System for guided imaging and characterization of neural circuits

神经回路引导成像和表征系统

• 批准号: 7537519
• 负责人: JACOB R GLASER
• 金额: $ 27.2万
• 依托单位: MICROBRIGHTFIELD, LLC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-10 至 2009-09-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7537519
• 关键词: Abbreviations; Alexa fluor 546; Area; Arts; Brain; Cells; Characteristics; Class; Collection; Computer software; Data; Dendritic Spines; Development; Devices; Distal; Effectiveness; Electrodes; Environment; Equipment; Feedback; Foundations; Future; Image; Intellectual Property; Legal patent; Life; MFC; Marketing; Methods; Microscope; Microscopic; Monitor; Morphology; Movement; Neurons; Neurophysiology - biologic function; Neurosciences; Neurosciences Research; Optics; Phase; Positioning Attribute; Preparation; Public Health; Radial; Relative (related person); Research; Research Personnel; Scanning; Slice; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Specimen; Structure; Synapses; System; Technology; Time; Tissues; Tracer; Water; base; biocytin; computerized; dentate gyrus; design; fluorophore; innovation; interest; lens; lucifer yellow; micromanipulator; nervous system disorder; neural circuit; neurotoxicology; novel; prototype; reconstruction; research and development; research study; sensor; software development

DESCRIPTION (provided by applicant): We propose the Sirius workstation, an innovative microscope system combining the capabilities of automated neuron reconstruction using guided image collection, cell electrophysiological recording, and cell filling in live tissue. Sirius will be the first commercially available system to a) enable direct recording of single distal synaptic connections formed between different areas, b) facilitate repeated monitoring of potential alterations in neuronal morphology during or as a result of stimulation, and c) permit simultaneous recording of one or more neurons formed between different areas in brain slices identified based on neuronal structure. This system will allow significant advancements in integrated studies of neural function and structure in basic neuroscience as well as in neurotoxicology and pharmacological research and development, representing clear progress beyond the state-of-the-art. PUBLIC HEALTH RELEVANCE The proposed project will enable new important studies in neuroscience research which are not currently feasible. A system that combines the capabilities of guided automated neuron reconstruction, cell electrophysiological recording and cell filling in live tissue will allow researchers to more accurately identify cells within a neural circuit, even those located at a distance from the original cell. The overall effect of this project will permit the collection of highly relevant data in integrated studies of neural function and structure in basic neuroscience, neurotoxicology, pharmacological research, and related fields involving research in neurological disease.

描述（由申请人提供）：我们提出了Sirius Workstation，这是一种创新的显微镜系统，结合了使用指导图像收集，电池生理记录和活细胞中的细胞填充的自动神经元重建功能。 Sirius将是第一个商业上可用的系统，a）可以直接记录不同区域之间形成的单个远端突触连接，b）促进在刺激期间或刺激过程中反复监测神经元形态的潜在变化，以及c）允许同时记录基于神经元识别的脑浆液中不同区域的或多个神经元。该系统将允许在基本神经科学以及神经毒理学和药理研究与开发中的神经功能和结构的综合研究中取得重大进步，这代表了最先进的明显进步。公共卫生相关性该拟议项目将使目前不可行的神经科学研究中的新重要研究。一个结合了引导自动神经元重建功能，细胞电生理记录和活细胞中细胞填充的系统，将使研究人员能够更准确地识别神经回路中的细胞，即使是距离原始细胞距离的细胞。该项目的总体效果将允许在基本神经科学，神经毒理学，药理学研究和相关领域的神经功能和结构的综合研究中收集高度相关的数据，这些研究涉及神经系统疾病的研究。