ESSENTIAL AMINO ACIDS AND RESISTANCE EXERCISE IN AGING

必需氨基酸和抗衰老运动

基本信息

批准号：
7605426
负责人：
Blake B Rasmussen
金额：
$ 2.79万
依托单位：
UNIVERSITY OF TEXAS MED BR GALVESTON
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2008-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7605426
关键词：
Acquired Immunodeficiency Syndrome Age Aging Amino Acids Biopsy Blood Blood specimen Catheters Clinical Research Computer Retrieval of Information on Scientific Projects Database Condition Elderly Essential Amino Acids Exercise Experimental Designs Funding Grant Growth Hormones Hour Infection Institution Insulin Intervention Kidney Failure Leg Lifting Malignant Neoplasms Measures Muscle Muscle Proteins Operative Surgical Procedures Peripheral Physical Dependence Procedures Protein Biosynthesis Proteins Rate Recruitment Activity Research Research Personnel Resistance Resources Sampling Sepsis Signal Pathway Signal Transduction Source Stress Testosterone Thinking Trauma United States National Institutes of Health Upper arm Weight Weight Lifting base improved insight male men mortality older men response size stable isotope volunteer wasting

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Muscle wasting is a common phenomenon in many conditions, such as cancer, AIDS, trauma, sepsis, kidney failure, and is also particularly prevalent in the elderly. The loss of muscle mass leads to overall weakness, immobility and physical dependence, and can be responsible for an impaired response to stress, which is associated with higher mortality rates during infection, surgery, and trauma. Amino acids from protein and weight lifting can both increase muscle size. It has been shown that muscle growth post-exercise is less in the elderly as compared to young volunteers. However, there have been no studies that have examined the combined effect of amino acids and resistance exercise. Hypothesis: Providing amino acids following a bout of weight lifting exercise will improve short-term muscle growth in older men. However, we also think that this response will still be less than that seen in young healthy men. Specific Aims and Procedures (summary): To determine if 20 grams of essential amino acids ingested one hour following a bout of weight lifting exercise will stimulate short-term muscle growth in older men to the same extent as seen in the young. Experimental Design (summary): Six healthy young and older male volunteers will be recruited and screened at the General Clinical Research Center. We will begin each study by placing two peripheral catheters (one in each arm) and by infusing two stable isotopes to measure muscle protein synthesis. We will collect two muscle biopsies prior to exercise, one following a bout of weight lifting exercise on the legs (8 sets x 8 repetitions at 70% of the subjects maximal weight they can lift), and then again at 3 hours and 6 hours post exercise. One hour following the exercise session each subject will receive 20 grams of essential amino acids. We will then assess changes in blood amino acid content, hormone changes such as insulin and testosterone from the blood samples. Each muscle sample will be used to measure muscle protein synthesis and to assess changes in cell signaling pathways the control protein synthesis. Each study will begin around 7 am and will be completed by 4 pm. Significance (summary): These studies will provide insight into why muscle loss is occurring with age and will then help us to develop scientifically-based interventions for improving muscle size in conditions such as aging, trauma, cancer, and AIDS.

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。子项目及研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以在其他 CRISP 条目中表示。列出的机构是对于中心来说，它不一定是研究者的机构。背景：肌肉萎缩是许多疾病中的常见现象，例如癌症、艾滋病、创伤、败血症、肾衰竭，并且在老年人中尤其普遍。肌肉质量的损失会导致全身虚弱、行动不便和身体依赖，并可能导致对压力的反应受损，这与感染、手术和创伤期间较高的死亡率有关。蛋白质和举重中的氨基酸都可以增加肌肉大小。研究表明，与年轻志愿者相比，老年人运动后的肌肉生长较少。然而，还没有研究检验氨基酸和抗阻运动的综合作用。假设：举重运动后提供氨基酸将改善老年男性的短期肌肉生长。然而，我们也认为这种反应仍然低于年轻健康男性的反应。具体目标和程序（摘要）：确定在举重运动后一小时摄入 20 克必需氨基酸是否会刺激老年男性的短期肌肉生长，其程度与年轻人相同。实验设计（概要）：将在综合临床研究中心招募并筛选六名健康的年轻和老年男性志愿者。我们将通过放置两根外周导管（每只手臂各一根）并注入两种稳定同位素来测量肌肉蛋白质合成来开始每项研究。我们将在运动前收集两次肌肉活检，一次在腿部举重运动后进行（8 组 x 8 次重复，受试者最大重量的 70%），然后在运动后 3 小时和 6 小时再次收集锻炼。锻炼后一小时，每位受试者将获得 20 克必需氨基酸。然后，我们将评估血液样本中血液氨基酸含量的变化以及激素变化，例如胰岛素和睾酮。每个肌肉样本将用于测量肌肉蛋白质合成并评估控制蛋白质合成的细胞信号传导途径的变化。每项研究将于上午 7 点左右开始，下午 4 点完成。意义（摘要）：这些研究将深入了解为什么肌肉会随着年龄的增长而减少，然后帮助我们开发基于科学的干预措施，以在衰老、创伤、癌症和艾滋病等情况下改善肌肉大小。