MRI GUIDED PHOTOIMMUNOTHERAPY

MRI引导光免疫治疗

• 批准号: 7610255
• 负责人: WEI R CHEN
• 金额: $ 6.96万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610255
• 关键词: Animal Experiments; Animals; Biological; Chickens; Computer Retrieval of Information on Scientific Projects Database; Funding; Future; Gel; Grant; Image; Imaging Techniques; Imiquimod; Immune response; Immunity; Immunization; Immunologic Adjuvants; Immunotherapy; In Situ; Institution; Lasers; Long-Term Effects; Lung Adenocarcinoma; Lung Neoplasms; Magnetic Resonance Imaging; Mammary Neoplasms; Mammary gland; Methods; Modality; Monitor; Mus; Neoplasm Metastasis; Patients; Photochemotherapy; Phototherapy; Porfimer Sodium; Rate; Research; Research Personnel; Resources; Solutions; Source; Structure; Temoporfin; Temperature; Testing; Tissues; United States National Institutes of Health; animal tissue; base; glycated chitosan; irradiation; melanoma; novel; response; sarcoma; tumor; tumor growth

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Laser immunotherapy is a novel modality, which utilizes the direct tumor destruction by selective phototherapies and the indirect, long-term effect of immunological stimulation, for the treatment of metastatic tumors. The combination of photoirradiation and in situ applications of immunoadjuvants has induced tumor-specific immunity in previous animals studies. The overall objective of this project is to optimize the modality and to understand its biological mechanism. Methods: Magnetic Resonance Imaging (MRI) technique was used to monitor selective laser thermal treatment of phantom gel, chicken tissue and mouse tumors in order to test the hypothesis of temperature control in laser treatment. Two novel immunoadjuvants, glycated chitosan (GC) and imiquimod (IMQ), were intralesionally injected to mice bearing B16 melanoma and the responses of the animals were studied. To further study the effect of the combination of laser of immunoadjuvant, photodynamic therapy (PDT) and glycated chitosan were used in the treatment of EMT6 mammary tumors and Line 1 lung tumors in mice. Results: The MRI images of the target tissue and animal tumors during laser irradiation showed clear tissue structure changes and temperature distribution. Animal experiments showed that when the GC and IMQ were used together, the tumor growth was significantly slowed. In the treatment of EMT6 mammary sarcoma in mice, GC of 0.5% and 1.5% concentrations increased the cure rates of Photofrin-based PDT treatment from 38% to 63% and 75%, respectively. In the treatment of Line 1 lung adenocarcinoma in mice, a 1.67% GC solution enabled a non-curative mTHPC-based PDT to cure 37% of the tumor bearing mice. Conclusion: Following the current studies, it is expected that the control of tissue temperature during laser irradiation can be achieved, making the optimization of thermal treatment possible. Future study will also demonstrate the enhancement of immune responses in tumor treatment using the combination of laser and immunoadjuvants. Ultimately, this method could be applied clinically for the patients with metastatic tumors.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 简介：激光免疫疗法是一种新型的方式，它利用选择性光疗法的直接肿瘤破坏以及免疫学刺激的间接，长期影响，用于治疗转移性肿瘤。 在以前的动物研究中，光辐射和免疫辅助的原位应用诱导了肿瘤特异性免疫。 该项目的总体目的是优化方式并了解其生物学机制。 方法：使用磁共振成像（MRI）技术来监测幻影凝胶，鸡肉组织和小鼠肿瘤的选择性激光处理，以测试激光治疗中温度控制的假设。 两种新型的免疫助剂，糖化壳聚糖（GC）和咪喹莫德（Imq）（IMQ），是对携带B16黑色素瘤的小鼠的内部注射，并研究了动物的反应。 为了进一步研究免疫辅助，光动力疗法（PDT）和糖化壳聚糖的激光组合的作用，用于治疗小鼠EMT6乳腺肿瘤和1号肺肿瘤。 结果：激光照射期间目标组织和动物肿瘤的MRI图像显示清晰的组织结构变化和温度分布。 动物实验表明，当一起使用GC和IMQ时，肿瘤生长显着减慢。 在小鼠中EMT6乳腺肉瘤的治疗中，GC的GC浓度为0.5％和1.5％，将基于光的PDT治疗的治愈率分别从38％到63％和75％。 在小鼠中1肺腺癌1肺腺癌的治疗中，1.67％的GC溶液使非抑制性MTHPC的PDT可以治愈37％的含有肿瘤轴承小鼠的PDT。 结论：经过当前的研究，预计可以在激光照射期间控制组织温度，从而使热处理的优化成为可能。 未来的研究还将证明使用激光和免疫辅助药的组合在肿瘤治疗中增强免疫反应。 最终，该方法可以在临床上用于转移性肿瘤患者。