PROTEIN & NUCLEIC ACID CORE FACILITY

蛋白质

• 批准号: 7561497
• 负责人: KRISHNA BAKSI
• 金额: $ 12.77万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561497
• 关键词: Address; Area; Behavioral; Biological; Cell physiology; Computer Retrieval of Information on Scientific Projects Database; Contract Services; Core Facility; DNA Damage; Data; Educational workshop; Faculty; Fee-for-Service Plans; Funding; Future; Grant; Hand; Institution; Kinetics; Maintenance; Molecular; Molecular Biology Techniques; Neurons; Neuropharmacology; Nucleic Acid Core Facility; Nucleic Acids; Proteins; Research; Research Personnel; Resources; Services; Source; System; Techniques; Toxin; Training; United States National Institutes of Health; computerized; cost; improved; medical schools; member; programs; protein expression; protein purification; receptor; repaired; research and development; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The research performed at the UCC medical school are in different fields from behavioral neuropharmacology, repair mechanism, kinetics of DNA damage, neuronal and muscular receptor studies, cellular physiology and the analysis of toxin with its receptor. The Protein and Nucleic Acid Core Facility (PNACF) of the CMBC likes to aid in the transition from the more classical studies to a more molecular and detailed analysis of the problems being addressed by its faculty members. PNACF will focus on faculty training, seminars, training on specialized molecular biology techniques, protein expression and protein purification and characterization. Therefore, the PNACF continues to be an important core facility for the maintenance and future development of research at this institution.The SPECIFIC AIMS for improving this facility are: 1. to continue to acquire the molecular tools needed to analyze basic biomedical problems in a more detailed and modern fashion. (2). to sponsor ?hand-on? workshops in molecular biological techniques and protein expression and protein purification techniques given by invited experts of the fields. This will help the UCC researchers to solve their problem and to obtain preliminary data in their areas of their research. (3) to have the facility for the protein expression and purification in the UCC, and to encourage the UCC faculty members to expand their research to the protein level. (4) to continue and expand the resource centre where UCC faculty can obtain technical informations and cost estimations for molecular biological techniques and services which are not available in the UCC. (5) to continue and improve the computerized analysis of nucleic acid and protein data, and (6) to implement a ? fee for service? plan that will be used for the service contract/repair for VersaDOC system of Bio-RAD. This is to start to get partial self-support for the PNACF component of the RCMI program.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在UCC医学院进行的研究是在行为神经药理学，修复机制，DNA损伤的动力学，神经元和肌肉受体研究，细胞生理学以及毒素和受体分析的不同领域。 CMBC的蛋白质和核酸核心设施（PNACF）喜欢有助于从更古典的研究过渡到对其教职员工解决的问题的更分子和更详细的分析。 PNACF将专注于教师培训，研讨会，专业分子生物学技术，蛋白质表达以及蛋白质纯化和表征。 因此，PNACF继续是该机构维护和未来研究发展的重要核心设施。改善此设施的具体目的是：1。继续以更详细和现代的方式获取分析基本生物医学问题所需的分子工具。 （2）。赞助商？分子生物学技术，蛋白质表达和蛋白质纯化技术的讲习班由受邀田间专家提供。这将有助于UCC研究人员解决他们的问题，并在其研究领域获得初步数据。 （3）拥有UCC中蛋白质表达和纯化的设施，并鼓励UCC教师将其研究扩展到蛋白质水平。 （4）继续并扩大了UCC教师可以在UCC中无法获得分子生物技术和服务的技术信息和成本估算的资源中心。 （5）继续并改善核酸和蛋白质数据的计算机化分析，以及（6）实施A？服务费？ 计划将用于Bre-Rad Versadoc系统的服务合同/维修。 这是为了开始为RCMI程序的PNACF组件提供部分自助力。