Modernization/operational improvements to basic centrifugation infrastructure of the Shared Instruments Pool in the Department of Biochemistry University of Colorado Boulder

科罗拉多大学博尔德分校生物化学系共享仪器池基本离心基础设施的现代化/运营改进

• 批准号: 10533249
• 负责人: Annette H Erbse
• 金额: $ 14.25万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-15 至 2023-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10533249
• 关键词: Acceleration; Accountability; Age; Basic Science; Biochemistry; Biomedical Engineering; Biomedical Research; Biotechnology; Cells; Centrifugation; Charge; Chemical Engineering; Colorado; Computers; Core Facility; Custom; DNA; Data; Diagnosis; Equipment; Equus caballus; Faculty; Floor; Free Will; Funding; Future; G Forces; Grant; Graph; Harvest; Individual; Infrastructure; Institutes; Interruption; Location; Manuals; Methods; Minor; Modeling; Modernization; Monitor; Nature; Organelles; Paper; Preparation; Procedures; Productivity; Proteins; RNA; Reaction Time; Reproducibility; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Role; Running; Safety; Sampling; Speed; Students; System; Time; Torque; Touch sensation; United States National Institutes of Health; Universities; Work; aged; base; cost; empowered; flexibility; handheld mobile device; improved; instrument; instrumentation; negative affect; programs; repaired; risk minimization; success

Project Summary/Abstract The Shared Instrument Pool (SIP) core facility in the Department of Biochemistry at CU-Boulder provides a combination of cutting-edge instrumentation and well-maintained basic research instrumentation (e.g., high- speed centrifuges), the latter of which are essential for biomedical research programs on campus. SIP widely and positively impacts many biomedical research programs at CU-Boulder, but this requires acquiring and maintaining modern, state-of-the-art instrumentation. Doing so enables streamlined workflows, better user support, higher throughput and productivity, and improves sample consistency, reproducibility, and data rigor. SIP currently maintains 5 high-speed, high-volume floor centrifuges and multiple different rotors, which are used by 12 major and many minor user labs spread over multiple departments. Many of the research projects that utilize the high-speed centrifuged are funded by grants from NIH, and nearly all are biomedical in nature. The current high-speed centrifuges are functional, but are all over 25 years old, which makes them difficult to maintain and they lack the up-to-data capabilities found on modern centrifuges. This is beginning to cripple efficient, safe, easy-to-support high throughput workflows in SIP. Therefore, funds are requested to modernize our centrifugation infrastructure with three Avanti JXN26 high-speed, large-volume centrifuges to replace three of our outdated models, and three rotors (two J-LITE® JLA-12.500 fixed-angle rotors and one JA30.50Ti fixed angle rotor). The proposal has a single Specific Aim, “Streamline and improve high-speed centrifugation in the Shared Instruments Pool”, and three Sub-Aims. 1.1 Improve user support, reproducibility, and centrifugation safety. The current centrifuges lack onboard computers, remote connectivity, the ability to save user profiles and customized methods, real-time run graphing, and simultaneous display of set and actual run conditions, all of which would be provided by the new high-speed centrifuges. These functionalities will minimize the potential for mistakes, run interruptions, sample loss, wasted time, and centrifuge damage and downtime. Moreover, the new centrifuges will be networked for method transfer and the MobileFuge App will allow users and SIP staff to check on runs in real time from anywhere and to receive messages concerning the runs. Finally, the new centrifuges and new rotors will detect any mismatch between the rotor and the chosen program and can stop a run to avoid damage. 1.2 Improve throughput. The new centrifuges will minimize the time needed to set up runs, and enable shorter runtimes by accessing higher g-forces and better k values, which together will decrease the time needed to achieve sample pelleting and allow higher throughput. 1.3 Minimize the administrative effort connected to centrifugation. Currently centrifuge usage is tracked manually via paper logs. The new centrifuges will store detailed usage data automatically, which will make usage tracking effortless and usage-based charging more accurate. This will free staff time for more important user and instrument support.

项目摘要/摘要 Cu-Boulder生物化学系的共享仪器池（SIP）核心设施提供了 尖端仪器和维护良好的基础研究仪器的结合（例如 速度离心），后者对于校园的生物医学研究计划至关重要。 sip广泛 并对Cu-Boulder的许多生物医学研究计划产生积极影响，但这需要认证和 维持现代，最先进的仪器。这样做可以启用精简的工作流程，更好的用户 支持，更高的吞吐量和生产力，并提高样本一致性，可重复性和数据严格。 SIP目前维持5个高速，高量的地板离心机和多个不同的转子 由12个主要和许多次要用户实验室分布在多个部门。许多研究项目 利用高速离心的基础是由NIH的赠款资助，几乎所有都是生物医学的。 当前的高速离心机功能正常，但已有25年的历史，这使它们难以维护 而且他们缺乏在现代离心机上发现的最新功能。这开始至关重要，安全，安全 SIP中易于支撑高吞吐量工作流程。因此，要求资金使我们的现代化 带有三个Avanti JXN26高速，大容量离心机的离心基础设施，以替换三个 我们过时的型号和三个转子（两个J-Lite®JLA-122.500固定角度转子和一个JA30.50TI固定 角转子）。该提案具有一个特定的目的：“简化和改善高速离心 共享仪器池”和三个子iams。1.1改善用户支持，可重复性和 离心安全。当前的离心机缺乏板载计算机，远程连接，保存的能力 用户配置文件和自定义方法，实时运行绘图以及设置和实际运行的简单显示 新的高速离心机将提供所有条件。这些功能将最小化 错误，跑步中断，样本损失，浪费时间以及离心损害和停机时间的潜力。 此外，新的离心机将被联网以进行方法传输，而移动设备将允许用户 然后sip员工从任何地方实时检查运行，并接收有关运行的消息。最后， 新的离心机和新转子将检测到转子和所选程序之间的任何不匹配，并且可以 停止跑步以避免损坏。 1.2改进吞吐量。新的离心机将使设置所需的时间最小 上运行，并通过访问更高的g forces和更好的k值来启用较短的运行时间 减少实现样品颗粒所需的时间并允许更高的吞吐量。 1.3最小化 与离心相关的行政工作。目前通过纸质日志手动跟踪离心机使用量。 新的离心机将自动存储详细的用法数据，这将使用法跟踪毫不费力，并且 基于用法的充电更准确。这将使员工时间免费获得更重要的用户和仪器支持。