Development of DNA-templated IR quantum dots

DNA 模板红外量子点的开发

基本信息

批准号：
7618247
负责人：
Shana O Kelley
金额：
$ 10.8万
依托单位：
UNIVERSITY OF TORONTO
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-05-01 至 2011-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7618247
关键词：
Address Binding Biological Biological Assay Biological Markers Biology Biosensor Caliber Cell Culture Techniques Cells Cellular Assay Chemicals Chemistry Coupling DNA DNA Sequence Detection Development Diagnosis Diagnostic Imaging Disease Engineering Environment Exhibits Generations Glutamate Carboxypeptidase II Growth In Vitro Kidney Libraries Life Malignant Neoplasms Medical Medicine Methods Modification Monitor Nucleic Acids Nucleotides Oligonucleotides Optics Organic solvent product Organism Penetration Performance Plasma Production Property Prostate-Specific Antigen Quantum Dots Reporting Research Infrastructure Resources Route Semiconductors Solutions Specificity Staging Structure of molecular layer of cerebellar cortex Surface Techniques Time Toxic effect Vertebral column aptamer aqueous base cancer diagnosis cellular imaging combinatorial design fluorophore improved in vivo luminescence nanocrystal nanomaterials nanoparticle nanoscale novel novel strategies particle programs quantum tumor

项目摘要

DESCRIPTION (provided by applicant): Functionalized semiconductor quantum dots have previously been demonstrated to bind to markers on cells. Through their spectrally-narrow optical emissions, they illuminate tumors and other harbingers of disease. They are enabling the highly specific detection of a range of diseases at the earliest stages. We propose to pursue a new and improved class of semiconductor quantum dots. We have shown already that, by seeding the growth of nanoparticles using a DNA template, we are able to produce quantum dots that are efficient, stable emitters in the infrared wavelengths (so-called biological window) in which living organisms are much more transparent than in the visible wavelengths, and in which living organisms' autofluorescence is orders of magnitude lower than in the visible. These materials retain their luminescence properties over time even in biological media such as plasma at 37o C. The collaborating team of Dr. Shana Kelley, a nucleic acids chemist, and Dr. Edward Sargent, an optoelectronics engineer, bring the expertise required to optimize the DNA-grown nanoparticles for applications in medical diagnosis. The strategy of directing the growth of luminescent nanoparticles using DNA provides a one-pot route towards the strong coupling of light-emitting tags with DNA-based aptamers for programmable specific-binding. The project is divided into the following Specific Aims: 1) Direct the growth, and thereby maximize the performance, of DNA-templated quantum dots using designer DNA sequences; 2) Discover optimized DNA sequences for specific binding assays using in vitro selection. We will thereby develop new means of creating highly luminescent nanomaterials for medicine and biology. The team, with its complementary expertise in biomolecular chemistry and optical materials, is equipped with the infrastructure and resources to make a significant contribution to the realization of improved visible and infrared fluorophores for diagnosis.

描述（由申请人提供）：以前已证明功能化的半导体量子点与细胞上的标记结合。通过光谱光学排放，它们照亮了肿瘤和其他疾病先驱。他们在最早的阶段可以高度特定地检测一系列疾病。我们建议追求新的和改进的半导体量子点。我们已经表明，通过使用DNA模板播种纳米颗粒的生长，我们能够产生在红外波长（所谓的生物窗口）中具有有效，稳定的发射器的量子点，在这种量子上，生物的生物体比可见的生物具有比在生物体的自动化效果高的降量范围相比，在这种量子上比可见的波长更透明。这些材料即使在37oC的血浆等生物学培养基中也会保留其发光特性。核酸化学家Shana Kelley博士的合作团队和光电工程师的Edward Sargent博士带来了优化用于医疗诊断的DNA成年纳米颗粒所需的专业知识。使用DNA指导发光纳米颗粒生长的策略为通过基于DNA的适体的强烈发光标签的强耦合提供了一台途径，以进行可编程的特定结合。该项目分为以下特定目的：1）使用设计器DNA序列指导生长，从而最大程度地提高DNA-量子点的性能； 2）发现使用体外选择的特定结合测定的优化DNA序列。因此，我们将开发新的方法来为医学和生物学创建高度发光的纳米材料。该团队及其在生物分子化学和光学材料方面具有互补的专业知识，配备了基础设施和资源，可以为改善可见和红外荧光团的改善做出重大贡献。