Pilot Projects Component (Pilot Proj 2: Guccione)

试点项目组件（试点项目 2：Guccione）

• 批准号: 7657168
• 负责人: Ann Arvin
• 金额: $ 11.82万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7657168
• 关键词: Behavior; Biological Process; Cell physiology; Classification; Clinical Protocols; DNA Microarray Chip; DNA Microarray format; Data; Disease; Enrollment; Event; Gene Expression; Genes; Genome; Human; Imagery; Immune response; Immunization; Individual; Knowledge; Laboratories; Lesion; Life; Maps; Microarray Analysis; Modeling; Monkeys; Pathogenesis; Pattern; Pilot Projects; Smallpox; Smallpox Viruses; Surrogate Markers; Vaccinated; Vaccination; Vaccines; Vaccinia; Vaccinia Vaccine; Vaccinia virus; Viral; cDNA Arrays; cohort; design; emergency service responder; human data; insight; nonhuman primate; programs; research study; response

lot project 2a. Transcriptional microarray analysis of human responses to smallpox vaccination. In the component of this project to be done in the Relman laboratory, we will use cDNA microarrays to characterize global gene expression patterns in response to smallpox vaccination in a pilot cohort of vaccinees enrolled in approved clinical protocols to evaluate vaccinia vaccines or who are being vaccinated as 'first responders'. The value of systematic studies of global gene expression programs is now well established in providing new insights into normal cellular physiology and disease pathogenesis. DNA microarrays provide a rapid and efficient way to build a detailed map of the gene expression program underlying a biological process, even in the absence of well-developed prior knowledge or models of its behavior. These experiments will allow us to build a comprehensive picture, across thousands of genes, of the human response to smallpox vaccination. These patterns can later be used to assess adverse vaccination events, and develop new surrogate markers for a protective vaccine response that does not rely on visualization of a Jennerian lesion. Although beyond the scope of this proposal, these data will create a framework within which to compare protective and non-protective vaccine responses in a non-human primate model of smallpox, and derive patterns of protection from the human data using data from monkeys challenged with live smallpox virus. Our long-term objective is to use genome-wide expression patterns as a means of predicting protective vaccine responses against smallpox, as well as adverse.responses to vaccine, in humans early after immunization. Our Specific Aims follow from this background and rationale.

批次项目2a。人类对天花的反应的转录微阵列分析 疫苗接种。 在该项目的组成部分中，我们将使用cDNA 微阵列以响应天花疫苗接种而表征全局基因表达模式 疫苗的试点队列批准的临床方案以评估疫苗疫苗或谁是 被接种为“第一响应者”。全球基因表达系统研究的价值 现在，计划在提供正常细胞生理和疾病的新见解方面已建立了良好的建立 发病。 DNA微阵列提供了一种快速有效的方法来构建基因的详细地图 即使没有发达的先验，生物过程的表达程序也 其行为的知识或模型。这些实验将使我们能够建立全面的图片， 在数千个基因中，人类对天花疫苗接种的反应。这些模式以后可能是 用于评估不良疫苗接种事件，并为保护性疫苗开发新的替代标记 不依赖詹纳尼亚病变的可视化的反应。虽然超出了这个范围 提案，这些数据将创建一个框架，以比较保护性和非保护性 天花的非人类灵长类动物模型中的疫苗反应，并从 人类数据使用来自活的天花病毒挑战的猴子的数据。我们的长期目标是 使用全基因组表达模式作为预测保护性疫苗反应的一种手段 免疫后早期人类中的天花以及对疫苗的反应。我们的具体 目的是从这种背景和理由遵循的。