Nanoscale Electrocatalytic Protein Detection

纳米级电催化蛋白质检测

• 批准号: 6913033
• 负责人: Shana O Kelley
• 金额: $ 15.45万
• 依托单位: BOSTON COLLEGE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-21 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6913033
• 关键词: DNA binding protein; DNA repair; biomarker; biotechnology; carbonates; electrochemistry; electrodes; high throughput technology; nanotechnology; oligonucleotides; prostate neoplasms; prostate specific antigen; protein quantitation /detection; synthetic nucleotide; technology /technique development

DESCRIPTION (provided by applicant): This proposal focuses on a novel system for the electrochemical detection of cancer related protein targets using a nanoscale electrode platform. The assay proposed relies on an electro catalytic process involving two transition-metal ions that reports on biomolecular complexation events. Because the reporter system responds to changes in the electrostatics of an electrode surface, it will enable the analysis of nucleic acids/protein and peptide/protein complexes. The proposed project has 3 specific aims: 1) electro catalytic detection of DNA repair proteins implicated in cancer using DNA-modified electrodes; 2) optimization of electro catalytic detection and multiplexed analysis of cellular DNA repair activities, and 3) development of a generalized electro catalytic protein detection method using prostate cancer biomarkers. The electro catalytic protein detection system described is advantageous because it will allow the analysis and discrimination of multiprotein complexes in addition to uncomplexed analytes and will have high selectivity and specificity. Additionally, the protein detection assays will be conducted using nanoelectrodes that will allow multiplexed detection of panels of different biomolecular targets.

描述（由申请人提供）： 该提案重点关注一种使用纳米级电极平台电化学检测癌症相关蛋白质靶标的新型系统。所提出的测定方法依赖于涉及两种过渡金属离子的电催化过程，该过程报告生物分子络合事件。由于报告系统会对电极表面静电的变化做出响应，因此它将能够分析核酸/蛋白质和肽/蛋白质复合物。该项目有 3 个具体目标：1）使用 DNA 修饰电极电催化检测与癌症有关的 DNA 修复蛋白； 2) 优化电催化检测和细胞 DNA 修复活性的多重分析，以及 3) 开发使用前列腺癌生物标志物的通用电催化蛋白检测方法。所描述的电催化蛋白质检测系统是有利的，因为除了未复合的分析物之外，它还允许分析和区分多蛋白质复合物，并且具有高选择性和特异性。此外，蛋白质检测分析将使用纳米电极进行，这将允许对不同生物分子靶标的组进行多重检测。