Neuropharmacology Core

神经药理学核心

基本信息

批准号：
10182736
负责人：
KENNETH J SUFKA
金额：
$ 29.41万
依托单位：
UNIVERSITY OF MISSISSIPPI
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-15 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10182736
关键词：
Acute Animal Model Antiepileptic Agents Anxiety Award Behavioral Binding Biochemical Biological Biological Assay Body Temperature Businesses Cannabinoids Catalepsy Cell Culture Techniques Cells Centers for Disease Control and Prevention (U.S.)Centers of Research Excellence Characteristics Cognition Collaborations Communities Core Facility Development Differentiation and Growth Education Ensure Enzymes Equipment Experimental Designs Fee-for-Service Plans Feedback G-Protein-Coupled Receptors Goals Grant In Vitro Infrastructure Lead Leadership Locomotion Mental Depression Mississippi Modeling Molecular Monitor Monoamine Oxidase A Natural Products Neuraxis Neurons Neuropharmacology Neurosciences Nociception Opioid Pharmacology Pharmacy Schools Phase Phenotype Positioning Attribute Postdoctoral Fellow Property Publications Quality Control Rattus Research Research Personnel Resource Sharing Rodent Scientist Services Signal Pathway Structure Structure of thyroid parafollicular cell Surveys Techniques Toxic effect Training Universities Zebrafish base behavior test behavioral pharmacology biological systems drug development drug seeking behavior esterase experience experimental study flexibility in vitro Assay in vivo instrumentation meetings mouse model multidisciplinary neuropeptide FF neuroprotection neurotoxicity outreach programs radioligand receptor binding response synthetic drug

项目摘要

PROJECT SUMMARY/ABSTRACT The Center of Research Excellence in Natural Products Neuroscience (CORE-NPN) at The University of Mississippi School of Pharmacy (UM), initiated ten years ago through a COBRE grant, has developed a multidisciplinary team committed to evaluating the effects of natural products on the central nervous system (CNS). The Neuropharmacology Research Core of the COBRE CORE-NPN program represents a shared resource serving biomedical investigators / users with specialized services and training in neuropharmacological bioassays based on (a) competitive radioligand receptor binding, (b) modulation of molecular/biochemical target functions (c) cell-based phenotypic & functional responses and (d) in vivo behavioral pharmacology. This was originally two Cores, in vitro and in vivo pharmacology, in Phases I and II, with strong utilization by investigators and strong collaboration between the two. In Phase III, it has been determined that these Cores would best serve the program as one functional unit. Furthermore, by the end of Phase III, the Core is projected to be largely sustainable with efforts focused on understanding neuropharmacological properties of lead biological extracts or purified compounds and how they interact with biological systems. Efforts have been focused on expanding the core capabilities by increasing both in vitro and in vivo assays that are available to users, thereby increasing the numbers of projected users served. A fee-for-service/chargeback business model will be implemented during Phase III to ensure sustainability post Phase III. Specific Core services include radioligand binding and functional assays for cannabinoid, opioid, and Neuropeptide FF G protein-coupled receptors (GPCRs), as well as enzyme assays, phenotypic neuronal cell cultures-based assays, and analysis of neuropharmacological signaling pathways. Moreover, the Core offers a battery of acute behavioral rodent and zebrafish assays to evaluate natural product extracts and purified compounds in vivo. New in vitro assays and animal models will continue to be developed to support user needs, which ultimately broadens the overall scope and user-base for the Core. The Core is positioned to become a leader in neuropharmacological services based on natural product and synthetic drug development campaigns. We plan to achieve this through the following Specific Aims: 1) To enhance the research of the COBRE CORE-NPN and the associated community through state-of-the-art equipment, cutting-edge techniques, and scientific expertise; 2) To advance an infrastructure of developmental services to facilitate COBRE CORE-NPN research and the associated community by continual refinement and addition of assays through user input. 3) To accelerate a long-term plan for sustainability of the Core through expansion of the user base, integration of a fee-for-service structure, institutional support, and adaptation to changes by monitoring the needs of the users.

项目概要/摘要英国大学天然产物神经科学卓越研究中心 (CORE-NPN) 密西西比药学院 (UM) 十年前通过 COBRE 资助发起，开发了一种多学科团队致力于评估天然产物对中枢神经系统的影响（中枢神经系统）。 COBRE CORE-NPN 计划的神经药理学研究核心代表了一个共享的为生物医学研究人员/用户提供神经药理学专业服务和培训的资源基于 (a) 竞争性放射性配体受体结合、(b) 分子/生化靶标调节的生物测定功能（c）基于细胞的表型和功能反应以及（d）体内行为药理学。这是最初有两个核心，体外和体内药理学，处于 I 期和 II 期，被研究人员广泛利用以及两者之间的强有力的合作。在第三阶段，已确定这些核心最适合程序作为一个功能单元。此外，到第三阶段结束时，预计核心将主要可持续发展，重点是了解先导生物提取物的神经药理学特性或纯化的化合物以及它们如何与生物系统相互作用。努力的重点是扩大通过增加用户可用的体外和体内测定的核心能力，从而提高预计服务的用户数量。期间将实施按服务收费/退款业务模式第三阶段确保第三阶段后的可持续性。具体的核心服务包括放射性配体结合和功能大麻素、阿片类药物和神经肽 FF G 蛋白偶联受体 (GPCR) 以及酶的检测测定、基于表型神经元细胞培养的测定以及神经药理学信号传导分析途径。此外，Core 还提供一系列急性行为啮齿动物和斑马鱼检测来评估体内天然产物提取物和纯化化合物。新的体外测定和动物模型将继续旨在支持用户需求，最终扩大核心的整体范围和用户基础。 The Core 的定位是成为基于天然产品和药物的神经药理学服务的领导者。合成药物开发活动。我们计划通过以下具体目标来实现这一目标： 1) 通过最先进的技术加强 COBRE CORE-NPN 和相关社区的研究设备、尖端技术和科学专业知识； 2）推进发展基础设施建设通过不断完善和促进 COBRE CORE-NPN 研究和相关社区的服务通过用户输入添加测定。 3) 通过以下方式加快核心可持续发展的长期计划扩大用户基础、整合按服务付费的结构、机构支持以及适应通过监控用户的需求来进行更改。