MISSISSIPPI COBRE: P4: ID OF REWARDING CONSTITUENTS IN BOTANICAL EXTRACTS

密西西比 COBRE：P4：植物提取物中奖励成分的 ID

• 批准号: 7610760
• 负责人: KENNETH J SUFKA
• 金额: $ 14.08万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610760
• 关键词: Absence of pain sensation; Analgesics; Anxiety; Biological Assay; Biological Factors; Botanicals; Complex; Computer Retrieval of Information on Scientific Projects Database; Condition; Disease regression; Eligibility Determination; Funding; Goals; Grant; Institution; Mental Depression; Mississippi; Plant Extracts; Plants; Property; Protocols documentation; Research; Research Personnel; Resources; Rewards; Scientist; Score; Screening procedure; Series; Source; Therapeutic; Therapeutic Agents; United States National Institutes of Health; design; in vivo; in vivo Bioassay; preference

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overarching goal of this project is to develop a standardized protocol using a traditional in vivo bioassay to facilitate the screening of chemically complex botanical products with the goal of efficiently identifying plant constituents that possess rewarding or aversive properties that may ultimately limit their use as therapeutic agents. This project will utilize the expertise of natural product scientists at the National Center for Natural Products Research in providing the needed plant extracts and fractions for screening. The first aim is to use the conditioned place preference/avoidance (CPP/CPA) paradigm as an in vivo bioassay to screen botanical extracts, fractions and constituents and/or semisynthetic single entity compounds for putative reinforcing or aversive properties. The second aim is to incorporate and validate the use of multiple regression/correlation (MRC) design and analysis to pinpoint "activity" in a plant extract or fraction without the need to screen every single isolated constituent. In this statistical design, the CPP/CPA score serves as the predictor variable and varying constituent levels in a series of plant extracts as the criterion variable. The resulting analyses will predict which constituent or constituents are responsible for the rewarding/aversive properties within a plant. It is hoped that such an MRC design and analysis screening protocol generalizes to other in vivo screening assays (e.g., anxiety, depression, analgesia) in their search for putative therapeutic constituents contained in botanical products.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的总体目标是使用传统的体内生物测定法开发标准化方案，以促进化学复杂植物产品的筛选，目的是有效识别具有有益或厌恶特性的植物成分，这些特性可能最终限制其作为治疗剂的使用。该项目将利用国家天然产物研究中心天然产物科学家的专业知识，提供筛选所需的植物提取物和级分。 第一个目标是使用条件性位置偏好/回避（CPP/CPA）范式作为体内生物测定来筛选植物提取物、级分和成分和/或半合成单一实体化合物的推定增强或厌恶特性。 第二个目标是整合并验证多重回归/相关 (MRC) 设计和分析的使用，以查明植物提取物或级分中的“活性”，而无需筛选每个单独的成分。 在此统计设计中，CPP/CPA 分数作为预测变量，一系列植物提取物中不同的成分水平作为标准变量。 由此产生的分析将预测哪些成分或哪些成分对植物内的奖励/厌恶特性负责。 希望这种 MRC 设计和分析筛选方案能够推广到其他体内筛选测定（例如焦虑、抑郁、镇痛），以寻找植物产品中所含的假定治疗成分。