室或小团体的能力。提供访问这些重要技术，即在范德比尔特微阵列内运行的 DMA 微阵列核心共享资源 (VMSR) 的建立得到了 VDDRC、糖尿病协会的大力支持研究和培训中心、范德比尔特-英格拉姆癌症中心 (VICC) 和范德比尔特大学医学中心研究办公室。该核心的目标是协助调查人员执行使用高通量和尖端技术进行基因组研究，这些技术在经济或技术上禁止在个别实验室中使用。自成立以来，核心服务已大幅增加最后一次申请。虽然之前的服务仅限于在小鼠核心中生产的基于 cDNA 的阵列和人力，目前的服务除了全面的服务外，还包括各种商业阵列平台用于小鼠和人类的寡核苷酸阵列。支持的商业阵列包括 Affymetrix、Applied 生物系统和 CodeLink。除了基因表达谱服务外，该核心还提供高通量使用 Affymetrix 作图芯片和靶向基因分型 (ParAllele) 平台的基因分型服务。还可以通过实时 PCR 验证微阵列结果（表达和基因分型）。这核心中建立的最新服务是 microRNA 分析和基因表达操作通过 RNAi 和 cDNA 过表达来支持功能筛选。核心继续保持综合信息学计划，配有两名全职分析师。值得注意的是，该核心具有开发了在线项目管理系统，支持所有样品提交、项目注释、以及通过高效的网络界面进行数据访问。自2002年以来，核心已发布了多个手稿，为 VDDRC 调查人员处理了数百个样本，并不断扩展和完善服务以满足范德比尔特 DDRC 的研究需求。该核心为研究人员提供了最先进的人类基因组技术，以便预防、诊断或改善消化系统疾病和紊乱的治疗。