GENETIC AND BIOLOGICAL VARIABLITITY IN MATERNAL-INFANT STRAINS OF HIV-1

HIV-1 母婴病毒株的遗传和生物变异性

• 批准号: 7605430
• 负责人: Maureen M Goodenow
• 金额: $ 0.12万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-23 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7605430
• 关键词: Accounting; Address; Adult; Biological; CD14 gene; CD4 Positive T Lymphocytes; Cells; Child; Computer Retrieval of Information on Scientific Projects Database; Frequencies; Funding; Genes; Genetic; Grant; HIV-1; Infant; Infection; Institution; Lung; Maps; Mothers; Neonatal; Neurologic; Numbers; Peripheral; Phenotype; Property; Research; Research Personnel; Resources; Source; Symptoms; Time; United States National Institutes of Health; Vaccines; Virus; base; design; in vivo; macrophage; monocyte; neonate; novel strategies; peripheral blood; prenatal; prevent; transmission process; virus characteristic

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Factors involved in maternal transmission of HIV-1 are poorly defined yet have critical importance in designing strategies to prevent prenatal infection. When and how maternal viruses are transmitted are difficult parameters to define because only 20 to 30% of infants born to HIV-1 infected mothers become infected. However, one approach involves defining the genotypic complexity and cytotropic properties of viruses in the infants. For example, viruses that infect infants may be macrophage tropic reflecting their ability to infect both CD4 T lymphocytes and monocyte-derived macrophages (MDM) in culture. Macrophage tropic viruses may account for the neurological symptoms or pulmonary complications that are more frequent among HIV-1 infants and children than among HIV-1 infected adults. Preliminary studies indicate that peripheral monocytes expressing CD14 are infected in neonates and infants, in contrast to adults. Specific aims of the proposed studies will address the frequency with which HIV-1 sequences are detected in neonatal CDD14 + monocytes, their persistence over time in infants and children, and the characteristics of the viruses isolated from CD14+ peripheral blood monocytes. The proposed studies involve a number of novel approaches to assess the virus in CD14+ monocytes and to map the determinants in HIV-1 genes that contribute to the infectivity of these cells. The studies provide the basis for relating genetic variability of HIV-1 that can contribute to the infectivity of these cells. The studies provide the basis for relating genetic variability of HIV-1 to phenotype of the virus in vivo and have significant implications for developing vaccine strategies to prevent prenatal transmission of HIV-1.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 HIV-1的孕产妇传播涉及的因素的定义很差，但在设计防止产前感染的策略中至关重要。 何时以及如何传播产妇病毒是很难定义的参数，因为只有20％至30％的HIV-1感染母亲被感染。 但是，一种方法涉及定义婴儿病毒的基因型复杂性和胞质特性。 例如，感染婴儿的病毒可能是巨噬细胞的热带，反映了它们在培养中感染CD4 T淋巴细胞和单核细胞衍生的巨噬细胞（MDM）的能力。 巨噬细胞热带病毒可能是HIV-1婴儿和儿童比HIV-1感染的成年人更常见的神经系统症状或肺部并发症。 初步研究表明，与成人相比，表达CD14的外周单核细胞在新生儿和婴儿中感染。 拟议的研究的具体目的将解决新生儿CDD14 +单核细胞中检测到的HIV-1序列的频率，它们在婴儿和儿童中的持久性以及从CD14 +外周血单核细胞中分离出的病毒的特征。 提出的研究涉及多种新方法来评估CD14+单核细胞中的病毒，并绘制有助于这些细胞感染性的HIV-1基因中的决定因素。 该研究为将HIV-1的遗传变异性提供了基础，这可能有助于这些细胞的感染性。 该研究为将HIV-1与体内病毒表型的遗传变异联系起来提供了基础，并对制定疫苗策略具有重要意义，以防止产前传播HIV-1。