Mechanisms of Corneal Nerve Repair

角膜神经修复机制

• 批准号: 9910407
• 负责人: Mark I Rosenblatt
• 金额: $ 40.08万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9910407
• 关键词: Affect; Anatomy; Angiogenic Factor; Animal Model; Binding; Biological Assay; Blindness; Blinking; Blood Vessels; Cataract Extraction; Cell Line; Cells; Chemical Injury; Cornea; Corneal Abrasion; Corneal Diseases; Corneal Injury; Corneal Neovascularization; Cultured Cells; Cytometry; Data; Debridement; Defect; Development; Diabetes Mellitus; Dry Eye Syndromes; Electrophysiology (science); Endothelial Cells; Endothelial Growth Factors Receptor; Epithelial; Epithelium; Esthesia; Eye diseases; Growth; Growth Factor; Hair; Health; Heterodimerization; Homo; Human; Human body; Image; Immunoprecipitation; In Vitro; Individual; Infection; Injury; KDR gene; Keratopathy; Knock-out; Laceration; Lamellar Keratoplasty; Laser In Situ Keratomileusis; Lead; Ligands; Mediating; Mediator of activation protein; Modeling; Molecular; Morphology; Mus; Nerve; Nerve Degeneration; Nerve Plexus; Nerve Regeneration; Neurons; Neurotrophic Keratitis; Notch Signaling Pathway; Operative Surgical Procedures; PIK3CG gene; Pathway interactions; Pattern; Penetrating Keratoplasty; Perforation; Peripheral; Peripheral Nerves; Phosphorylation; Physiological; Population; Receptor Activation; Role; Sensory; Signal Pathway; Site; Stress; Structure; Structure of trigeminal ganglion; Techniques; Tissues; Trauma; Trigeminal nerve structure; Tyrosine; VEGFA gene; Vascular Endothelial Growth Factor B; Vascular Endothelial Growth Factors; Western Blotting; angiogenesis; arm; corneal regeneration; corneal repair; corneal surgery; eye dryness; ganglion cell; in vivo; inhibitor/antagonist; knock-down; melting; nerve injury; nerve supply; neurogenesis; neuronal survival; neutralizing antibody; prevent; receptor; repaired; response; restoration; small hairpin RNA; synergism; wound

PROJECT SUMMARY The cornea is the most densely innervated tissue in humans. Peripheral corneal nerves regenerate follow injury. Our hypothesis is that VEGF is a critical determinant of corneal nerve regeneration after injury and that the signaling pathways which mediate neurogenesis are distinct from those which mediate angiogenesis. Aim 1 will characterize the mechanisms by which VEGF ligands mediate corneal nerve repair in vitro and in vivo. Aim 2 characterizes the ability of VEGF ligands to mediate repair in animal models of corneal injury.

项目概要 角膜是人类神经支配最密集的组织。角膜周边神经再生 受伤。我们的假设是 VEGF 是损伤后角膜神经再生的关键决定因素 介导神经发生的信号传导途径与介导血管发生的信号传导途径不同。目的 图1将描述VEGF配体在体外和体内介导角膜神经修复的机制。 目标 2 描述了 VEGF 配体介导角膜损伤动物模型修复的能力。

项目成果

Benzalkonium chloride, a common ophthalmic preservative, compromises rat corneal cold sensitive nerve activity.

• DOI: 10.1016/j.jtos.2022.07.012
• 发表时间: 2022-08-01
• 期刊: The ocular surface
• 作者: Evguenia Ivakhnitskaia;Vladislav Souboch;Valentina Dallacasagr;e;e;K. Mizerska;Elizaveta Souboch;Joy Sarkar;V. Guaiquil;Kuei;H. Hirata;M. Rosenblatt
• 通讯作者: M. Rosenblatt

Corneal lymphangiogenesis as a potential target in dry eye disease - a systematic review.

角膜淋巴管生成作为干眼病的潜在目标 - 系统评价。

• 发表时间: 2021
• 影响因子: 5.1
• 作者: Chennakesavalu, Mohansrinivas;Somala, Sri Raghurama R;Dommaraju, Sunil R;Peesapati, Meghna Priyanka;Guo, Kai;Rosenblatt, Mark I;Chang, Jin;Azar, Dimitri T
• 通讯作者: Azar, Dimitri T