PROTEIN & NUCLEIC ACID CORE FACILITY

蛋白质

• 批准号: 8166200
• 负责人: KRISHNA BAKSI
• 金额: $ 12.01万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8166200
• 关键词: Address; Area; Behavioral; Biological; Blood Platelets; Cancer Biology; Caveolae; Caveolins; Cell physiology; Cells; Cellular biology; Chimeric Proteins; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Core Facility; DNA Vaccines; Data; Educational workshop; Endothelial Cells; Equipment; Faculty; Funding; Future; Genes; Grant; Hand; Immune system; Institution; Maintenance; Microgravity; Molecular; Molecular Biology Techniques; Muscle; Nanotechnology; Neurons; Neuropharmacology; Nucleic Acid Core Facility; Nucleic Acids; Parkinson Disease; Postdoctoral Fellow; Protein Analysis; Proteins; Publications; Research; Research Personnel; Resources; Services; Source; Steroids; Techniques; Toxin; Training; United States National Institutes of Health; Vaccine Production; computerized; cost; medical schools; protein expression; protein purification; receptor; research and development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The research performed at the UCC Medical School covers a wide range of areas including behavioral neuropharmacology, neuronal and muscular receptor studies to cellular physiology, neuronal and glial networking, endothelial cell biology, steroid actions in the immune system and the effect of microgravity on the immune system, caveolae and caveolin expression and the analysis of toxin with its receptor, Parkinson's Disease, DNA vaccine production, cancer biology and platelet function. The Protein and Nucleic Acid Core Facility (PNACF) will aid the UCC investigators in the transition from the more classical studies to a more molecular and up-to-date analysis of the problems being addressed by each investigators. PNACF will focus on faculty training, seminars, training on specialized molecular biology techniques, protein expression and protein purification and characterization. Therefore, the PNACF continues to be an important core facility for the maintenance and future development of research at this institution. Specific Aims are: (1) To continue helping researchers with training/service current molecular biology techniques and training other researchers including post-doctoral fellows and research assistants, on the use and maintenance of specialized equipments. (2) To introduce two powerful techniques, namely gene microarray and nanotechnology in collaboration with the core facilities of other institutions in the mainland USA. (3) To sponsor 'hand-on" workshops in molecular biological techniques, cell-free protein expression and fusion protein purification techniques given by the invited experts in each of these fields. This will help the UCC researchers to directly address their research problems and to obtain preliminary data for publications/presentations/external funding. (4) To continue to be a resource center where the UCC faculty can obtain technical information and cost estimation for molecular biological/protein analysis techniques and services that are not available at UCC. (5) To continue helping the researchers for the computerized analysis of nucleic acid and protein data.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 The research performed at the UCC Medical School covers a wide range of areas including behavioral neuropharmacology, neuronal and muscular receptor studies to cellular physiology, neuronal and glial networking, endothelial cell biology, steroid actions in the immune system and the effect of microgravity on the immune system, caveolae and caveolin expression and the analysis of toxin with its receptor, Parkinson's Disease, DNA vaccine生产，癌症生物学和血小板功能。 蛋白质和核酸核心设施（PNACF）将有助于UCC研究人员从更古典的研究过渡到对每个研究者所解决的问题的更分子和最新分析。 PNACF将专注于教师培训，研讨会，专业分子生物学技术，蛋白质表达以及蛋白质纯化和表征。 因此，PNACF仍然是该机构维护和未来发展的重要核心设施。具体目的是： （1）继续帮助研究人员进行培训/服务当前的分子生物学技术，并培训其他研究人员，包括博士后研究员和研究助理，以使用和维护专业设备。 （2）引入两种强大的技术，即基因微阵列和纳米技术，与美国大陆其他机构的核心设施合作。 （3）赞助分子生物学技术的手工 - 无细胞的蛋白质表达和融合蛋白纯化技术，由受邀专家在这些领域的每个领域提供。这将帮助UCC研究人员直接解决他们的研究问题，并获得出版物/外部/外部资金的初步数据。 （4）继续成为一个资源中心，在该中心，UCC教师可以在UCC上获得分子生物/蛋白质分析技术和服务的技术信息和成本估算。 （5）继续帮助研究人员进行核酸和蛋白质数据的计算机化分析。