RNA-ENZYME RECOGNITION CODES IN AMINOACYL-TRNA SYNTHESIS AND TRNA MODIFICATION

氨基酰基-TRNA 合成和 TRNA 修饰中的 RNA 酶识别码

• 批准号: 7954229
• 负责人: PAUL R SCHIMMEL
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954229
• 关键词: Alanine-Specific tRNA; Alanine-tRNA Ligase; Amino Acids; Amino Acyl-tRNA Synthetases; Anticodon; Base Pairing; Binding; Cells; Code; Codon Nucleotides; Complex; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Funding; Genetic Code; Grant; Institution; Ligase; Modification; RNA; Research; Research Personnel; Resources; Source; System; Transfer RNA; United States National Institutes of Health; base; novel; stem; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The genetic code is established by a system of 20 enzymes, aminoacyl-tRNA synthetases, which attach specific amino acids to tRNAs carrying anti-codon sequences cognate for the amino acid. Similarly, it has been recently proposed that the more numerous tRNA modification enzymes bind to their RNA substrates according to a complex recognition code. We want to understand the structural basis of highly specific tRNA recognition by these enzymes. A well studied synthetase is alanyl-tRNA synthetase which has been recently crystallographically deciphered (Swairjo et al., Mol. Cell., 2004, 13(6):829-841). This synthetase recognizes its cognate tRNA at a conserved G:U base pair in the acceptor stem, and is indifferent toward the tRNA anticodon. We have large crystals of AlaRS complexed with an RNA substrate mimicking the acceptor stem of tRNAAla. Similarly, YHDG and Ykvm are two novel enzymes involved in two different and specific tRNA modifications. We have crystals of Ykvm, and are currently persuing its tRNA complex with crystaliization trials.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 遗传密码是由20个酶的系统，即氨基酰基-TRNA合成酶建立的，该系统将特定的氨基酸连接到携带抗氧化序列的TRNA，同源于氨基酸。同样，最近有人提出，根据复杂的识别代码，更多的tRNA修饰酶与其RNA底物结合。我们想了解这些酶高度特定的tRNA识别的结构基础。一个经过良好研究的合成酶是丙烷-TRNA合成酶，最近已被晶体学解密（Swairjo等，Mol。Cell。，2004，13（6）：829-841）。该合成酶在受体茎中识别其在保守的g：u碱基对处的同源tRNA，并且对tRNA反密码子无关紧要。我们有大的Alars晶体与模仿TRNAALA受体词干的RNA底物复合。同样，YHDG和YKVM是两种新型酶，涉及两种不同和特定的tRNA修饰。我们有YKVM的晶体，目前正在通过晶体化试验来说明其tRNA复合物。