Neurosciences Microarray Center

神经科学微阵列中心

• 批准号: 7293636
• 负责人: J H PATE SKENE
• 金额: $ 115.17万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7293636
• 关键词: Address; Archives; Area; Behavior; Biological; Biological Assay; Brain; Brain region; Cell Nucleus; Cell Separation; Class; Clinical; Collaborations; Collection; Communities; Complement; Computer software; Core Facility; Custom; DNA Microarray Chip; DNA Microarray format; Data; Data Analyses; Data Set; Databases; Development; Diagnostic; Disease; Ensure; Event; Funding; Gene Expression; Genes; Genomics; Human; Individual; Injury; Lasers; Left; Manufacturer Name; Measures; Microarray Analysis; Microdissection; Mus; National Institute of Mental Health; Nervous system structure; Neurons; Neurosciences; Neurosciences Research; Numbers; Oligonucleotides; Online Systems; Pattern; Personal Satisfaction; Physiological; Physiology; Play; Population; Preparation; Printing; Procedures; RNA; Rattus; Research Infrastructure; Research Personnel; Resources; Role; Running; Sampling; Series; Services; Source; Staging; Standards of Weights and Measures; Technology; Time; Transcript; base; cell type; density; design; experience; human data; improved; interest; member; nervous system development; programs; research and development; research study; tissue processing; tool; user-friendly

DESCRIPTION (provided by applicant): DNA microarrays provide a powerful approach for measuring gene expression on a genomic scale, allowing researchers to visualize changes in gene expression as a function of development, experience, disease, injury or physiological events. To facilitate the application of microarray technologies to critical problems in neuroscience, NINDS and NIMH in 2002 launched a consortium of three centers offering DNA microarrays and related services to the neuroscience community. The consortium provides standard DNA microarrays and services to all NINDS- and NIMH-funded investigators, and has developed a centralized database and analysis website that captures and archives MIAME-compliant data for experiments run through the consortium. This database provides a resource for individual investigators as well as a growing public set of microarray results relevant to basic and translational neurosciences. We propose here to continue as an integral component of this consortium, offering microarray platforms complementary to those provided by the other centers, and expanding the scope of consortium services to provide support at all stages of a microarray experiment. At the stage of sample preparation, we have established a new laser microdissection facility to meet the specialized needs of neuroscientists using microarrays to analyze specific populations of neural cells, small brain regions, or subcellular domains. Coordination with the other centers will ensure that samples prepared in the laser microdissection facility are suitable for analysis on any of the microarray platforms offered by the consortium. To extend support beyond the initial collection of microarray data, we propose to expand the tools and guidance available through the consortium website to assist investigators in analyzing and interpreting their own or public microarray results. Particular emphasis will be on tools to help investigators move beyond a simple list of differentially expressed genes to interpretations of the functional or diagnostic implications of the results. In collaboration with others in the consortium, we will continue research and development programs to enhance the representation on consortium arrays of transcripts involved in nervous system development, physiology and diseases, and to populate the consortium database with expression data for human and mouse nervous systems assayed across a series of developmental time points and brain regions on multiple microarray platforms. The proposed services build on a strong infrastructure and operating procedures developed in the first two years of the consortium to increase access of neuroscientists to established and emerging microarray technologies, and to enhance the value of those technologies in neuroscience research.

描述（由申请人提供）： DNA微阵列提供了一种在基因组量表上测量基因表达的强大方法，使研究人员可以将基因表达的变化可视化，这是发展，经验，疾病，损伤或生理事件的函数。为了促进微阵列技术在神经科学的关键问题中的应用，Ninds和NIMH于2002年成立了一个由三个中心组成的财团，为神经科学社区提供了DNA微阵列和相关服务。该财团为所有由NINDS和NIMH资助的研究人员提供了标准的DNA微阵列和服务，并开发了一个集中式数据库和分析网站，该网站捕获和档案符合迈阿密的数据，以供通过财团运行的实验。该数据库为个别研究人员以及与基本和转化神经科学相关的公共微阵列结果提供了资源。我们在这里建议继续作为该财团的组成部分，提供与其他中心提供的互补的微阵列平台，并扩大财团服务的范围，以在微阵列实验的各个阶段提供支持。在样品制备的阶段，我们建立了一个新的激光显微解剖设施，使用微阵列来满足神经科学家的专业需求，以分析神经细胞，小脑区域或亚细胞域的特定种群。与其他中心的协调将确保在激光显微解剖设施中制备的样品适合在财团提供的任何微阵列平台上进行分析。为了扩展支持最初的微阵列数据的支持，我们建议扩大通过财团网站可用的工具和指南，以协助调查人员分析和解释其自己或公共的微阵列结果。特别重点将放在工具上，以帮助研究人员超越差异表达基因的简单列表，以解释结果的功能或诊断含义。在财团中与其他人合作，我们将继续研究和开发计划，以增强对神经系统开发，生理和疾病涉及的成绩单阵列的代表，并用人类和小鼠神经系统的表达数据填充财团数据库，这些数据库是人类和小鼠神经系统的表达数据。拟议的服务建立在财团的前两年中开发的强大基础设施和操作程序，以增加神经科学家对建立和新兴的微阵列技术的获取，并增强这些技术在神经科学研究中的价值。