RNA-ENZYME RECOGNITION CODES IN AMINOACYL-TRNA SYNTHESIS AND TRNA MODIFICATION

氨基酰基-TRNA 合成和 TRNA 修饰中的 RNA 酶识别码

• 批准号: 7598082
• 负责人: MANAL A SWAIRJO
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598082
• 关键词: Alanine-Specific tRNA; Alanine-tRNA Ligase; Amino Acids; Amino Acyl-tRNA Synthetases; Anticodon; Base Pairing; Binding; Cells; Code; Codon Nucleotides; Complex; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Funding; Genetic Code; Grant; Institution; Ligase; Modification; RNA; Research; Research Personnel; Resources; Source; System; Transfer RNA; United States National Institutes of Health; base; novel; stem

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The genetic code is established by a system of 20 enzymes, aminoacyl-tRNA synthetases, which attach specific amino acids to tRNAs carrying anti-codon sequences cognate for the amino acid. Similarly, it has been recently proposed that the more numerous tRNA modification enzymes bind to their RNA substrates according to a complex recognition code. We want to understand the structural basis of highly specific tRNA recognition by these enzymes. A well studied synthetase is alanyl-tRNA synthetase which has been recently crystallographically deciphered (Swairjo et al., Mol. Cell., 2004, 13(6):829-841). This synthetase recognizes its cognate tRNA at a conserved G:U base pair in the acceptor stem, and is indifferent toward the tRNA anticodon. We have large crystals of AlaRS complexed with an RNA substrate mimicking the acceptor stem of tRNAAla. Similarly, YHDG and Ykvm are two novel enzymes involved in two different and specific tRNA modifications. We have crystals of Ykvm, and are currently persuing its tRNA complex with crystaliization trials.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 遗传密码是由 20 种酶（氨酰基-tRNA 合成酶）组成的系统建立的，这些酶将特定氨基酸附着到携带与该氨基酸同源的反密码子序列的 tRNA 上。同样，最近有人提出，更多的 tRNA 修饰酶根据复杂的识别代码与其 RNA 底物结合。我们想要了解这些酶高度特异性 tRNA 识别的结构基础。一种经过充分研究的合成酶是丙氨酰-tRNA合成酶，其最近已被晶体学破译(Swairjo等人，Mol.Cell.，2004，13(6)：829-841)。该合成酶在受体茎中保守的 G:U 碱基对处识别其同源 tRNA，并且对 tRNA 反密码子不感兴趣。我们有 AlaRS 的大晶体与模拟 tRNAAla 受体茎的 RNA 底物复合。同样，YHDG 和 Ykvm 是两种新型酶，参与两种不同且特定的 tRNA 修饰。我们有 Ykvm 晶体，目前正在通过结晶试验研究其 tRNA 复合物。