Neurosciencs Microarray Center

神经科学微阵列中心

• 批准号: 6625830
• 负责人: J H PATE SKENE
• 金额: $ 75.67万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6625830
• 关键词: RNA; biomedical facility; biotechnology; cooperative study; gene expression; information dissemination; laboratory mouse; laboratory rat; microarray technology; neurogenetics

DESCRIPTION (provided by applicant): DNA microarrays have emerged in just a few years as one of the most powerful approaches for assessing gene expression on a genome-wide scale. Despite its enormous potential, adoption of this technology by the neuroscience community has been slowed by the limited availability of microarrays, by limited access to training in microarray technologies, and by the extreme complexity and heterogeneity of cell types in the nervous system. At the same time, the enormous volume of data generated by microarray experiments has raised new issues regarding the sharing of microarray data beyond traditional peer-reviewed publication. We propose to create a Neuroscience Microarray Center to address these issues, and to increase access of the neuroscience community to DNA microarrays appropriate for analysis of gene expression in the nervous system. To increase the supply of DNA microarrays for neuroscience, the proposed center builds on the expertise of existing microarray core facilities with extensive experience in large-scale production and analysis of DNA microarrays. In concert with this array production component, the center will offer a formal course in the design, conduct and interpretation of microarray experiments, as well as ongoing advice to investigators. Research and development efforts will address two special requirements for neuroscience applications of DNA microarrays. First, we will develop DNA arrays with an enhanced ability to resolve major gene families involved in nervous system development, normal brain functions, and neurological diseases. Second, we will investigate best practices for the preparation of samples from defined nuclei, or identified populations of neurons. Particular emphasis will be given to determining optimal conditions for tissue processing and RNA extraction from human brain samples, and to an evaluation of alternative approaches for the isolation of small numbers of functionally or anatomically defined neurons. Finally, the proposed center will bring together experts in intellectual property law, research ethics, and neuroscience to address legal, ethical, and scientific aspects of microarray data sharing.

描述（由申请人提供）：DNA微阵列仅在几个 多年是评估基因表达的最有力的方法之一 全基因组量表。尽管具有巨大的潜力，但采用了这项技术 神经科学社区的可用性有限。 微阵列，通过有限的微阵列技术培训和通过 神经系统中细胞类型的极端复杂性和异质性。 同时，微阵列生成的大量数据 实验提出了有关分享微阵列数据的新问题 除了传统的同行评审出版物之外。我们建议创建一个 神经科学微阵列中心解决这些问题，并增加访问权限 神经科学界的DNA微阵列，适合分析 神经系统中的基因表达。增加DNA的供应 神经科学的微阵列，拟议中心建立在专业知识的基础上 现有的微阵列核心设施，具有丰富的大规模经验 DNA微阵列的生产和分析。与此阵列一致 生产组件，该中心将提供设计正式课程， 进行和解释微阵列实验以及持续的建议 致调查员。研发工作将解决两个特别的问题 DNA微阵列的神经科学应用的要求。首先，我们会的 开发具有解决主要基因家族的能力增强的DNA阵列 参与神经系统发展，正常的大脑功能和 神经疾病。其次，我们将研究最佳实践 从定义的核中制备样品，或确定的种群 神经元。特别强调确定最佳条件 用于组织加工和从人脑样品中提取RNA，然后 评估隔离少量的替代方法 在功能或解剖上定义的神经元。最后，拟议的中心将 将知识产权法，研究伦理和 神经科学解决微阵列的法律，道德和科学方面 数据共享。