HIV AND SUBSTANCES OF ABUSE LABORATORY CORE

艾滋病毒和滥用物质实验室核心

• 批准号: 6973825
• 负责人: EDDY RIOS-OLIVARES
• 金额: $ 24.62万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-29 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6973825
• 关键词: AIDS; Puerto Rico; behavioral /social science research tag; biomedical facility; chemokine; clinical research; cytokine; cytotoxicity; flow cytometry; genotype; human immunodeficiency virus; lymphocyte proliferation; minority institution research support; nucleic acid quantitation /detection; phenotype; substance abuse related disorder; tissue resource /registry; virus genetics; virus replication

The goal of this component is to establish a core laboratory with the needed human and physical resources to support research studies in the areas of HIV/AIDS and substances of abuse. For the next five years, specialized facilities will be made available to researchers for scientific studies in fields of immunology, drugs of abuse, HIV/AIDS and related infectious diseases. An in vitro system will be developed to test for the effect of addiction-related drugs and their interaction on HIV replication and the immune response under controlled laboratory conditions. The core lab will provide researchers with assays for nucleic acids detection, virus genotyping, flow-cytometric phenotyping, lymphocyte proliferation, cytotoxic and non-cytotoxic activity, cytokine and chemokine determinations, and drugs of abuse quantification. Consultants and mentors will continue to be available to users for the effective development of their projects and foster collaboration with external researchers. Moreover, support is requested to improve specimen repository, laboratory information system, electronic communication with Data Core, and compliance with HIPAA and IRB regulations. Furthermore, an institutionalizing effort of RCMI-supported infrastructure is described.

该组成部分的目的是建立具有所需人类和身体的核心实验室 支持在艾滋病毒/艾滋病领域的研究和虐待物质的研究。在接下来的五年中，研究人员将提供专门的设施，用于免疫学，虐待药物，艾滋病毒/艾滋病和相关传染病领域的科学研究。将开发一种体外系统来测试与成瘾相关药物的影响及其对受控实验室条件下的HIV复制和免疫反应的相互作用。核心实验室将为研究人员提供核酸检测，病毒基因分型，流动性表型，淋巴细胞增殖，细胞毒性和非胞毒性活性，细胞因子和趋化因子的测定以及滥用定量药物的测定法。顾问和导师将继续向用户提供有效开发项目的开发并促进与外部研究人员的合作。此外，请求支持以改善标本存储库，实验室信息系统，与数据核心的电子通信以及遵守HIPAA和IRB法规。此外，描述了RCMI支持的基础设施的制度化工作。