Microarray Core B

微阵列核心B

• 批准号: 7283910
• 负责人: Shawn Edward Levy
• 金额: $ 8.34万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7283910
• 关键词: Academic Medical Centers; Bioinformatics; Cancer Center; Candidate Disease Gene; Collection; Complementary DNA; Core Facility; DNA; DNA analysis; Data; Data Quality; Data Set; Diabetes Mellitus; Diagnosis; Digestive System Disorders; Disease; Ensure; Equipment; Experimental Designs; Gene Expression; Gene Expression Profiling; Gene Targeting; Genes; Genetic Polymorphism; Genome; Genome Mappings; Genomics; Genotype; Goals; Human; Individual; Informatics; Investigation; Investments; Knowledge; Laboratories; Manuscripts; Maps; Messenger RNA; Methodology; MicroRNAs; Microarray Shared Resource; Molecular Profiling; Mus; Oligonucleotide Microarrays; Polymerase Chain Reaction; Process; Publishing; RNA Interference; Research; Research Personnel; Research Training; Resources; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Services; Spottings; Support System; Techniques; Technology; Time; Translating; Variant; base; cost; density; design; expression vector; follow-up; functional genomics; gene function; improved; interest; mammalian genome; prevent; programs; research study; structural genomics; tool; web interface

The large-scale analysis of gene expression profiles was the first hallmark in the transition from structural genomics to functional genomics. Genomic-scale expression profiling experiments require large technical and computational investments to facilitate both experimentation and the analysis of the large data sets produced. These investments are beyond the capabilities of most laboratories or small groups. To provide access to these important technologies, the DMA Microarray Core, operating within the Vanderbilt Microarray Shared Resource (VMSR) has been established with major support from the VDDRC, the Diabetes Research and Training Center, the Vanderbilt-lngram Cancer Center (VICC), and the Vanderbilt University Medical Center Office of Research. The objectives of this core are to assist investigators in performing genomic investigations using high-throughput and cutting-edge technologies that are financially or technically prohibitive to utilize in individual labs. The services in the core have been substantially increased since the last application. While previous services were limited to cDNA-based arrays produced in the core for mouse and human, current services include a variety of commercial array platforms in addition to comprehensive oligonucleotide arrays for mouse and human. Supported commercial arrays include Affymetrix, Applied Biosystems, and CodeLink. In addition to gene expression profiling services, the core offers high-throughput genotyping services using the Affymetrix mapping arrays and Targeted Genotyping (ParAllele) platforms. Verification of microarray results (expression and genotyping) by real-time PCR is also available. The newest services established in the core are profiling of microRNAs and the manipulation of gene expression via RNAi and cDNA over-expression to support functional screens. The core continues to maintain a comprehensive informatics program with two full-time analysts available. Significantly, the core has developed an on-line project management system that supports all sample submission, project annotation, and data access through an efficient web interface. Since 2002, the core has published several manuscripts, processed hundreds of samples for VDDRC investigators, and continued to expand and refine services to meet the research needs of the Vanderbilt DDRC. The Core provides investigators with cutting edge access to human genomic technologies in order to prevent, diagnose or improve treatment of digestive diseases and disorders.

基因表达谱的大规模分析是从结构的过渡的第一个标志 功能基因组学的基因组学。基因组尺度表达分析实验需要大量技术 和计算投资，以促进实验和分析大数据集 生产。这些投资超出了大多数实验室或小组的能力。提供 访问这些重要技术，即DMA微阵列核心，在范德比尔特微阵列中运行 共享资源（VMSR）是在VDDRC（糖尿病）的主要支持下建立的 研究与培训中心，Vanderbilt-Lngram癌症中心（VICC）和范德比尔特大学 医疗中心研究办公室。该核心的目标是协助调查人员执行 使用高通量和尖端技术在财务上或技术上都是基因组研究 在单个实验室中使用的过敏性。自从 最后一个应用。虽然先前的服务仅限于鼠标核心产生的基于cDNA的阵列 当前的服务包括各种商业阵列平台，除了全面 小鼠和人类的寡核苷酸阵列。支持的商业阵列包括Affymetrix，应用 生物系统和Codelink。除了基因表达分析服务外，核心还提供高通量 使用Affymetrix映射阵列和靶向基因分型（Parallele）平台的基因分型服务。 还提供了实时PCR的微阵列结果（表达和基因分型）的验证。这 核心建立的最新服务是microRNA的分析和基因表达的操纵 通过RNAi和cDNA过表达来支持功能屏幕。核心继续保持 综合信息计划，可提供两位全职分析师。重要的是，核心具有 开发了一个在线项目管理系统，该系统支持所有样本提交，项目注释， 以及通过有效的Web界面访问数据。自2002年以来，核心已经发布了几篇 手稿，为VDDRC调查人员处理了数百个样本，并继续扩展和完善 满足Vanderbilt DDRC的研究需求的服务。 核心为研究人员提供了对人类基因组技术的最先进的机会 预防，诊断或改善对消化系统疾病和疾病的治疗。