Molecular Epidemiology of Oxidative Stress and Related Cancers in Black Women

黑人女性氧化应激及相关癌症的分子流行病学

基本信息

批准号：
9906186
负责人：
Chiranjeev Dash
金额：
$ 13.08万
依托单位：
GEORGETOWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-09 至 2022-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9906186
关键词：
3-nitrotyrosine 8-hydroxy-2&apos -deoxyguanosine Address African American Antioxidants Area Award Bioinformatics Biological Assay Biological Markers Biometry Breast Cancer Risk Factor Cancer Etiology Chronic Cohort Studies Colorectal Cancer Colorectal Neoplasms Complement Complex Cysteine DNA Data Data Analyses Development Diet Dietary Practices Dietary intake District of Columbia Epidemiology Equilibrium Etiology F2-Isoprostanes Failure Food Frequencies Glutathione Glutathione Disulfide Goals Individual Investigation K-Series Research Career Programs Lipids Macronutrients Nutrition Malignant Neoplasms Mammary Neoplasms Measurement Measures Mediating Mentorship Metabolic Methods Micronutrients Minority Minority Groups Modeling Molecular Epidemiology Molecular Epidemiology of Cancer National Cancer Institute Nitrogen Observational Study Oxidation-Reduction Oxidative Stress Oxygen Participant Pathogenesis Patient Self-Report Pattern Phase Population Population Study Positioning Attribute Prevention Process Prospective cohort study Proteins Proteomics Questionnaires Reactive Oxygen Species Reporting Research Research Personnel Research Training Risk Role Sampling Sum Systems Biology Technology Tissues Training Translational Research Variant Woman Women&apos s Health anticancer research base biomarker panel cancer chemoprevention cancer health disparity cancer initiation cancer prevention cancer risk carbonyl group carcinogenesis career cohort colon cancer risk cost design dietary antioxidant epidemiology study experience health disparity high risk human old age (65+)in vivo malignant breast neoplasm metabolomics metropolitan minority health nutritional epidemiology oxidation phase 2 study population based portability public health relevance recruit sample collection skills true biomarker tumor progression underserved minority

项目摘要

DESCRIPTION (provided by applicant): Oxidative stress is defined as an "imbalance in pro-oxidants and antioxidants with associated disruption of redox circuitry and macromolecular damage". Chronic oxidative stress is believed to increase the risk of many cancers such as breast and colorectal cancers. Despite being at increased risk of developing and dying from oxidative-stress related cancers, African-Americans are under-represented in population-based studies of oxidative stress and cancer. This is especially true for biomarker-based and nutritional epidemiology studies of oxidative stress. This career development award is designed to provide training and research experience to Dr. Dash to support his goal of becoming an independent researcher investigating the molecular epidemiology of cancers among minorities including cancer disparities. Proposed training plans include mentorship and coursework in proteomics, metabolomics, bioinformatics, and exploratory data analysis, filling gaps in research training related to studies among minorities and health disparity studies, and research considerations in recruitment and bio specimen collection from minorities in epidemiologic research. The research component of the proposal aims to address the research gaps mentioned above by (1) characterizing the systemic oxidative stress burden via measurement of a panel of known biomarkers of oxidative stress using proteomics and metabolomics technologies; (2) identifying dietary patterns associated with oxidative stress; and (3) investigating the association of these dietary patterns with risk of breast and colorectal cancers among African- American women. The study will be conducted in two phases. Phase 1 involves measurement of a panel of 6 oxidative stress biomarkers using proteomic and metabolomic approaches and describing the oxidative stress burden among African-American women without cancer. Dietary patterns related to the biomarkers of oxidative stress will also be identified usin dietary intake data self-reported by participants on food frequency questionnaires. Phase 2 of the study involves investigating the association of the oxidative stress-related dietary patterns developed in Phase 1 with breast and colon cancer risk in a large, nationally-representative, cohort study of Black women. Results from the study will support larger and more definitive studies on oxidative stress and cancer among African-American women. The proposed research complements the training and mentorship plans in proteomics, metabolomics, bioinformatics, biostatistics, and minority health research. With this award Dr. Chiranjeev Dash will combine his previous training in epidemiology with advanced training in metabolomics/proteomics, bioinformatics, and biostatistics, and gain experience in conduct of minority health research to establish an independent research career focusing on translational research on the molecular epidemiology of oxidative stress in cancer causation and prevention.

描述（由申请人提供）：氧化应激被定义为“促氧化剂和抗氧化剂的不平衡，伴有氧化还原回路的破坏和大分子损伤”，慢性氧化应激被认为会增加许多癌症的风险，例如乳腺癌和结直肠癌。尽管非洲裔美国人患氧化应激相关癌症和死亡的风险增加，但在基于人群的研究中代表性不足对于基于生物标记的氧化应激和营养流行病学研究尤其如此。该职业发展奖旨在为 Dash 博士提供培训和研究经验，以支持他成为研究分子流行病学的独立研究员的目标。拟议的培训计划包括蛋白质组学、代谢组学、生物信息学和探索性数据分析方面的指导和课程，填补与少数族裔研究相关的研究培训的空白。少数群体和健康差异研究，以及流行病学研究中少数群体招募和生物样本采集的研究考虑因素该提案的研究部分旨在通过以下方式解决上述研究空白：(1) 通过一组测量来表征系统氧化应激负担。使用蛋白质组学和代谢组学技术确定已知的氧化应激生物标志物；(2) 识别与氧化应激相关的饮食模式；(3) 研究这些饮食模式与乳腺癌和结直肠癌风险的关联；该研究将分两个阶段进行，第一阶段涉及使用蛋白质组学和代谢组学方法测量一组 6 种氧化应激生物标志物，并描述与非裔美国女性癌症相关的氧化应激负担。氧化应激的生物标志物也将通过参与者在食物频率问卷中自我报告的饮食摄入数据来确定，该研究的第二阶段涉及调查阶段中开发的氧化应激相关饮食模式的关联。一项针对黑人女性的大型全国代表性队列研究中的乳腺癌和结肠癌风险，该研究的结果将支持关于非裔美国女性氧化应激和癌症的更大规模、更明确的研究。凭借这一奖项，Chiranjeev Dash 博士将把他之前在流行病学方面的培训与在流行病学方面的高级培训结合起来。代谢组学/蛋白质组学、生物信息学和生物统计学，并获得进行少数族裔健康研究的经验，建立独立的研究生涯，专注于癌症因果关系和预防中氧化应激分子流行病学的转化研究。