Development of DNA-templated IR quantum dots

DNA 模板红外量子点的开发

基本信息

批准号：
7618247
负责人：
Shana O Kelley
金额：
$ 10.8万
依托单位：
UNIVERSITY OF TORONTO
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-05-01 至 2011-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7618247
关键词：
Address Binding Biological Biological Assay Biological Markers Biology Biosensor Caliber Cell Culture Techniques Cells Cellular Assay Chemicals Chemistry Coupling DNA DNA Sequence Detection Development Diagnosis Diagnostic Imaging Disease Engineering Environment Exhibits Generations Glutamate Carboxypeptidase II Growth In Vitro Kidney Libraries Life Malignant Neoplasms Medical Medicine Methods Modification Monitor Nucleic Acids Nucleotides Oligonucleotides Optics Organic solvent product Organism Penetration Performance Plasma Production Property Prostate-Specific Antigen Quantum Dots Reporting Research Infrastructure Resources Route Semiconductors Solutions Specificity Staging Structure of molecular layer of cerebellar cortex Surface Techniques Time Toxic effect Vertebral column aptamer aqueous base cancer diagnosis cellular imaging combinatorial design fluorophore improved in vivo luminescence nanocrystal nanomaterials nanoparticle nanoscale novel novel strategies particle programs quantum tumor

项目摘要

DESCRIPTION (provided by applicant): Functionalized semiconductor quantum dots have previously been demonstrated to bind to markers on cells. Through their spectrally-narrow optical emissions, they illuminate tumors and other harbingers of disease. They are enabling the highly specific detection of a range of diseases at the earliest stages. We propose to pursue a new and improved class of semiconductor quantum dots. We have shown already that, by seeding the growth of nanoparticles using a DNA template, we are able to produce quantum dots that are efficient, stable emitters in the infrared wavelengths (so-called biological window) in which living organisms are much more transparent than in the visible wavelengths, and in which living organisms' autofluorescence is orders of magnitude lower than in the visible. These materials retain their luminescence properties over time even in biological media such as plasma at 37o C. The collaborating team of Dr. Shana Kelley, a nucleic acids chemist, and Dr. Edward Sargent, an optoelectronics engineer, bring the expertise required to optimize the DNA-grown nanoparticles for applications in medical diagnosis. The strategy of directing the growth of luminescent nanoparticles using DNA provides a one-pot route towards the strong coupling of light-emitting tags with DNA-based aptamers for programmable specific-binding. The project is divided into the following Specific Aims: 1) Direct the growth, and thereby maximize the performance, of DNA-templated quantum dots using designer DNA sequences; 2) Discover optimized DNA sequences for specific binding assays using in vitro selection. We will thereby develop new means of creating highly luminescent nanomaterials for medicine and biology. The team, with its complementary expertise in biomolecular chemistry and optical materials, is equipped with the infrastructure and resources to make a significant contribution to the realization of improved visible and infrared fluorophores for diagnosis.

描述（由申请人提供）：功能化半导体量子点先前已被证明可以与细胞上的标记物结合。通过其光谱窄的光发射，它们照亮了肿瘤和其他疾病的先兆。它们能够在最早阶段对一系列疾病进行高度特异性的检测。我们建议开发一种新的、改进的半导体量子点。我们已经证明，通过使用 DNA 模板播种纳米粒子的生长，我们能够生产出在红外波长（所谓的生物窗口）内有效、稳定的发射器的量子点，其中活体比生物体透明得多在可见光波长范围内，生物体的自发荧光比可见光波长低几个数量级。即使在 37o C 的等离子体等生物介质中，这些材料也会随着时间的推移保留其发光特性。核酸化学家 Shana Kelley 博士和光电工程师 Edward Sargent 博士的合作团队带来了优化发光材料所需的专业知识。 DNA 生长的纳米颗粒在医学诊断中的应用。使用 DNA 引导发光纳米粒子生长的策略提供了一种一锅法途径，使发光标签与基于 DNA 的适体强耦合，以实现可编程的特异性结合。该项目分为以下具体目标： 1) 使用设计者 DNA 序列指导 DNA 模板量子点的生长，从而最大限度地提高其性能； 2) 使用体外选择发现用于特异性结合测定的优化 DNA 序列。因此，我们将开发新的方法来制造用于医学和生物学的高发光纳米材料。该团队凭借在生物分子化学和光学材料方面的互补专业知识，配备了基础设施和资源，为实现改进的可见光和红外荧光团诊断做出了重大贡献。