EARLY MARKERS FOR BREAST CANCER RISK IN NIPPLE ASPIRATE FLUID

乳头抽吸液中乳腺癌风险的早期标志物

• 批准号: 7605396
• 负责人: LEE-JANE W LU
• 金额: $ 0.23万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7605396
• 关键词: Arts; Aspirate substance; Breast Cancer Detection; Carcinoembryonic Antigen; Cataloging; Catalogs; Computer Retrieval of Information on Scientific Projects Database; Diagnosis; Experimental Designs; Family history of; First Degree Relative; Funding; Gel; General Population; Grant; Hormones; Institution; Liquid substance; MALDI-TOF Mass Spectrometry; Malignant Neoplasms; Methods; Newly Diagnosed; Nipples; Numbers; Population; Procedures; Prostate-Specific Antigen; Proteins; Purpose; Research; Research Personnel; Resources; Risk; Risk Marker; Source; Techniques; Technology; Time; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; Woman; Women' s Group; cancer risk; clinical application; cost; malignant breast neoplasm; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brief Background: In recent years, nipple aspirate fluid (NAF) has been considered as a potential source for breast cancer risk markers. The main components of NAF are proteins, including various hormone-regulated proteins, and generally known cancer markers such as prostate specific antigen (PSA) and carcinoembryonic antigen (CEA). Currently, however, there is no established way to identify the specific NAF protein profiles associated with breast cancer risk. Specific Aims and Procedures: Protein profiles of NAF will be analyzed by state-of-the-art techniques, including two-dimensional gel electrophoresis (2-D gel) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The combined use of these two technologies has enabled us to study global protein profiles in low risk healthy women and these methods are time- and cost-efficient and could be easily adapted for clinical applications. Experimental Design: The NAF protein profiles will be studied and compared among three groups of women: 40 healthy women with no family history of breast cancer, 40 healthy women with first-degree relatives diagnosed with breast cancer, and 40 women with newly diagnosed breast cancer, including those with unilateral breast cancer, before treatment. Significance: The ultimate purpose of this study is to discover and catalog NAF proteins that are early markers for breast cancer risk. Identification of breast cancer risk marker in NAF may revolutionize breast cancer detection because it can be performed an unlimited number of times and non-invasively and thus provides us a tool to follow prospectively the general population and follow more frequently the higher risk populations.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 简短背景：近年来，乳头抽吸液（NAF）被认为是乳腺癌风险标记的潜在来源。 NAF的主要成分是蛋白质，包括各种激素调节的蛋白质，以及普遍已知的癌症标志物，例如前列腺特异性抗原（PSA）和癌链酯抗原（CEA）。但是，目前尚无识别与乳腺癌风险相关的特定NAF蛋白谱的确定方法。 具体目的和程序：NAF的蛋白质曲线将通过最新技术进行分析，包括二维凝胶电泳（2-D Gel）和基质辅助激光解吸/电离飞行时间质谱（ MALDI-TOF MS）。这两种技术的综合使用使我们能够研究低风险健康女性中的全球蛋白质谱，这些方法具有时间和成本效益，并且很容易适应临床应用。 实验设计：将研究NAF蛋白轮廓并在三组女性中进行比较：40名没有乳腺癌家族史的健康女性，40位被诊断为乳腺癌的一级亲属的健康女性，以及40名新诊断的乳腺癌女性，包括治疗前的单侧乳腺癌的患者。 意义：这项研究的最终目的是发现和分类NAF蛋白，这些蛋白是乳腺癌风险的早期标志物。 NAF中乳腺癌风险标志物的鉴定可能会彻底改变乳腺癌的检测，因为它可以无限次，并且非侵入性地进行，因此为我们提供了一种工具，可以预期跟随一般人群，并更频繁地跟随较高的风险人群。