UNCOVERING HIDDEN IN VIVO RESONANCES USING EDITING BASED

使用基于编辑的方法揭示隐藏的体内共振

• 批准号: 7601632
• 负责人: MALGORZATA MARJANSKA
• 金额: $ 2.36万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601632
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Coupling; Funding; Glucose; Grant; Institution; Lasers; Lipids; Methods; Physiologic pulse; Protons; Pulse taking; Research; Research Personnel; Resources; Signal Transduction; Source; System; Techniques; United States National Institutes of Health; Water; base; in vivo; methyl lactate; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A novel single-shot spectral editing technique for in vivo proton NMR is proposed to recover resonances of low-concentration metabolites obscured by very strong resonances. With this new method, editing is performed by transferring transverse magnetization to J-coupled spins from selected coupling partners using a homonuclear Hartmann-Hahn polarization transfer with adiabatic pulses. The current implementation uses 1D-TOCSY with single-voxel localization based on LASER to recover the H1 proton of beta-glucose at 4.63 ppm from under water and the lactate methyl resonances from beneath a strong lipid signal. The method can be extended to further spin systems where conventional editing methods are difficult to perform.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 提出了一种用于体内质子NMR的新型单发频谱编辑技术，以恢复由于非常强的共振而掩盖的低浓度代谢产物的共振。使用这种新方法，通过将横向磁化剂转移到j耦合伴侣的旋转中，使用同核Hartmann-Hahn极化转移与绝热脉冲一起进行编辑。当前的实现使用基于激光的单素定位的1D焦点，以从水下4.63 ppm和从强脂质信号以下的乳酸甲基共振时回收β-葡萄糖的H1质子。该方法可以扩展到难以执行常规编辑方法的进一步自旋系统。