A Comprehensive 5K Plus Glycan Microarray

综合 5K Plus 聚糖微阵列

• 批准号: 10353411
• 负责人: Lei Li
• 金额: $ 51.92万
• 依托单位: Z BIOTECH, LLC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10353411
• 关键词: Address; Affinity; Avidity; Binding; Biological; Biological Markers; Biotechnology; Businesses; Cell surface; Cells; Characteristics; Collection; Complex; Computer software; Custom; Data Analyses; Data Scientist; Data Set; Databases; Development; Disease; Engineering; Ensure; Enzymes; Event; Financial cost; Fostering; Funding; Glycobiology; Glycopeptides; Grant; Image; Immune system; Immunotherapy; Industrialization; Intuition; Lectin; Libraries; Liquid substance; Mannose; Marketing; Mediating; Methods; Mind; Mucins; Pharmacologic Substance; Phase; Physiological; Polysaccharides; Positioning Attribute; Process; Production; Proteins; Provider; Public Health; Quality Control; Research; Research Institute; Research Personnel; Resources; Route; Scientist; Signal Transduction; Small Business Innovation Research Grant; Source; Specific qualifier value; Specificity; Spottings; Structure; Sulfate; Surface; Systems Integration; Therapeutic; Time; Universities; Vaccines; Vendor; analysis pipeline; automated analysis; base; commercial application; comparative; cost; experience; insight; macromolecule; manufacturing scale-up; molecular recognition; new technology; programs; receptor; research and development; scale up; sugar nucleotide; surface coating; tool

Project Summary / Abstract The Glycan Array has proven a valuable tool in the study of glycans. Comparatively the Glycan Array gives the greatest view of glycan binding specificity for the lowest cost in terms of analytical turn-around time and financial cost. The Glycan Array is far from well optimized in our mind. There are three key limitations to an array-based approach with high throughput glycan-protein interaction profiling: 1) glycan arrays suffer from a systematic lack of sensitivity, 2) current array providers lack comprehensive glycan libraries with scalable synthetic routes, and 3) physiological glycan binding is intricate and complex and requires a lengthy analysis process. In Phase I we sought out to find solutions for each of these limitations. First, we developed a first of its kind Glycan Array that can analyze glycan binding avidity not just affinity. Second, we partnered with an elite group of chemists to produce a glycan library containing ~900 glycan structures, which is larger than that of the CFG and one of the largest ever. Third, we partnered with a group of data scientists and bioinformaticians to create a robust software package that automates the entire array data analysis process. Over the last three years we have formed valuable relationships with researchers and clinicians in research institutes, pharmaceutical companies and public health sectors. Z Biotech is perfectly positioned to facilitate the supply for increasing demand for biomedically relevant glycan arrays. In each of these endeavors Z Biotech sincerely thanks to the support of this SBIR grant. Now in Phase II we are seeking funds to continue to find solutions for these key limitations in Glycan Array. First, we will continue to invest heavily in R&D to ensure that our arrays offer superior insights compared to our competitors. We will also use funds from Phase II to scale up production to accommodate increasing demand. Second, we will team with Dr. Lei Li’s group at Georgia State University. Dr. Li is an outstanding glyco-scientist with experience in production of complex glycans, sugar nucleotides and glycan-related enzymes. With his effort we will maintain our current glycan library and add ~2,000 new glycan structures in Phase II. Third, we will complete Z Biotech’s custom software package. In Phase II we will bring this first of its kind glycan array data analysis package or solution to market.

项目概要/摘要 聚糖阵列已被证明是聚糖研究中的一个有价值的工具，相比之下，聚糖阵列可以提供以下结果： 在分析周转时间和财务方面以最低成本获得聚糖结合特异性的最佳视角 在我们看来，聚糖阵列还远没有得到很好的优化。基于阵列的技术存在三个关键限制。 高通量聚糖-蛋白质相互作用分析方法：1）聚糖阵列缺乏系统性 灵敏度，2) 当前的芯片供应商缺乏具有可扩展合成路线的综合聚糖库，以及 3) 生理聚糖结合错综复杂，需要漫长的分析过程。 首先，我们开发了第一个此类的聚糖阵列。 可以分析聚糖结合亲合力而不仅仅是亲和力 其次，我们与一组精英化学家合作。 生成包含约 900 个聚糖结构的聚糖文库，该文库比 CFG 和其中一个更大 第三，我们与一组数据科学家和生物信息学家合作创建了一个强大的软件。 在过去的三年里，我们已经形成了自动化整个阵列数据分析过程的软件包。 与研究机构、制药公司的研究人员和赞助人建立了宝贵的关系 Z Biotech 完全有能力促进公共卫生部门日益增长的需求的供应。 生物医学相关的聚糖阵列。 在每一项努力中，Z Biotech 衷心感谢 SBIR 拨款的支持，现在我们正处于第二阶段。 寻求资金继续寻找解决方案来解决 Glycan Array 的这些关键限制 首先，我们将继续寻找解决方案。 我们还将大力投资研发，以确保我们的阵列能够提供比竞争对手更出色的洞察力。 利用二期的资金扩大生产规模，以满足不断增长的需求。第二，我们将与合作伙伴合作。 佐治亚州立大学李雷博士团队是一位杰出的糖类科学家，在糖类领域拥有丰富的经验。 在他的努力下，我们将维持复杂聚糖、糖核苷酸和聚糖相关酶的生产。 我们目前的聚糖库，并在第二阶段添加约 2,000 个新的聚糖结构，我们将完成 Z Biotech 的项目。 在第二阶段，我们将带来第一个聚糖阵列数据分析包或定制软件包。 解决方案推向市场。