CACHET: Biomarkers Core

CACHET：生物标志物核心

• 批准号: 9271016
• 负责人: Jack Anthony Gilbert
• 金额: $ 4.28万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9271016
• 关键词: Anaerobic Bacteria; Androgens; Animals; Biological Assay; Biological Markers; Carbazoles; Chicago; Collaborations; Core Facility; Coupled; DNA Adducts; DNA Markers; Detection; Development; Dinoprostone; Endocrine Disruptors; Environment; Environmental Impact; Estrogens; Exposure to; Flame Retardants; Focus Groups; Food; Gas Chromatography; Health; Heavy Metals; High Pressure Liquid Chromatography; Human; Human Microbiome; Illinois; Individual; Inductively Coupled Plasma Mass Spectrometry; Isoprostanes; Laboratories; Lipid Peroxidation; Lipid Peroxides; Liquid Chromatography; Liquid substance; Mass Fragmentography; Measurement; Measures; Metagenomics; Metals; Methods; Needs Assessment; Nose; Oral cavity; Organic Chemicals; Oxidative Stress; Performance; Pesticides; Physiological; Polychlorinated Biphenyls; Procedures; Prostaglandin D2; Prostaglandins; Proteomics; Research; Research Personnel; Resources; Role; Sampling; Serum; Services; Skin; Soil; Source; Specimen; Stimulus; Technology; Tissues; Toxic Environmental Substances; Toxin; Universities; Urine; Validation; Water; analytical method; base; bisphenol A; design; dibenzofuran; discount; environmental chemical; experience; inflammatory marker; liquid chromatography mass spectrometry; member; metabolomics; microbial; microbiome; next generation sequencing; organochlorine pesticide; oxidation; oxidized lipid; pollutant; pressure; response; tandem mass spectrometry; toxicant; transcriptomics; urinary

1. BIOMARKERS CORE: ABSTRACT The Biomarkers Core provides essential analytical measurements in support of all focus groups of the ChicAgo Center for Health and EnvironmenT (CACHET). The overarching objectives of this Core are to provide CACHET members access to the following: a) routine analytical support enabling determination of exposure to environmental toxins as well as biomarkers indicating the physiological consequences of this exposure; b) new analytical methods and access to resources not previously available to CACHET members; and c) expertise for the design and interpretation of analytical measurements. Core access will be available equally and at discounted rates to all CACHET members, regardless of their institutional affiliation. Types of analyses and services will include 1) quantitative analysis of toxicants (such as pesticides and endocrine disruptors like bisphenol A) in biomedical specimens, and related biomarkers of exposure (including markers of inflammation such as the prostaglandins PGE2 and PGD2 and markers of oxidative stress such as 8-oxo-dG and 8-isoprostane PGF2α) using ultrahigh-pressure liquid chromatography combined with high performance tandem mass spectrometry (UHPLC-MS/MS) in the laboratory of Core Leader Dr. Richard van Breemen at UIC; 2) trace quantitative analysis of semi-volatile organic toxins such as PAHs, organochlorine pesticides, polyhalogenated carbazoles, and halogenated flame retardants in human and environmental samples using gas chromatography-tandem mass spectrometry (GC-MS/MS) in the laboratory of Dr. An Li at UIC; 3) quantitative analysis of metals (including Al, Mn, Fe, Ni, Cu, Zn, As, Se, Mo, Cd, Sn, Sb, Hg, and Pb) in environmental and biomedical specimens by using inductively coupled plasma mass spectrometry (ICP-MS) in the laboratory of Dr. C. Jin Qin at UofC; and 4) characterizing effects of environmental toxicants on the human microbiome in the laboratory of microbial ecologist Dr. Jack Gilbert at UofC. Some of these core resources, such as GC-MS/MS analysis of semi-volatile environmental toxins and effects of environmental toxins on the human microbiome have not been accessible previously to researchers at either UIC or UofC. Due to institutional contributions and CACHET support, these resources will be readily available to CACHET members at substantial discounts. Each laboratory providing services within this core is directed by an expert, and all of these resources will be coordinated by Dr. van Breemen, who has over 30 years of experience in the management of core facilities including MS-based resources.

1. 生物标志物核心：摘要 生物标志物核心提供必要的分析测量，以支持芝加哥所有焦点小组 健康与环境中心 (CACHET) 该核心的总体目标是提供 CACHET。 成员可以获得以下内容： a) 常规分析支持，能够确定暴露于 环境毒素以及指示这种暴露的生理后果的生物标志物；b) 新的； CACHET 成员以前无法获得的分析方法和资源；以及 c) 专业知识； 分析测量的设计和解释将平等地提供。 向所有 CACHET 成员提供折扣率，无论其分析类型和分析类型如何。 服务将包括 1) 有毒物质（如农药和内分泌干扰物等）的定量分析 生物医学样本中的双酚 A）以及相关的暴露生物标志物（包括炎症标志物） 例如前列腺素 PGE2 和 PGD2 以及氧化应激标记物，例如 8-oxo-dG 和 8-异前列腺素 PGF2α) 使用超高压液相色谱结合高性能串联质谱 UIC 核心负责人 Richard van Breemen 博士实验室的光谱测定 (UHPLC-MS/MS) 2) 痕量； 半挥发性有机毒素的定量分析，如多环芳烃、有机氯农药、多卤化物 使用气体检测人体和环境样品中的咔唑和卤化阻燃剂 UIC 李安博士实验室的色谱-串联质谱 (GC-MS/MS) 3) 定量） 环境和环境中的金属（包括 Al、Mn、Fe、Ni、Cu、Zn、As、Se、Mo、Cd、Sn、Sb、Hg 和 Pb）分析 在 Dr. 的实验室中使用电感耦合等离子体质谱 (ICP-MS) 分析生物医学样本。 C. Jinqin，加州大学伯克利分校；4) 描述环境毒物对人体微生物组的影响 UofC 微生物生态学家 Jack Gilbert 博士实验室的一些核心资源，例如 GC-MS/MS。 分析半挥发性环境毒素以及环境毒素对人体微生物组的影响 由于机构贡献和原因，UIC 或 UofC 的研究人员以前无法访问。 CACHET 支持，这些资源将以大幅折扣提供给 CACHET 会员。 在此核心内提供服务的实验室由专家指导，所有这些资源将 由拥有 30 多年核心设施管理经验的 van Breemen 博士负责协调 包括基于 MS 的资源。